Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: 441, P. 137964 - 137964
Published: May 14, 2025
Language: Английский
TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 173, P. 117640 - 117640
Published: March 8, 2024
Language: Английский
Citations
71
Journal of Pharmaceutical Analysis, Journal Year: 2024, Volume and Issue: 14(11), P. 100959 - 100959
Published: Feb. 28, 2024
An optical biosensor is a specialized analytical device that utilizes the principles of optics and light in bimolecular processes. Localized surface plasmon resonance (LSPR) phenomenon realm nanophotonics occurs when metallic nanoparticles (NPs) or nanostructures interact with incident light. Conversely, surface-enhanced Raman spectroscopy (SERS) an influential technique based on scattering, wherein it amplifies signals molecules they are situated near specific specially designed nanostructures. A detailed exploration recent ground-breaking developments biosensors employing LSPR SERS technologies has been thoroughly discussed along their underlying working mechanisms. chip created, featuring high-density deposition gold (AuNPs) under varying ligand concentration reaction duration substrate. ordinary description, visual illustration, provided for concepts such as sensogram, refractive index shift, (SPR), evanescent field, Rayleigh well electromagnetic enhancement chemical enhancement. both have advantages disadvantages, but widely used some over LSPR, like specificity, high sensitivity, multiplexing, versatility different fields. This review confirms elucidates significance disease biomarker identification. play vital role detection various types cancer, cervical ovarian endometrial prostate colorectal brain tumors. proposed offers potential applications early diagnosis monitoring viral disease, bacterial infectious diseases, fungal diabetes, cardiac biosensing. provide new direction environmental monitoring, food safety, refining impurities from water samples, lead detection. The understanding these still limited challenging.
Language: Английский
Citations
38
Food Chemistry, Journal Year: 2024, Volume and Issue: 447, P. 138663 - 138663
Published: Feb. 7, 2024
Language: Английский
Citations
26
International Journal of Microbiology, Journal Year: 2025, Volume and Issue: 2025(1)
Published: Jan. 1, 2025
Antimicrobial‐resistant Escherichia coli , especially those belonging to the serotype O157, are increasingly linked foodborne diseases with significant fatality rates worldwide. The food and medical industries have focused on E. O157:H7 due its ability produce toxins coupled low infectious dose. aim of this study was assess virulome, resistome, pathogenicity using whole genome sequencing. Three previously isolated strains from cattle feces were subjected sizes all three 5,117,276 bp, 5,039,443 5,034,351 bp. C + G contents 50.22%, 50.53%, 50.54%, while number contigs 110, 43, 42, respectively, for J32, J57, J69. Several virulence determinants (hemorrhagic pilus (HCP), eaeA, hemolysin, etc.) found in genomes these isolates. In addition, antibiotic resistance genes conferring aminoglycosides, tetracyclines, macrolides, fluoroquinolones, penams, carbapenems, cephalosporins, cephamycin, rifamycin, phenicols, monobactams, nitroimidazole genomes. Interestingly, isolates also harbored encoding disinfectants antiseptics, indicating their concern production sectors. This highlights public health concerns isolates, need constant surveillance.
Language: Английский
Citations
2
Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(11)
Published: March 10, 2025
Rapid and accurate detection of DNA from disease-causing pathogens is essential for controlling the spread infections administering timely treatments. While traditional molecular diagnostics techniques like PCR are highly sensitive, they include nucleic acid amplification many need to be performed in centralized laboratories, limiting their utility point-of-care settings. Recent advances CRISPR-based (CRISPR-Dx) have demonstrated potential specific detection, but sensitivity often constrained by slow trans-cleavage activity Cas enzymes, necessitating preamplification target acids. In this study, we present a CRISPR-Cascade assay that overcomes these limitations integrating positive feedback loop enables amplification-free pathogenic at atto-molar levels achieves signal-to-noise ratio greater than 1.3 within just 10 min. The versatility through bloodstream infection pathogens, including Methicillin-Sensitive Staphylococcus aureus (MSSA), Methicillin-Resistant (MRSA), Escherichia coli , Hepatitis B Virus (HBV) spiked whole blood samples. Additionally, introduce multiplexing OR-function logic gate, further enhancing rapid clinical Our findings highlight ability provide sensitive paving way advanced applications beyond.
Language: Английский
Citations
2
Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 228, P. 115189 - 115189
Published: March 2, 2023
Language: Английский
Citations
41
Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14
Published: Nov. 23, 2023
The World Health Organization (WHO) estimated that pathogens like Escherichia coli, primarily linked to food and water contamination, are associated with 485,000 deaths from diarrheal diseases annually, translating a staggering worldwide economic loss of nearly 12 billion USD per annum. International organizations the WHO United Nations Children’s Fund (UNICEF) have established related guidelines criteria for pathogenic detection technologies driving search innovative efficient methods. This comprehensive review examines trajectory waterborne bacteria traditional techniques, i.e., culture-based methods, current methods including various forms polymerase chain reaction (PCR) techniques [qualitative real-time PCR, digital ELISA, loop-mediated isothermal amplification, next-generation sequencing (NGS)] emerging biosensors artificial intelligence (AI). scope paper focuses on recognized as human pathogens, posing tangible threats public health through waterborne. techniques’ merits, constraints, research gaps future perspectives critically discussed. Advancements in droplet NGS significantly improved sensitivity specificity, revolutionizing pathogen detection. Additionally, integration (AI) these has enhanced accuracy, enabling analysis large datasets. Molecular-based show promise quality monitoring, especially resource-constrained settings, but on-site practical implementation remains challenge. pairwise comparison metrics used this also offer valuable insights into quick evaluation advantages, limitations focusing their applicability field settings timely analyses. Future efforts should focus developing robust, cost-effective user-friendly routine ultimately safeguarding global supplies health, AI data playing crucial role advancing safer environment.
Language: Английский
Citations
38
Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 457, P. 131823 - 131823
Published: June 10, 2023
Language: Английский
Citations
29
TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 176, P. 117775 - 117775
Published: May 20, 2024
Language: Английский
Citations
11
Computers and Electronics in Agriculture, Journal Year: 2025, Volume and Issue: 229, P. 109941 - 109941
Published: Jan. 20, 2025
Citations
1