Phylogenetic and functional diversity amongDrosophila-associated metagenome-assembled genomes DOI Creative Commons
Aaron A. Comeault, Alberto H. Orta,

David P. Fidler

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 22, 2024

Abstract Host-associated microbial communities can mediate interactions between their hosts and biotic abiotic environments. While much work has been done to document how microbiomes vary across species environments, less is known about the functional consequences of this variation. Here, we test for variation among drosophilid-associated bacteria by conducting Oxford Nanopore long-read sequencing generating metagenome-assembled genomes (MAGs) from six drosophilid fly collected in association with ‘anthropogenic’ environments North America, Europe, Africa. Using phylogenetic analyses, find that flies harbor a diverse microbiome includes core members closely related genera Gilliamella , Orbus Entomomonas Dysgonomonas others. Comparisons publicly available bacterial show many these are associated phylogenetically insect gut microbiomes. annotations predicted secondary metabolite biosynthetic gene clusters, MAGs belonging different orders content functions including metabolic capacity they respond environmental stressors. Our results provide evidence wild functionally communities. These findings highlight need quantify abundance function insect-associated others on performance

Language: Английский

On the tracks of an uninvited guest, the Asian tiger mosquito, Aedes albopictus in Cyprus DOI Creative Commons
Riccardo Piccinno,

Giulia Fiorenza,

Marlen I. Vasquez

et al.

Parasites & Vectors, Journal Year: 2025, Volume and Issue: 18(1)

Published: Feb. 4, 2025

Abstract Background Aedes albopictus , the Asian tiger mosquito, which is listed among world's 100 most dangerous invasive species, main vector of chikungunya, dengue and Zika viruses. This mosquito species has rapidly dispersed invaded much globe assisted by its life history traits high propagule pressure driven human activities. currently widespread across mainland Europe Mediterranean region, including islands. Cyprus remained free Ae. until October 2022, when specimens were recorded for first time in Limassol district, port area. Understanding processes associated with introduction, expansion establishment this primary importance to mitigate dispersal on island, implement control methods prevent disease outbreaks. A genetic analysis these collected district areas from Central was performed obtain a portrait demographic mosquitoes Cyprus. Methods We applied highly polymorphic simple sequence repeat (SSR) markers Italy, France, Switzerland, Balkans, Greece Turkey construct an SSR individual genotype dataset that would enable invasion pattern be traced. Bayesian clustering analyses using STRUCTURE BayesAss version 3 employed derive information degree ancestry Cypriot recent movements both within between areas. Results The appear no signs drift due founder effects. An ongoing detected, suggesting presence established, hidden adventive populations. These share those Balkans parts northern Italy border Adriatic Sea. Conclusions Considering trade connections port, Italian we hypothesise may involved incursion into As Balkan display competence CHIKV, questions arise about possible arbovirus outbreaks highlight need surveillance measures. Graphical

Language: Английский

Citations

1
The complexities of blood‐feeding patterns in mosquitoes and sandflies and the burden of disease: A minireview DOI Creative Commons
Fatma Bursalı, Mustapha Touray

Veterinary Medicine and Science, Journal Year: 2024, Volume and Issue: 10(5)

Published: Aug. 22, 2024

Mosquitoes and sandflies exhibit a wide range of blood feeding patterns, targeting vertebrate species, including birds, mammals, reptiles, amphibians, for proteins vital egg development. This broad host increases the opportunity them to acquire pathogens numerous debilitating-and-fatal diseases from various animal reservoirs, playing significant role in disease crossover between animals humans, also known as zoonotic transmission. review focuses on intricate blood-feeding habits these dipteran vectors, their sensory systems complex dance pathogen during We delve into influence sources spread by examining insect immune response its interplay with pathogens. The remarkable sense smell guiding towards food hosts is explored, highlighting multiple cues navigation. Finally, we examine challenges mosquito control strategies explore innovations this field, emphasizing need sustainable solutions combat global health threat. By understanding biology behaviour insects, can develop more effective protect ourselves mitigate burden vector-borne diseases.

Language: Английский

Citations

7
Exploring Mosquito Excreta as an Alternative Sample Type for Improving Arbovirus Surveillance in Australia DOI Creative Commons
Tess R. Malcolm, Melissa J. Klein, Karolina Petkovic

et al.

Pathogens, Journal Year: 2025, Volume and Issue: 14(1), P. 42 - 42

Published: Jan. 8, 2025

Current arbovirus surveillance strategies in Australia involve mosquito collection, species identification, and virus detection. These processes are labour-intensive, expensive, time-consuming can lead to delays reporting. Mosquito excreta has been proposed as an alternative sample type whole with potential streamline the pipeline. In this study, we investigated feasibility of Aedes aegypti a detection Dengue serotype 2 (DENV2). DENV2 could be detected from little one DENV2-infected spot, levels individual spots varying within between mosquitoes depending highly on viral load. Detectability was improved by pooling up 20 collecting into liquid substrate, followed concentration using magnetic nanoparticles. Virus improves quantification accuracy comparison unconcentrated samples increases amount material available for detection, expanding capabilities techniques higher limits type, coupled concentration, expands current toolbox improve Australia.

Language: Английский

Citations

0
Fungi on the cuticle surface increase the resistance of Aedes albopictus to deltamethrin DOI Open Access

Ju‐Ping Hu,

Si‐Jia Deng,

Lin Gu

et al.

Insect Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 19, 2025

Abstract Aedes albopictus ( Ae. ) is widely distributed and can transmit many infectious diseases, insecticide‐based interventions play an important role in vector control. However, increased insecticide resistance has become a severe public health problem, the clarification of its detailed mechanism matter urgence. This study found that target‐site metabolic could not fully explain field , there were likely other mechanisms involved. The 16S internal transcribed spacer sequencing revealed significant differences species compositions cuticle surface symbiotic bacteria fungi between deltamethrin (DM)‐resistant (DR) DM‐susceptible (DS) . Additionally, abundances Serratia spp. Candida significantly after DM treatment. Furthermore, 2 Rhodotorula mucilaginosa melibiosica 3 marcescens Klebsiella aerogenes sp.) isolated from DR use as their sole carbon source. After reinoculation onto DS R. C. enhanced Moreover, transcriptome surviving exposure gene expression cytochrome P450 enzymes glutathione‐S‐transferases increased, suggesting besides direct degradation, candidate degrading microbes also cause via indirect enhancement mosquito expression. In conclusion, we demonstrated involved development providing novel supplementary insights into mechanisms.

Language: Английский

Citations

0
Online Crowdsourced Data from iNaturalist Can Assist Monitoring of Invasive Mosquitoes DOI Creative Commons
Benjamin Cull

Insects, Journal Year: 2025, Volume and Issue: 16(2), P. 128 - 128

Published: Jan. 28, 2025

Invasive mosquitoes continue to spread, increasing the threat of mosquito-borne disease. Ongoing mosquito surveillance is necessary track introduction and establishment these species in new areas implement appropriate public health vector control measures. Contributions from citizen science initiatives have been an important component detecting, controlling, raising awareness invasive mosquitoes. The open-access biodiversity platform iNaturalist extensive source human observations wildlife, including arthropod vectors, can be a useful supplementary tool for passive surveillance. In this study, utility data support was assessed by examining distribution seasonal on four Aedes (IAS) Europe neighbouring countries. Almost 16,000 were examined across 62 countries; 13% identified as IAS, with further 2% considered probable IAS. These included 16 aegypti, 1582 albopictus, 373 japonicus, 58 koreicus. IAS present most known establishment, but potential regions spread also identified. results use low-cost assist existing

Language: Английский

Citations

0
Chijiahe virus, a novel mosquito-associated virus of the Iflaviridae family Found in Shandong Province, China DOI Creative Commons
Ke Lu,

Yongqin Wang,

Yanjun Wang

et al.

Heliyon, Journal Year: 2025, Volume and Issue: 11(4), P. e42532 - e42532

Published: Feb. 1, 2025

Highlights:•Chijiahe virus is a novel arbovirus and first identified from mosquitoes in Shandong province, 2022.•A total of 2910 were sampled Yantai area Province qRT-PCR performed to confirm the presence Chijiahe virus, with minimum infection rate 0.34%.•Phylogenetic analysis showed that most closely related Arthropod iflavirus, which belongs Iflaviridae.•Chijiahe was widely distributed local relatively conservative evolution.AbstractClimate change increasing threat arboviruses globally, amplifying their geographic distribution virulence. This poses grave risk public health as mosquito-transmitted viral infections resurge spread. Unbiased metagenomics plays crucial role identifying unknown pathogens monitoring significant ones. Using this approach, mosquito-associated named discovered. We collected 2,910 divided them into 65 pools based on species collection site. To ascertain existence we utilized nested PCR alongside real-time quantitative reverse transcription (RT-qPCR). Further virus's genomic characteristics phylogenetic relationships revealed its detection 10 out mosquito pools, yielding (MIR) 0.34%. The genome consists 9,654-nucleotide single-stranded RNA, containing an open reading frame (ORF) encodes essential proteins, including capsid protein, RNA helicase, RNA-dependent polymerase (RdRp). Pairwise distance high nucleotide identity among all obtained sequences. Phylogenetic demonstrated close relationship member family Iflaviridae. Additional analyses confirmed

Language: Английский

Citations

0
Lower Bird Evenness and Diversity Are Associated With Higher Usutu Prevalence in Culex pipiens Mosquitoes DOI Creative Commons

Víctor Manuel Rodríguez-Valencia,

Marie‐Marie Olive, Gilbert Le Goff

et al.

Zoonoses and Public Health, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 18, 2025

The mosquito-transmitted Usutu virus has spread in the last few years, becoming endemic several areas of Europe, such as southern French region Camargue. Our aim was to study relationships between presence viral agent Culex mosquitoes and structure bird communities context dilution effect. We carried out mosquito censuses selected localities across a land-use gradient screened pools for flaviviruses. focused on exploring how host diversity, richness, abundance evenness were associated with detection Cx. pipiens. detected seven pipiens, phylogenetic analysis identified lineage Africa 3, confirming its circulation. probability is lower diversity but higher richness pipiens abundances. Bird variable greatest explanatory power, being negatively related detecting supporting These results will help us better understand community risk mosquito-borne disease.

Language: Английский

Citations

0
Metagenome analysis of viruses associated with Anopheles mosquitoes from Ramu Upazila, Cox’s Bazar District, Bangladesh DOI Creative Commons
Tao Li, Mohammad Shafiul Alam, Yu Yang

et al.

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e19180 - e19180

Published: March 31, 2025

Bangladesh has a warm climate and landscapes favourable for the proliferation of mosquitoes. Mosquito-borne pathogens including malaria arthropod-borne viruses (arboviruses) remain serious threat to public health requiring constant vector control disease surveillance. From November 2018 April 2019, Anopheles mosquitoes were collected in three unions Ramu Upazila (sub-district) Cox’s Bazar District, Bangladesh. The mosquito specimens combined into pools based on date collection, household ID, sex. Metagenome next-generation sequencing was conducted elucidate diversity virus sequences each pool. Homology-based taxonomic classification phylogenetic analyses identified broad putative from 12 known families, with additional unclassified also likely present. Analysis male showed some these are capable being vertically transmitted. Moreover, many assembled share homology affinity segments sequenced genomes, may represent endogenous viral elements derived past evolutionary relationship between their hosts.

Language: Английский

Citations

0
Strengthening resilience to emerging vector-borne diseases in Europe: lessons learnt from countries facing endemic transmission DOI
Gina E. C. Charnley, Tilly Alcayna, Alex Almuedo-Riera

et al.

The Lancet Regional Health - Europe, Journal Year: 2025, Volume and Issue: 53, P. 101271 - 101271

Published: April 6, 2025

Language: Английский

Citations

0
Integrated Control of Aedes albopictus in a Residential Area Through a Community-Based Approach: NESCOTIGER, a Large-Scale Field Trial in Valencia, Spain DOI Creative Commons
Marcos López-de-Felipe, Pedro María Alarcón‐Elbal, Isaac García-Masiá

et al.

Pathogens, Journal Year: 2025, Volume and Issue: 14(4), P. 367 - 367

Published: April 8, 2025

Aedes albopictus has established populations in several European countries with a sustained spreading pattern through the continent. This invasive mosquito is public health threat due to its vector competence for multiple arboviruses. Notably, peri-domestic habits of this hematophagous insect greatly diminish efficacy regular control activities, as individuals may harbor private areas. The oviposition behavior can be exploited targeting adults and immature stages different types traps. An experimental integrated program, which included community-based mass trapping intervention areas, street-catch basins, an educational campaign, was developed infested residential area Valencia (Eastern Spain). Focusing on trapping, participating residents deployed traps belonging three modes action their gardens during season. A total 1028 families participated project, 2884 were deployed. study sector where adult lethal ovitraps used showed lowest collections, living reported highest satisfaction rates perception survey. deployment approach adulticidal trap type appears promising tool controlling Ae.

Language: Английский

Citations

0