Aptamer-based CRISPR-Cas powered diagnostics of diverse biomarkers and small molecule targets

Applied Biological Chemistry, Journal Year: 2023, Volume and Issue: 66(1)

Published: Feb. 18, 2023

CRISPR-Cas systems have been widely used in genome editing and transcriptional regulation. Recently, effectors are adopted for biosensor construction due to its adjustable properties, such as simplicity of design, easy operation, collateral cleavage activity, high biocompatibility. Aptamers' excellent sensitivity, specificity, vitro synthesis, base-pairing, labeling, modification, programmability has made them an attractive molecular recognition element inclusion systems. Here, we review current advances aptamer-based sensors. We briefly discuss aptamers the knowledge Cas effector proteins, crRNA, reporter probes, analytes, applications target-specific aptamers. Next, provide fabrication strategies, binding, detection using fluorescence, electrochemical, colorimetric, nanomaterials, Rayleigh, Raman scattering. The application sensing a wide range biomarkers (disease pathogens) toxic contaminants is growing. This provides update offers novel insights into developing CRISPR-Cas-based sensors ssDNA with efficiency specificity point-of-care setting diagnostics.

Language: Английский

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Gold Nanomaterials‐Implemented CRISPR‐Cas Systems for Biosensing

Small, Journal Year: 2023, Volume and Issue: 19(21)

Published: Feb. 25, 2023

Due to their superiority in the simple design and precise targeting, clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have attracted significant interest for biosensing. On one hand, CRISPR-Cas capacity precisely recognize cleave specific DNA RNA sequences. other such as orthologs of Cas9, Cas12, Cas13 exhibit cis-cleavage or trans-cleavage activities after recognizing target sequence. Owing cleavage activities, can be designed biosensing by degrading tagged nucleic acids produce detectable signals. To meet requirements point-of-care detection versatile signal readouts, gold nanomaterials with excellent properties high extinction coefficients, easy surface functionalization, biocompatibility are implemented CRISPR-Cas-based biosensors. In combination nanoparticles, nanorods, nanostars, great efforts devoted fabricating biosensors diverse targets. This review focuses on current advances nanomaterials-implemented biosensors, particularly working mechanism performance these systems, including CRISPR-Cas9, CRISPR-Cas12a, CRISPR-Cas13a discussed highlighted. Meanwhile, prospects challenges also strategies based systems.

Language: Английский

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CRISPR-Cas assisted diagnostics: A broad application biosensing approach

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 162, P. 117028 - 117028

Published: March 18, 2023

In addition to its remarkable genome editing capability, the CRISPR-Cas system has proven be very effective in many fields of application, including biosensing pathogenic infections, mutagenic defects, or early cancer diagnosis. Thanks their advantages terms simplicity, efficiency, and reduced time, several systems have been described for design sensitive selective analytical tools, paving way development further commercialization next-generation diagnostics. However, CRISPR-Cas-based biosensors still need research efforts improve some drawbacks, such as target amplification, low reproducibility, lack knowledge exploited element robustness. This review aims describe latest trends technologies better highlight insights point out limitations that overcome future market entry medical

Language: Английский

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Ratio fluorescence/colorimetric dual mode aptasensor and smartphones-assisted miniaturized device for early diagnosis of prostate cancer

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 485, P. 150152 - 150152

Published: March 2, 2024

Language: Английский

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Ultrasensitive protein and exosome analysis based on a rolling circle amplification assisted-CRISPR/Cas12a strategy

Talanta, Journal Year: 2024, Volume and Issue: 273, P. 125906 - 125906

Published: March 11, 2024

Language: Английский

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Highly sensitive detection of aflatoxin B1 byCRISPR/Cas12a-assisted single nanoparticle counting

Food Chemistry, Journal Year: 2024, Volume and Issue: 443, P. 138557 - 138557

Published: Jan. 23, 2024

Language: Английский

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CRISPR/Cas systems for genomic Editing, biochemical Sensing, Bioanalysis, and diagnostics

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 112638 - 112638

Published: Jan. 1, 2025

Language: Английский

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CRISPR integrated biosensors: A new paradigm for cancer detection

Clinica Chimica Acta, Journal Year: 2025, Volume and Issue: 569, P. 120179 - 120179

Published: Feb. 1, 2025

Language: Английский

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Comprehensive Approaches to Expending CRISPR Biosensor Applications Beyond Nucleic Acid Targets

TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118179 - 118179

Published: Feb. 1, 2025

Language: Английский

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Recent trends and technical advancements in biosensors and their emerging applications in food and bioscience

Food Bioscience, Journal Year: 2022, Volume and Issue: 47, P. 101695 - 101695

Published: March 31, 2022

Language: Английский

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