Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 27, 2025
Metal-organic
framework-based
hybrids
(MOFzyme)
have
promising
applications
in
colorimetric
aptasensors
due
to
their
highly
efficient
and
stable
catalytic
activity.
However,
application
biosensors
remains
a
challenging
issue
the
limited
reaction
site
amorphous
structure.
Herein,
we
encapsulated
catalase
inside
MOF
cavities
prepare
an
MOFzyme
with
many
functional
groups
on
its
surface,
were
utilized
for
subsequent
integration
of
into
hyaluronic
acid-DNA
hydrogel.
Moreover,
accurate
flexible
synthesis
unit
hydrogel
enabled
prepared
DNA
adjust
shape
suit
encapsulate
MOFzyme.
Subsequently,
novel
sensing
strategy
detecting
Sarafloxacin
(Sar)
was
developed
based
excellent
ability
mediated
by
stimulus
responsiveness
In
addition,
evolved
aptamer
higher
affinity
Sar
obtained
via
semirational
design
elaborately
designed
synthetic
hydrogel,
significantly
improving
response
sensitivity
specificity
aptasensor.
The
constructed
aptasensor
exhibited
wide
linear
detection
range
0.003
200
ng/mL
low
limit
2.06
pg/mL.
Above
all,
this
work
provides
sensitive
way
determine
hazardous
substances
food
environments.
Frontiers in Bioscience-Elite,
Journal Year:
2024,
Volume and Issue:
16(1), P. 4 - 4
Published: Jan. 31, 2024
Elevated
concentrations
of
toxic
organic
compounds
observed
in
food
products
pose
serious
dangers
to
human
health.
Both
natural
and
artificial
pollutants
can
cause
contamination.
The
stages
production,
packaging,
transportation,
storage
also
largely
the
appearance
undesirable
substances
products.
health
consequences
ingesting
containing
contaminants
range
from
mild
gastroenteritis
deaths
resulting
dysfunctional
internal
organs
neurological
syndromes.
World
Health
Organization
(WHO)
sets
recommendations
for
content
such
chemicals
food,
including
a
minimum
allowable
concentration
considered
safe
consumption.
However,
control
chemical
is
necessary.
Moreover,
fast,
sensitive,
inexpensive
methods
are
needed
detect
them
at
point
need.
Currently,
immune
analysis
most
widely
used
determine
food.
development
fluorescence
polarization
immunoassay
(FPIA)
competitive
format
powerful
modern
tool
detecting
molecules
various
matrices,
thereby
making
FPIA
useful
safety
applications.
Due
availability
portable
devices
measuring
signal,
be
variety
fluorescent
labels
recognizing
elements
(receptors,
monoclonal
polyclonal
antibodies,
nanobodies)
permits
(FP)
assays
significantly
lower
limits
substances.
FP
assay
homogeneous,
quantitative
method.
formats
makes
promising
determining
pollutants.
This
review
summarizes
publications
on
analyses
(pesticides,
hormones,
toxins,
antibiotics,
other
pharmaceuticals)
during
2018–2023.
Further,
it
demonstrates
prospects
using
this
method
need
high
molecular
weight
substances,
fungi,
bacterial
infections
inspections.
