CRISPR/Cas9-mediated SERS/colorimetric dual-mode lateral flow platform combined with smartphone for rapid and sensitive detection of Staphylococcus aureus

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 249, P. 116046 - 116046

Published: Jan. 17, 2024

Language: Английский

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Development of a Colorimetric and SERS Dual-Signal Platform via dCas9-Mediated Chain Assembly of Bifunctional Au@Pt Nanozymes for Ultrasensitive and Robust Salmonella Assay

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(31), P. 12684 - 12691

Published: July 22, 2024

Timely screening for harmful pathogens is a great challenge in emergencies where traditional culture methods suffer from long assay time and alternative are limited by poor accuracy low robustness. Herein, we present dCas9-mediated colorimetric surface-enhanced Raman scattering (SERS) dual-signal platform (dCas9-CSD) to address this challenge. Strategically, the used dCas9 accurately recognize repetitive sequences amplicons produced loop-mediated isothermal amplification (LAMP), forming nucleic acid frameworks that assemble numerous bifunctional gold-platinum (Au@Pt) nanozymes into chains on surface of streptavidin-magnetic beads (SA-MB). The collected Au@Pt converted colorless 3,3',5,5'-tetramethylbenzidine (TMB) blue oxidized TMB (oxTMB) via its Pt shell then enhanced signal oxTMB Au core. Therefore, presence

Language: Английский

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Isothermal nucleic acid amplification-based biosensors: The next generation analytical toolkit for point-of-care assay of foodborne pathogens

Trends in Food Science & Technology, Journal Year: 2025, Volume and Issue: unknown, P. 104882 - 104882

Published: Jan. 1, 2025

Language: Английский

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Gold nanoparticle–based lateral-flow immunochromatographic biosensing assays for the diagnosis of infections

Biosensors and Bioelectronics X, Journal Year: 2024, Volume and Issue: 17, P. 100457 - 100457

Published: March 6, 2024

Infections cause concern for mankind, and sometimes their diagnosis may take much time. Owing to its high sensitivity, rapidity, specificity, the immunochromatographic assay (ICA) is used widely diagnose infections. The use of ICA this purpose makes it possible detect dangerous diseases early, prevent progress, strongly reduce treatment cost mortality, increase life expectancy. One promising format involves gold nanoparticles. Here we present principles history infections discuss prospects using nanoparticles in ICA. We data on methods make conjugate effect particle size shape sensitivity. bacterial viral infections, review instrumental quantifying results. Finally, nanoparticle–based

Language: Английский

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A Label-Free and Naked-Eye Fluorescence Turn-On Assay for One-Pot Lamp Detection of Foodborne Pathogens Using Auncs-Cu2+ Complex

Published: Jan. 1, 2025

Language: Английский

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(Co, S)-Codoped BiOBr as a novel signal probe coupled with LAMP (H+)-pH responsive photoelectrochemistry biosensor for ultrasensitive detection of microRNA

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 422, P. 136482 - 136482

Published: Aug. 22, 2024

Language: Английский

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CRISPR/dCas9-based hotspot self-assembling SERS biosensor integrated with a smartphone for simultaneous, ultrasensitive and robust detection of multiple pathogens

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 270, P. 116974 - 116974

Published: Nov. 19, 2024

Language: Английский

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An integrated self-heating recombinase polymerase amplification lateral flow strip biosensor for quantification of enterohemorrhagic Escherichia coli O157:H7

Microchemical Journal, Journal Year: 2024, Volume and Issue: 199, P. 109979 - 109979

Published: Jan. 14, 2024

Language: Английский

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A Lateral Flow Biosensor Based on Isothermal Amplification for Visual Identification of Species and Sex from Bloodstain

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: July 20, 2024

The prompt species identification from biological samples at a crime scene can rapidly filter out truly valuable biometric information for subsequent personal identification. Meanwhile, early sex determination assist in narrowing the pool of suspects. However, current methods forensic DNA analysis, particularly point-of-care scenarios, are often limited by intricate equipment signal generation and laborious procedure purification. present study introduces novel portable lateral flow biosensor that possesses extraction-free anti-aerosol characteristics on-site sex. bloodstain be directly submitted to loop-mediated isothermal amplification (LAMP) analysis both mitochondrial nuclear DNA. incorporation device with gold magnetic nanoparticle probes allows visual interpretation results through colorimetric signals while also preventing interference on result judgment pigments such as hemoglobin. Carryover contamination, which is disharmonious factor LAMP, especially inherent contradiction derived uncapping strategy, has been effectively addressed integration uracil glycosylase without compromising isothermy throughout process. As proof-of-concept experiment, accurately identified within 40 min trace bloodstains amidst significant background targeting cytochrome

Language: Английский

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A multi-channel microfluidic chip based on fluorescent distance readout-mode for rapid, simultaneous and visual detection of multiplex pathogens using phage- AIEgen-antimicrobial peptide-encoded tags

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: unknown, P. 136867 - 136867

Published: Oct. 1, 2024

Language: Английский

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