Heat stress drives rapid viral and antiviral innate immunity activation in Hexacorallia DOI Creative Commons

Ton Sharoni,

Adrian Jaimes‐Becerra, Magda Lewandowska

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 22, 2024

Abstract The cnidarian class Hexacorallia, encompassing stony corals and sea anemones, plays a critical role in marine ecosystems. Coral bleaching, the disruption of symbiosis between zooxanthellate algae, is driven by climate change-induced seawater warming further exacerbated pathogenic microbes. However, how pathogens, especially viruses, contribute to accelerated bleaching remains poorly understood. present study utilizes model anemone Nematostella vectensis explore these dynamics creating transgenic line with reporter gene regulated sequences from two RIG-I-like receptor (RLR) genes involved antiviral responses. Under heat stress, showed significant upregulation, indicating that regulatory are indeed responsive thermal stress. Analyses transcriptome data N. , Exaiptasia diaphana (another anemone), coral Stylophora pistillata revealed stress-induced activation set bona fide immune-related conserved three species. Population-specific differences transcriptional responses were evident both depending on geographic origin. In presence or absence zooxanthellae also influenced immune expression. To test whether viruses themselves may this response under we subjected polyps variable periods stress measured transcript levels resident as well selected genes. While responded within 1-3 hours viral expression was already upregulated 30 minutes, suggesting their increase might be contributing elevated consequentially, demise organismal homeostasis. These findings highlight complex interplay environmental responses, symbiotic states Hexacorallia. Better understanding mechanisms could provide insights into pathways resilience changing climate.

Language: Английский

Heat stress drives rapid viral and antiviral innate immunity activation in Hexacorallia DOI Creative Commons

Ton Sharoni,

Adrian Jaimes‐Becerra, Magda Lewandowska

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 22, 2024

Abstract The cnidarian class Hexacorallia, encompassing stony corals and sea anemones, plays a critical role in marine ecosystems. Coral bleaching, the disruption of symbiosis between zooxanthellate algae, is driven by climate change-induced seawater warming further exacerbated pathogenic microbes. However, how pathogens, especially viruses, contribute to accelerated bleaching remains poorly understood. present study utilizes model anemone Nematostella vectensis explore these dynamics creating transgenic line with reporter gene regulated sequences from two RIG-I-like receptor (RLR) genes involved antiviral responses. Under heat stress, showed significant upregulation, indicating that regulatory are indeed responsive thermal stress. Analyses transcriptome data N. , Exaiptasia diaphana (another anemone), coral Stylophora pistillata revealed stress-induced activation set bona fide immune-related conserved three species. Population-specific differences transcriptional responses were evident both depending on geographic origin. In presence or absence zooxanthellae also influenced immune expression. To test whether viruses themselves may this response under we subjected polyps variable periods stress measured transcript levels resident as well selected genes. While responded within 1-3 hours viral expression was already upregulated 30 minutes, suggesting their increase might be contributing elevated consequentially, demise organismal homeostasis. These findings highlight complex interplay environmental responses, symbiotic states Hexacorallia. Better understanding mechanisms could provide insights into pathways resilience changing climate.

Language: Английский

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