High-Throughput qPCR Profiling of Antimicrobial Resistance Genes and Bacterial Loads in Wastewater and Receiving Environments DOI Creative Commons
Reshma Silvester,

Nick Woodhall,

William Nurmi

et al.

Environmental Pollution, Journal Year: 2025, Volume and Issue: unknown, P. 126096 - 126096

Published: March 1, 2025

Wastewater treatment plants (WWTPs) are hot spots for the acquisition and spread of antimicrobial resistance (AMR). This regional-based study quantified antibiotic genes (ARGs), mobile genetic elements (MGEs), bacteria in hospital community-derived wastewater receiving environments, using high-throughput qPCR (HT-qPCR). is first to apply Resistomap's Antibiotic Resistance Gene Index (ARGI) as a standardised metric find overall AMR level across different WWTPs. ARGI WWTPs ranged from 2.0 2.3, indicating higher relative ARG levels than mean European 2.0, but lower global 2.4. The highest diversity abundance ARGs were observed untreated community wastewater. reduction total during (0.2- 2 logs) (0.3-1.5 varied spatio-temporally Despite decrease bacterial treated effluents, substantial loads still released into environments. Notably, coastal sediments comparable those wastewater, most shared between highlighting impact discharge on these ecosystems. Sewage outfall exposure increased shellfish, emphasising risks shellfish hygiene. provides evidence inform policymaking, advanced methods combined sewer overflow (CSO) management mitigate release, protecting water users food chain.

Language: Английский

High-Throughput qPCR Profiling of Antimicrobial Resistance Genes and Bacterial Loads in Wastewater and Receiving Environments DOI Creative Commons
Reshma Silvester,

Nick Woodhall,

William Nurmi

et al.

Environmental Pollution, Journal Year: 2025, Volume and Issue: unknown, P. 126096 - 126096

Published: March 1, 2025

Wastewater treatment plants (WWTPs) are hot spots for the acquisition and spread of antimicrobial resistance (AMR). This regional-based study quantified antibiotic genes (ARGs), mobile genetic elements (MGEs), bacteria in hospital community-derived wastewater receiving environments, using high-throughput qPCR (HT-qPCR). is first to apply Resistomap's Antibiotic Resistance Gene Index (ARGI) as a standardised metric find overall AMR level across different WWTPs. ARGI WWTPs ranged from 2.0 2.3, indicating higher relative ARG levels than mean European 2.0, but lower global 2.4. The highest diversity abundance ARGs were observed untreated community wastewater. reduction total during (0.2- 2 logs) (0.3-1.5 varied spatio-temporally Despite decrease bacterial treated effluents, substantial loads still released into environments. Notably, coastal sediments comparable those wastewater, most shared between highlighting impact discharge on these ecosystems. Sewage outfall exposure increased shellfish, emphasising risks shellfish hygiene. provides evidence inform policymaking, advanced methods combined sewer overflow (CSO) management mitigate release, protecting water users food chain.

Language: Английский

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