Advances in Clinical Medicine, Journal Year: 2025, Volume and Issue: 15(03), P. 608 - 613
Published: Jan. 1, 2025
Language: Английский
Cureus, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 13, 2025
Background Recently, polyetheretherketone (PEEK) has emerged as a promising implant material with some limitations. The objective of this investigation was to improve the hydrophilicity and biocompatibility PEEK through surface coating by nanoporous titanium oxide (TiO2) hyaluronic acid (HA), targeting higher degree cell viability, proliferation, differentiation, mineralization around implants. Methods Prior film deposition, substrates were treated argon plasma. Using plasma sputtering, 800 nm thick TiO2 coatings applied plasma-treated PEEK. then coated layer HA. discs seeded osteoblast cells. A total four groups, each containing eight discs, created. groups included uncoated PEEK, HA-coated HA control group, which contained cells only. 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) test, 4',6-diamidino-2-phenylindole (DAPI) staining method, alkaline phosphatase (ALP) activity, alizarin red used assess capacity. Results Osteoblast proliferation rates group (69.37±6.16), greater than (60.12±2.41), (53.25±2.37) (36.37±4.56) after 21 days according DAPI test. Additionally, ALP activity on (0.59±0.15, 2.18±0.23) disks (0.62±0.17, 1.43±0.16), (0.45±0.11, 0.84±0.24) (0.17±0.08, 0.32±0.16) seven days, respectively. Alizarin in (1.41±0.07, 1.72±0.11) significantly (1.24±0.11, 1.35±0.07), (1.20±0.10, 1.01±0.08) (1.00±0.09, 1.00±0.05) at both time intervals, However, increased viability (1.14±0.07, 1.14±0.07) more (1.10±0.40, 1.10±0.40), (0.99±0.07, 0.99±0.07) (1.00±0.01, 1.00±0.01) 72 hours Conclusion Double its biofunctional properties osteoblasts.
Language: Английский
Citations
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Advances in Clinical Medicine, Journal Year: 2025, Volume and Issue: 15(03), P. 608 - 613
Published: Jan. 1, 2025
Language: Английский
Citations
0