Toxics, Journal Year: 2024, Volume and Issue: 12(12), P. 930 - 930

Published: Dec. 22, 2024

Bacteria of the genus Pseudomonas are most studied microorganisms that biodegrade persistent perfluoroorganic pollutants, and research their application for remediation environmental sites using biotechnological approaches remains relevant. The aim this study was to investigate ability a known destructor perfluorooctane sulfonic acid from accelerate enhance destruction long-chain perfluorocarboxylic acids (PFCAs), specifically perfluorooctanoic perfluorononanoic acid, in water soil association with strain P. mosselii 5(3), which has previously confirmed genetic potential degrading PFCAs. complete genome (5.86 million base pairs) 2,4-D, probably belonging new species Pseudomonas, sequenced, assembled, analyzed. genomes both strains contain genes involved defluorination fluorinated compounds, including haloacetate dehalogenase H-1 (dehH1) haloalkane (dhaA). 2,4-D also multicomponent enzyme system consisting dioxygenase component, an electron carrier, 2-halobenzoate 1,2-dioxygenase (CbdA) preference fluorides. able defluorinate PFCAs aqueous cultivation within 7 days, them as sole source carbon energy converting perfluorheptanoic acid. It assisted 5(3) convert perfluoropentanoic accelerating process by 24 h. In model experiment bioaugmentation artificially contaminated soil, degradation pseudomonads occurred faster deeper than individual strains, achieving degree biodestruction 75% over 60 main metabolite. These results great importance development methods biological recultivation organic pollutants protection understanding fundamental mechanisms bacterial interactions these compounds.

Language: Английский