Cancer Immunotherapy with “Vascular-Immune” Crosstalk as Entry Point: Associated Mechanisms, Therapeutic Drugs and Nano-Delivery Systems DOI Creative Commons
Zhijie Jiang, Zhujun Fang, Dongsheng Hong

et al.

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 7383 - 7398

Published: July 1, 2024

Tumor vessels characterized by abnormal functions and structures hinder the infiltration immune antigen presentation of cells inducing formation an immunosuppressive microenvironment ("cold" environment). Vascular-targeted therapy has been proven to enhance stimulation effectiveness immunotherapy modulating "cold" microenvironment, such as hypoxia acidic microenvironment. Notably, a therapeutic strategy based on "vascular-immune" crosstalk can achieve dual regulation tumor system reprogramming (TME), thus forming positive feedback loop between From this perspective, we discuss factors angiogenesis TME formation. Building foundation, some vascular-targeted drugs will be elaborated upon in detail immunity. More importantly, focus cutting-edge nanotechnology view rational fabrication tailor-made nanosystems for efficiently enhancing immunotherapy.

Language: Английский

A novel long non-coding RNA XLOC_004787, is associated with migration and promotes cancer cell proliferation by downregulating mir-203a-3p in gastric cancer DOI Creative Commons

Renjie Miao,

Zhendong Yao,

Bingheng Hu

et al.

BMC Gastroenterology, Journal Year: 2023, Volume and Issue: 23(1)

Published: Aug. 12, 2023

Abstract Background Long noncoding RNAs (lncRNAs) have been identified as important regulatory factors implicated in a wide array of diseases, including various forms cancer. However, the roles most lncRNAs progression gastric cancer (GC) remain largely unexplored. This study investigates biological function and underlying mechanism novel lncRNA, XLOC_004787 GC. Methods The location GES-1 cells HGC-27 were detected by fluorescence situ hybridization (FISH) assay. expression levels assessed using quantitative real-time PCR (qRT-PCR) cell lines, GES-1, MGC-803, MKN-45, BGC-823, SGC-7901, cells. Functional assays such Transwell migration, counting kit-8 (CCK-8), colony formation experiments employed to analyze effects miR-203a-3p on migration proliferation. Protein associated with GC these lines examined Western blotting. intracellular localization β-catenin P-Smad2/3 was immunofluorescence (IF) Additionally, interaction between investigated dual luciferase Results localized at both cytoplasm nucleus Compared normal tissues cells, significantly upregulated highest lowest observed SGC-7901 respectively. Furthermore, reduced seen inhibit proliferation blotting analysis revealed that decrease correspondingly decreased N-cadherin, mmp2, mmp9, Snail, Vimentin, β-catenin, C-myc, Cyclin D1, TGF-β, while concurrently increasing E-cadherin expression. also diminished relation Smad2/3, P-Gsk3β comparison Gsk3β. nuclear entry lower Amplifying via pcDNA_XLOC_004787 suggested potential for promotion. Notably, found negatively regulate mir-203a-3p expression, binding sites two. Higher proliferation, enhance Conversely, suppression promotion Conclusions These results suggest XLOC_004787, be tissues, potentially promotes occurs through activation TGF-β Wnt/β-catenin signaling pathways EMT-related proteins. may influence modulating pathway adsorption inhibition mir-203a-3p.

Language: Английский

Citations

5
CD40 is expressed in the subsets of endothelial cells undergoing partial endothelial–mesenchymal transition in tumor microenvironment DOI Creative Commons
Kazuki Takahashi, Miho Kobayashi,

H. Katsumata

et al.

Cancer Science, Journal Year: 2023, Volume and Issue: 115(2), P. 490 - 506

Published: Dec. 18, 2023

Abstract Tumor progression and metastasis are regulated by endothelial cells undergoing endothelial–mesenchymal transition (EndoMT), a cellular differentiation process in which lose their properties differentiate into mesenchymal cells. The EndoMT through spectrum of intermediate phases, suggesting that some remain partial state exhibit an endothelial/mesenchymal phenotype. However, detailed analysis has been hampered the lack specific markers. Transforming growth factor‐β (TGF‐β) plays central role induction EndoMT. Here, we showed inhibition TGF‐β signaling suppressed human oral cancer cell xenograft mouse model. By using genetic labeling lineage, also established novel reporter system, (EMRECs), allow visualization sequential changes during TGF‐β‐induced Using EMRECs, characterized gene profiles multiple stages identified CD40 as EndoMT‐specific marker. expression was upregulated EndoMT, but decreased full Furthermore, single‐cell RNA sequencing tumors revealed enriched population expressing both Moreover, EMRECs enhanced expressed inhibits to present findings provide better understanding mechanisms underlying will facilitate development therapeutic strategies targeting EndoMT‐driven metastasis.

Language: Английский

Citations

4
Transforming growth factor-β signals promote progression of squamous cell carcinoma by inducing epithelial-mesenchymal transition and angiogenesis DOI Creative Commons

Haruka Ibi,

Kazuki Takahashi, Hiroyuki Harada

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2024, Volume and Issue: 714, P. 149965 - 149965

Published: April 17, 2024

At present, the molecular mechanisms driving progression and metastasis of oral squamous cell carcinoma (OSCC) remain largely uncharacterized. The activation transforming growth factor-β (TGF-β) signaling in tumor microenvironment has been observed various types cancer implicated their by enhancing migration invasion epithelial cells. However, its specific roles unexplored. In this study, we examined effects TGF-β on murine carcinoma, SCCVII cells vitro vivo. incubation with induced signals epithelial-mesenchymal transition (EMT). Notably, motility was increased upon signaling. RNA sequencing revealed upregulation genes related to EMT angiogenesis. Consistent these results, inhibition cell-derived primary tumors resulted suppressed Furthermore, identified six candidate factors (ANKRD1, CCBE1, FSTL3, uPA, TSP-1 integrin β3), whose expression cells, associated poor prognosis for patients head neck carcinoma. These results highlight role OSCC via multiple mechanisms, including angiogenesis, suggest novel therapeutic targets treatment OSCC.

Language: Английский

Citations

1
Special issue: TGF-β and epithelial-mesenchymal transition in cancer DOI
Peter ten Dijke, Kohei Miyazono,

Carl-Henrik Heldin

et al.

Seminars in Cancer Biology, Journal Year: 2024, Volume and Issue: 102-103, P. 1 - 3

Published: June 27, 2024

Language: Английский

Citations

1
Cancer Immunotherapy with “Vascular-Immune” Crosstalk as Entry Point: Associated Mechanisms, Therapeutic Drugs and Nano-Delivery Systems DOI Creative Commons
Zhijie Jiang, Zhujun Fang, Dongsheng Hong

et al.

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 7383 - 7398

Published: July 1, 2024

Tumor vessels characterized by abnormal functions and structures hinder the infiltration immune antigen presentation of cells inducing formation an immunosuppressive microenvironment ("cold" environment). Vascular-targeted therapy has been proven to enhance stimulation effectiveness immunotherapy modulating "cold" microenvironment, such as hypoxia acidic microenvironment. Notably, a therapeutic strategy based on "vascular-immune" crosstalk can achieve dual regulation tumor system reprogramming (TME), thus forming positive feedback loop between From this perspective, we discuss factors angiogenesis TME formation. Building foundation, some vascular-targeted drugs will be elaborated upon in detail immunity. More importantly, focus cutting-edge nanotechnology view rational fabrication tailor-made nanosystems for efficiently enhancing immunotherapy.

Language: Английский

Citations

1