Au Nanoparticle/CoFc/Metal–Organic Framework as Enzyme-Free Dual-Signal Ratiometric Electrochemical Sensor for In-Situ Determination of Cell-Secreted H2O2 DOI

Jiangbo Dong,

Jilin Zheng,

Jingzhou Hou

et al.

ACS Applied Nano Materials, Journal Year: 2023, Volume and Issue: 6(13), P. 11630 - 11639

Published: June 15, 2023

In-situ determination of cell-secreted biomolecules is important for elucidating its regulation signal transduction pathways, but detecting these low-level endogenous difficult. Herein, using H2O2 as the detection object, we developed an ultrasensitive enzyme-free ratiometric electrochemical sensing platform in a simple and low-cost method in-situ H2O2, which ferrocene (Fc) acts internal reference probe nanoflower Au-Co-MOF probe. First, flower-like CoFc-MOF was synthesized situ on flexible carbon cloth (CC) by one-step hydrothermal method, then Au-CoFc-MOF/CC obtained electrodeposition gold nanoparticles. The measurement results show that presence increases reduction peak Co-MOF (−0.32 V), while Fc (+0.4 V) almost unchanged. By taking full advantage obviously separated potential Co-MOF, well-designed can reflect concentration based IH2O2 – Iblank/IFc value 0.5 μM–10 mM range, with limit low 32 nM, ∼15.6 times lower than from single method. In addition, also demonstrates excellent selectivity, reproducibility, stability, biocompatibility. More significantly, this substrate shows great application provides feasible strategy other active substances released living cells clinical diagnosis.

Language: Английский

Heterostructured BiVO4/CoPi nanoarrays as high-efficiency photoanode and AuPt nanodendrites as nanozyme for sensitive sensing of miRNA 141 DOI
Guiqing Wang,

Jing-Jing Wei,

Rui Hu

et al.

Biosensors and Bioelectronics, Journal Year: 2022, Volume and Issue: 215, P. 114552 - 114552

Published: July 9, 2022

Language: Английский

Citations

26
Ultrasensitive label-free electrochemical immunosensor of NT-proBNP biomarker based on branched AuPd nanocrystals/N-doped honeycombed porous carbon DOI

Chang Tang,

Jingxian Zhang,

Di-Nan Chen

et al.

Bioelectrochemistry, Journal Year: 2022, Volume and Issue: 148, P. 108225 - 108225

Published: Aug. 6, 2022

Language: Английский

Citations

25
Recent advances and future prospects of the potential-resolved strategy in ratiometric, multiplex, and multicolor electrochemiluminescence analysis DOI Creative Commons
Shijun Wang, Zhu Shu, Ziqi Kang

et al.

Theranostics, Journal Year: 2022, Volume and Issue: 12(15), P. 6779 - 6808

Published: Jan. 1, 2022

The potential-resolved strategy has gradually demonstrated its distinct values in electrochemiluminescence (ECL) bio-sensing due to superior characteristics, such as low instrument requirement, short assay time, and improved sample throughput, conjunction with spatial-and spectrum-resolved techniques.It recently been widely generalized into versatile multiple-signal ECL analytic platforms, especially ratiometric multiplex sensors, accordance some specific principles.Furthermore, luminophore pairs potential-and wavelength-resolved properties have utilized visualize biosensors that display multiple colors depending on analyte concentration.However, only a few comprehensive reports the principles, construction, application of various sensors schemes published.This review aims recount applying (a) (b) (c) multicolor discuss distinctions connections among principles these strategies.Finally, future prospects ECL-based analysis are explored.

Language: Английский

Citations

25
Dual-Mode Electrochemical Competitive Immunosensor Based on Cd2+/Au/Polydopamine/Ti3C2 Composite and Copper-Based Metal–Organic Framework for 17β-Estradiol Detection DOI

Yide Xia,

Yiwei Liu, Xiao Hu

et al.

ACS Sensors, Journal Year: 2022, Volume and Issue: 7(10), P. 3077 - 3084

Published: Oct. 5, 2022

Herein, a dual-mode electrochemical competitive immunosensor was constructed for the detection of 17β-estradiol (E2) based on differential pulse voltammetry (DPV) and chronoamperometry (i–t). During immune recognition process, E2 antibody (E2-Ab) immobilized Cd2+/Au/polydopamine/Ti3C2 (Cd2+/Au/pDA/Ti3C2) composite-modified electrode; then, E2-conjugated bovine serum albumin (E2-BSA) labeled with copper-based metal–organic framework (Cu-MOF) competed in combining E2-Ab. The Cu-MOF not only an electroactive species but also possessed good electrocatalytic activity toward H2O2. Thus, could be quantified according to peak current change DPV curve or variation H2O2 reduction current. For quantification, Cd2+ introduced as internal reference this case, highly reproducible ratio readout obtained. as-prepared showed linear relationship ranges 1 pg mL–1–10 ng mL–1 10 (i–t), limits were 0.47 5.4 (S/N = 3), respectively. Furthermore, exhibited practicability real sample analysis.

Language: Английский

Citations

24
Au Nanoparticle/CoFc/Metal–Organic Framework as Enzyme-Free Dual-Signal Ratiometric Electrochemical Sensor for In-Situ Determination of Cell-Secreted H2O2 DOI

Jiangbo Dong,

Jilin Zheng,

Jingzhou Hou

et al.

ACS Applied Nano Materials, Journal Year: 2023, Volume and Issue: 6(13), P. 11630 - 11639

Published: June 15, 2023

In-situ determination of cell-secreted biomolecules is important for elucidating its regulation signal transduction pathways, but detecting these low-level endogenous difficult. Herein, using H2O2 as the detection object, we developed an ultrasensitive enzyme-free ratiometric electrochemical sensing platform in a simple and low-cost method in-situ H2O2, which ferrocene (Fc) acts internal reference probe nanoflower Au-Co-MOF probe. First, flower-like CoFc-MOF was synthesized situ on flexible carbon cloth (CC) by one-step hydrothermal method, then Au-CoFc-MOF/CC obtained electrodeposition gold nanoparticles. The measurement results show that presence increases reduction peak Co-MOF (−0.32 V), while Fc (+0.4 V) almost unchanged. By taking full advantage obviously separated potential Co-MOF, well-designed can reflect concentration based IH2O2 – Iblank/IFc value 0.5 μM–10 mM range, with limit low 32 nM, ∼15.6 times lower than from single method. In addition, also demonstrates excellent selectivity, reproducibility, stability, biocompatibility. More significantly, this substrate shows great application provides feasible strategy other active substances released living cells clinical diagnosis.

Language: Английский

Citations

14