Natural transformation of antibiotic resistance genes and the enhanced adaptability in bacterial biofilm under antibiotic and heavy metal stresses

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 490, P. 137740 - 137740

Published: Feb. 25, 2025

Language: Английский

---

Antibiotic Resistance Evolution of Bacterial Biofilm Via Natural Transformation Under Antibiotic and Heavy Metal Pressures

Published: Jan. 1, 2025

Bacterial biofilms can enhance tolerance to antibiotics, however, antibiotic-resistance evolution in under environmental stressors via horizontal gene transfer remains unclear. This study investigated the natural transformation mediated by extracellular DNA (eDNA) at single-cell level stresses of tetracycline, sulfamethoxazole, and Zn²⁺ explore biofilms. The sub-minimal inhibitory concentrations (MICs) enhanced antibiotic resistance genes (ARGs) 1.01-fold 4.62-fold B. subtilis 6.94-fold A. baylyi biofilms, respectively, compared unstressed conditions; meanwhile, biofilm growth increased 1.29-fold 1.48-fold, with transformation. These results demonstrated that ARGs bacterial adaptability stresses. stimulated polysaccharide production 1.06–3.15 times, offering protection against penetration. They also induced reactive oxygen species membrane permeability 1.02–2.83 1.02–2.04 promoting Additionally, exogenous SOS response-related introduced eDNA were upregulated 1.03–7.06 times recipient cells, facilitating functional regulation. findings reveal critical role sub-MIC stressors, providing a theoretical basis for controlling spreads.

Language: Английский

---

Size-specific mediation of the physiological responses and degradation ability of microalgae to sulfamerazine by microplastics

Aquatic Toxicology, Journal Year: 2025, Volume and Issue: 279, P. 107257 - 107257

Published: Jan. 22, 2025

Language: Английский

---

Observed Prevalence and Characterization of Fluoroquinolone-Resistant and Multidrug-Resistant Bacteria in Loggerhead Sea Turtles (Caretta caretta) from the Adriatic Sea

Antibiotics, Journal Year: 2025, Volume and Issue: 14(3), P. 252 - 252

Published: March 1, 2025

Background/Objectives: Antimicrobial resistance (AMR) is a major global health concern with profound implications for human, animal, and environmental health. Marine ecosystems are emerging as reservoirs of resistant bacteria due to contamination from anthropogenic activities. This study aimed investigate fluoroquinolone-resistant multidrug-resistant in loggerhead sea turtles (Caretta caretta). Methods: Cloacal swabs were collected 28 at rescue center southern Italy. Swabs cultured nutrient media supplemented enrofloxacin. Bacterial isolates underwent identification by MALDI-TOF, antimicrobial susceptibility testing, assessment multidrug resistance. Conjugation experiments evaluated the transferability enrofloxacin Results: Thirty-six enrofloxacin-resistant bacterial strains isolated 22 turtles. The identified species included Vagococcus fluvialis (13 strains), Citrobacter freundii (5), Escherichia coli (6), Pseudomonas mendocina (4). Thirty-five exhibited resistance, critically important antibiotics such imipenem observed C. Enterobacter faecium. showed no transfer genes. Conclusions: highlights prevalence caretta, implicating marine environments AMR. findings underscore need stricter regulation use monitoring dissemination ecosystems. These results contribute understanding AMR dynamics within One Health framework, emphasizing interconnectedness environmental, human

Language: Английский

---

Antibiotic Resistance Genes in Aquatic Systems: Sources, Transmission, and Risks

Aquatic Toxicology, Journal Year: 2025, Volume and Issue: 284, P. 107392 - 107392

Published: April 29, 2025

Language: Английский

---