EPO Enhances Adaptation to Hypoxic Environment in the Freshwater Teleost (Micropterus salmoides) through the PI3K/AKT Pathway DOI

Haoxiao Yan,

Liulan Zhao, Kuo He

et al.

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Hypoxia has become one of the most common environmental stress events in life history aquatic organisms due to accelerated global warming. Exploring adaptation mechanisms hypoxic environments is important deepen our understanding toxicology and design breeding programs. In this study, largemouth bass Micropterus salmoides exhibited greater adaptability after 4 weeks intermittent exposure (IHE), with O2 tension for loss equilibrium decreased from 1.17 ± 0.20 0.66 0.10 mg/L. Combined transcriptomics, biochemical detection, immunostaining results revealed that hypoxia-tolerant phenotype driven by IHE was strongly correlated activation erythropoietin (EPO). EPO promoted phosphoinositide-3-kinase (PI3K)/protein kinase B (AKT) signaling alleviate hepatic damage under acute (AHE) selectively regulating expression genes related antioxidant defense, antiapoptosis, cell proliferation, which plays an role adaptation. The inhibition impaired survival environments, but intervention PI3K agonist 740 Y-P reversed process. This novel finding provides insights into exploring how cope challenges hypoxia increasing risks.

Language: Английский

EPO Enhances Adaptation to Hypoxic Environment in the Freshwater Teleost (Micropterus salmoides) through the PI3K/AKT Pathway DOI

Haoxiao Yan,

Liulan Zhao, Kuo He

et al.

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Hypoxia has become one of the most common environmental stress events in life history aquatic organisms due to accelerated global warming. Exploring adaptation mechanisms hypoxic environments is important deepen our understanding toxicology and design breeding programs. In this study, largemouth bass Micropterus salmoides exhibited greater adaptability after 4 weeks intermittent exposure (IHE), with O2 tension for loss equilibrium decreased from 1.17 ± 0.20 0.66 0.10 mg/L. Combined transcriptomics, biochemical detection, immunostaining results revealed that hypoxia-tolerant phenotype driven by IHE was strongly correlated activation erythropoietin (EPO). EPO promoted phosphoinositide-3-kinase (PI3K)/protein kinase B (AKT) signaling alleviate hepatic damage under acute (AHE) selectively regulating expression genes related antioxidant defense, antiapoptosis, cell proliferation, which plays an role adaptation. The inhibition impaired survival environments, but intervention PI3K agonist 740 Y-P reversed process. This novel finding provides insights into exploring how cope challenges hypoxia increasing risks.

Language: Английский

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