Light-regulated microRNAs shape dynamic gene expression in the zebrafish circadian clock DOI Creative Commons
Zuo Wang, Shuang Wang,

Yi Bi

et al.

PLoS Genetics, Journal Year: 2025, Volume and Issue: 21(1), P. e1011545 - e1011545

Published: Jan. 8, 2025

A key property of the circadian clock is that it reset by light to remain synchronized with day-night cycle. An attractive model explore input in vertebrates zebrafish. Circadian clocks zebrafish peripheral tissues and even zebrafish-derived cell lines are entrainable direct exposure thus providing unique insight into function evolution regulatory pathways. Our previous work has revealed light-induced gene transcription a step entrainment as well enabling more general adaptation cells sunlight exposure. However, considerable evidence points post-transcriptional mechanisms, notably microRNAs (miRNAs), playing an essential role shaping dynamic changes mRNA levels. Therefore, does directly impact miRNAs? Are there light-regulated miRNAs if so, which classes do they target? To address these questions, we performed complete sequencing analysis transcriptome, encompassing small non-coding RNAs mRNAs. Importantly, identified sets miRNAs, many targets representing light-inducible mRNAs including genes involved redox homeostasis. We subsequently focused on light-responsive miR-204-3-3p miR-430a-3p predicted regulate expression cryptochrome ( cry1a cry1b ). Luciferase reporter assays validated target binding 3′UTRs , respectively. Furthermore, treatment mimics inhibitors two significantly affected their but also other core components clock1a bmal1b per1b per2 per3 ), rhythmic locomotor activity larvae. Thus, our identification reveals new intricacy multi-level regulation clockwork light.

Language: Английский

Light-regulated microRNAs shape dynamic gene expression in the zebrafish circadian clock DOI Creative Commons
Zuo Wang, Shuang Wang,

Yi Bi

et al.

PLoS Genetics, Journal Year: 2025, Volume and Issue: 21(1), P. e1011545 - e1011545

Published: Jan. 8, 2025

A key property of the circadian clock is that it reset by light to remain synchronized with day-night cycle. An attractive model explore input in vertebrates zebrafish. Circadian clocks zebrafish peripheral tissues and even zebrafish-derived cell lines are entrainable direct exposure thus providing unique insight into function evolution regulatory pathways. Our previous work has revealed light-induced gene transcription a step entrainment as well enabling more general adaptation cells sunlight exposure. However, considerable evidence points post-transcriptional mechanisms, notably microRNAs (miRNAs), playing an essential role shaping dynamic changes mRNA levels. Therefore, does directly impact miRNAs? Are there light-regulated miRNAs if so, which classes do they target? To address these questions, we performed complete sequencing analysis transcriptome, encompassing small non-coding RNAs mRNAs. Importantly, identified sets miRNAs, many targets representing light-inducible mRNAs including genes involved redox homeostasis. We subsequently focused on light-responsive miR-204-3-3p miR-430a-3p predicted regulate expression cryptochrome ( cry1a cry1b ). Luciferase reporter assays validated target binding 3′UTRs , respectively. Furthermore, treatment mimics inhibitors two significantly affected their but also other core components clock1a bmal1b per1b per2 per3 ), rhythmic locomotor activity larvae. Thus, our identification reveals new intricacy multi-level regulation clockwork light.

Language: Английский

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