International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 258, P. 129051 - 129051
Published: Dec. 28, 2023
Language: Английский
Vaccines, Journal Year: 2022, Volume and Issue: 10(8), P. 1200 - 1200
Published: July 28, 2022
The novel coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome 2 (SARS-CoV-2), has severely impacted human health and the management system globally. ongoing pandemic required development of more effective diagnostic strategies for restricting deadly disease. For appropriate management, accurate rapid screening isolation affected population is an efficient means containment decimation Therefore, considerable efforts are being directed toward robust techniques infections, including SARS-CoV-2. In this article, we have summarized origin, transmission, various utilized detection SARS-CoV-2 virus. These higher-end can also detect virus copy number in asymptomatic samples. Furthermore, emerging rapid, cost-effective, point-of-care devices capable large-scale COVID-19 discussed. Finally, some breakthrough developments based on spectroscopic diagnosis that could revolutionize field
Language: Английский
Citations
17
Biosensors and Bioelectronics X, Journal Year: 2023, Volume and Issue: 15, P. 100412 - 100412
Published: Oct. 20, 2023
We present a report on the electrochemical kinetics and sensing studies conducted flexible ITO-PET transducer, fabricated with new nanocomposite of L-cysteine coated iron oxide nanoparticles (LCys@Fe3O4 NPs) multi-wall carbon nanotubes (MWCNT), for first time. Synthesized via wet-chemical co-precipitation method, LCys@Fe3O4 NPs exhibit pure cubic phase. A these are prepared dispersion in chitosan (CS) solution along MWCNT, further drop-casted onto low-cost, sheet. The CS-MWCNT-LCys@Fe3O4/ITO-PET electrode shows higher surface roughness, compared to either MWCNT or modified counterpart. CS-MWCNT-LCys@Fe3O4 exhibits twofold enhancement peak anodic voltammetric current, tenfold decrease charge transfer resistance, as nanoparticles. Additionally, mathematical model is devised evaluate kinetic parameters (ks, α & DFe(II)) redox species K4[Fe(CN)6]/K3[Fe(CN)6] surface, confirming quasi-reversible electron process. couple this transducer an immunosandwich format facilitate rapid ultrasensitive measurement protein biomarkers. Proof-of-concept carried out by measuring SARS-CoV-2 spike glycoprotein biofunctionalized Ab1/CS-MWCNT-LCys@Fe3O4/ITO-PET immunoelectrodes within wide linear range (1pg mL-1-1μg mL-1). immunoelectrode encompasses limit detection 0.932 pg mL-1, sensitivity 0.499 μA pg−1 mL cm−2. Thus, presents excellent biosensing application, while can serve efficient, flexible, cost-effective platform which be utilized fabricate point-of-care biosensors.
Language: Английский
Citations
10
Heliyon, Journal Year: 2023, Volume and Issue: 9(2), P. e13190 - e13190
Published: Jan. 23, 2023
The performance of an automated commercial CRISPR/Cas based technology was evaluated and compared with routine RT-PCR testing to diagnose COVID-19. Suspected discharged COVID-19 cases were included tested CRISPR-based SARS-CoV-2 test assay using throat swab sputum specimens. diagnostic yield calculated the McNemar test. A total 437 patients for analysis, including (n = 171), 155), others 111). For diagnosis COVID-19, CRISPR-SARS-CoV-2 had a sensitivity specificity 98.2% (168/171) 100.0% (266/266), respectively; (171/171) respectively. No significant difference found in RT-PCR. In conclusion, comparable showed several advantages, such as short time, low cost, no requirement expensive equipment.
Language: Английский
Citations
9
Talanta, Journal Year: 2023, Volume and Issue: 268, P. 125337 - 125337
Published: Oct. 24, 2023
Language: Английский
Citations
9
International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 258, P. 129051 - 129051
Published: Dec. 28, 2023
Language: Английский
Citations
9