Comparative analysis of qPCR and metagenomics for detecting antimicrobial resistance in wastewater: a case study

BMC Research Notes, Journal Year: 2025, Volume and Issue: 18(1)

Published: Jan. 7, 2025

The World Health Organization (WHO) has declared antimicrobial resistance (AMR) as one of the top threats to global public health. While AMR surveillance human clinical isolates is well-established in many countries, increasing threat intensified efforts detect antibiotic genes (ARGs) accurately and sensitively environmental samples, wastewater, animals, food. Using five ARGs 16S rRNA gene, we compared quantitative PCR (qPCR) metagenomic sequencing (MGS), two commonly used methods uncover wastewater resistome. We both by evaluating ARG detection through a municipal treatment chain. Our results demonstrate that qPCR was more sensitive than MGS, particularly diluted samples with low concentrations such oxidation pond water. However, MGS potentially specific less risk off-target binding concentrated raw sewage. analysis revealed multiple subtypes each gene which could not be distinguished qPCR; these varied across different sample types. findings affect conclusions can drawn when comparing types, terms inferring removal rates or origins genes. conclude appear suitable profile resistome other depending on research question type sample.

Language: Английский

---

Gut microbial communities and transcriptional profiles of black soldier fly (Hermitia illucens) larvae fed on fermented sericulture waste

Waste Management, Journal Year: 2025, Volume and Issue: 194, P. 158 - 168

Published: Jan. 12, 2025

Language: Английский

---

Characteristics of Intestinal Microbial Communities and Occurrence of Antibiotic Resistance Genes During Degradation of Antibiotic Mycelial Residues by Black Soldier Fly (Hermetia illucens L.) Larvae

Environmental Pollution, Journal Year: 2025, Volume and Issue: 371, P. 125940 - 125940

Published: Feb. 27, 2025

Language: Английский

---

Black soldier fly larvae mediate Zinc and Chromium transformation through the ZnuCBA and citric acid cycle system

Water Research, Journal Year: 2025, Volume and Issue: 280, P. 123483 - 123483

Published: March 11, 2025

Language: Английский

---

Adding Fruit Fermentation Liquid Improves the Efficiency of the Black Soldier Fly in Converting Chicken Manure and Reshapes the Structure of Its Intestinal Microbial Community

Insects, Journal Year: 2025, Volume and Issue: 16(5), P. 472 - 472

Published: April 29, 2025

This study evaluated how fruit fermentation liquid (FFL) enhances the conversion of chicken manure by black soldier fly larvae (BSFL) and modulates their gut microbiota. Three groups were tested: control (A: 300 g + 50 water), low-dose FFL (B: 25 high-dose (C: FFL). The results show that dry matter rate significantly increased 9.5% (p < 0.05), while feed-to-larvae ratio was reduced 1.02 0.01) in group C. NH3 emissions C decreased 24.48 mg·kg-1·DM (dry substrate) day-1 (24.48 mg per kilogram substrate day) 0.01), with suppressed H2S release. Gut microbiota analysis revealed abundance Proteobacteria (6.07% decrease C) enriching Actinobacteriota (4.68% increase) beneficial genera (Corynebacterium, Gallicola). Substrate microbial diversity improved, Firmicutes increasing 11.07% 4.83%, respectively, pathogenic Sphingobacteriaceae declining 21.16% day 7. likely introduced organic acids nutrients, enhancing larval digestion nutrient absorption inhibiting production harmful gases. These findings demonstrate optimizes BSFL-driven waste efficiency through modulation microbiota, offering a sustainable strategy for management contributing to circular agricultural systems.

Language: Английский

---

Gut microbiome of black soldier fly larvae for efficient use and purification of organic waste: An environmentally friendly development concept

The Innovation Life, Journal Year: 2025, Volume and Issue: unknown, P. 100134 - 100134

Published: Jan. 1, 2025

<p>The growing global population and increasing human demands have led to a rise in organic waste volumes. These wastes contain potential risk substances like pathogenic bacteria (PB), antibiotics (ABs), heavy metals (HMs), microplastics (MPs), which can threaten public health through direct contact or food chain transmission. Black soldier fly larvae (BSFL) emerged as promising solution for management crop-livestock integration. They efficiently reduce matter, produce high-quality proteins fats, generate green biofertilizers, mitigate harmful greenhouse gas emissions. However, the specific mechanisms by BSFL transform fertilizers, remain unclear, significantly limiting further development application of bioconversion technology. In recent years, numerous studies revealed that gut microbiota possess functions such promoting degradation substrate nutrients, regulating host nutrient synthesis metabolism, directly indirectly inhibiting proliferation PB, biodegrading cometabolizing ABs, mitigating mobility HMs, MPs. Therefore, exploring developing could be great significance optimizing efficiency transformation, effectively reducing waste, their wide applications biomedicine energy sectors. This review systematically summarizes composition, function, environmental impacts microbiota, focusing on roles transformation We believe in-depth exploration this field will advance integrated systems, is essential achieving humanity's sustainable goals.</p>

Language: Английский

---

Feeding black fly soldier larva using fermented solid residue generated from food waste three-phase separation

Journal of Material Cycles and Waste Management, Journal Year: 2025, Volume and Issue: unknown

Published: May 14, 2025

Language: Английский

---

Using black soldier fly larval frass to restore soil health

Bioresource Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132701 - 132701

Published: May 1, 2025

Language: Английский

---

Bioconversion of sulfamethazine-contaminated chicken manure by black soldier fly larvae: Effects on antibiotic resistance genes and microbial communities

Journal of Environmental Management, Journal Year: 2024, Volume and Issue: 371, P. 123206 - 123206

Published: Nov. 3, 2024

Language: Английский

---

Insect farming: a bioeconomy-based opportunity to revalorize plastic wastes

Environmental Science and Ecotechnology, Journal Year: 2024, Volume and Issue: 23, P. 100521 - 100521

Published: Dec. 24, 2024

Managing plastic waste is one of the greatest challenges humanity faces in coming years. Current strategies-landfilling, incineration, and recycling-remain insufficient or pose significant environmental concerns, failing to address growing volume residues discharged into environment. Recently, increasing attention has focused on potential certain insect larvae species chew, consume, partially biodegrade synthetic polymers such as polystyrene polyethylene, offering novel biotechnological opportunities for management. However, insect-assisted depolymerization incomplete, leaving amounts microplastics frass (or manure), limiting its use a soil amendment. In this perspective, we propose two-step bioconversion system overcome these limitations, using insects sustainably manage while revalorizing by-products (frass). The first step involves pyrolyzing microplastic-containing from mealworms (Tenebrio molitor larvae) fed plastic-rich diets produce biochar with enhanced adsorptive properties. second stage integrates entomocomposting organic residues, food waste, black soldier fly (Hermetia illucens) nutrient-rich substrates enriched carbon nitrogen. This integrated offers framework large-scale industrial applications, contributing bioeconomy by addressing both residue We critically examine advantages limitations proposed based current literature technology entomocomposting. Key research are identified, particularly concerning physiological toxicological processes involved, guide future efforts aimed at ensuring scalability sustainability innovative approach.

Language: Английский

---