Optimization of Microwave-Assisted Extraction of Polyphenols from Crataegus monogyna L.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(3), P. 357 - 357

Published: March 18, 2025

Hawthorns (Crataegus monogyna L.) contain numerous bioactive compounds, with its extracts demonstrating health benefits. This study focused on optimizing a more sustainable extraction method, specifically microwave-assisted (MAE), to obtain polyphenols from hawthorn leaves and flowers. HPLC/UV analysis identified key including gallic chlorogenic acids, isoquercetin, rutin, hyperoside, vitexin, quercetin. Principal component (PCA) assessed correlations between conditions, total phenolic content (TPC), compounds. PCA grouped conditions into three clusters, two remaining ungrouped. The highest isoquercetin contents were achieved at 60 °C for 10 min using 160-500 μm particles in 75% ethanol (20/1 ratio) or 50% 30/1 ratios). An ungrouped condition (60 °C, min, < 160 particles, ethanol, 20/1 produced higher TPC greater acid, hyperoside concentrations. antioxidant activity (AA) of the optimized 23 full factorial design, temperature, concentration, solvent-to-plant ratio as variables. Optimal MAE (S/Popt = 20.4 mL/g, Topt 65 EtOHopt 60%) yielded 116.23 ± 2.85 mg GAE/g DM an AA 237.6 6.33 TE/g Summarizing above, maximize phytocompound content, one-factor-at-a-time design parameters, but limitations led design. latter effectively AA, while revealed phenolics,

Language: Английский

Recent advancements in the bioactive alkaloids analysis in plant and biological specimen: From the perspective of activity, sample preparation, and analytical method selection

Journal of Chromatography B, Journal Year: 2025, Volume and Issue: unknown, P. 124592 - 124592

Published: April 1, 2025

Language: Английский