Application of the Allium Test in Toxicity Studies of Lead and Copper: A Cytological Perspective DOI Creative Commons

Emilian Onisan,

Ioan Sărac,

C. Petolescu

et al.

Applied Sciences, Journal Year: 2025, Volume and Issue: 15(3), P. 1491 - 1491

Published: Feb. 1, 2025

The Allium test is a cytological method used to monitor the impact of heavy metals. It can be evaluate meristematic tissues and highlight abnormalities occurring during mitotic division, with advantage being both rapid economical. Copper lead are among most widespread metals in everyday life, mainly due worldwide expansion industrialization, present soil, water, air. Using sativum as bioindicator for this study, statistical analysis confirmed significant differences genotoxicity between two metals, reflected by inhibition activity (MI) or increased indices cellular (AI). Toxicity was dose- time-dependent copper exhibiting greater genotoxic effects than lead. caused reduction MI, even at relatively low concentrations, IC50 observed 0.50 mM after 72 h exposure. In contrast, lead, recorded from 0.75 advantages were demonstrated its simplicity high sensitivity detecting abnormalities. our experiment, chromosome such bridges, well isolated, delayed, sticky chromosomes, observed. addition, concentration 0.25 (72 exposure) exposure), abnormalities, including giant cells binucleated cells, identified.

Language: Английский

Application of the Allium Test in Toxicity Studies of Lead and Copper: A Cytological Perspective DOI Creative Commons

Emilian Onisan,

Ioan Sărac,

C. Petolescu

et al.

Applied Sciences, Journal Year: 2025, Volume and Issue: 15(3), P. 1491 - 1491

Published: Feb. 1, 2025

The Allium test is a cytological method used to monitor the impact of heavy metals. It can be evaluate meristematic tissues and highlight abnormalities occurring during mitotic division, with advantage being both rapid economical. Copper lead are among most widespread metals in everyday life, mainly due worldwide expansion industrialization, present soil, water, air. Using sativum as bioindicator for this study, statistical analysis confirmed significant differences genotoxicity between two metals, reflected by inhibition activity (MI) or increased indices cellular (AI). Toxicity was dose- time-dependent copper exhibiting greater genotoxic effects than lead. caused reduction MI, even at relatively low concentrations, IC50 observed 0.50 mM after 72 h exposure. In contrast, lead, recorded from 0.75 advantages were demonstrated its simplicity high sensitivity detecting abnormalities. our experiment, chromosome such bridges, well isolated, delayed, sticky chromosomes, observed. addition, concentration 0.25 (72 exposure) exposure), abnormalities, including giant cells binucleated cells, identified.

Language: Английский

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