Drop‐by‐Drop Addition of Reagents to a Double Emulsion DOI Creative Commons
Thomas W. Cowell, Wenyang Jing,

Heewon Noh

et al.

Small, Journal Year: 2024, Volume and Issue: 20(46)

Published: Aug. 5, 2024

Developments in droplet microfluidics have facilitated an era of high-throughput, sensitive single-cell, or single-molecule measurements capable tackling the heterogeneity present biological systems. Relying on single emulsion (SE) compartments, assays achieve absolute quantification nucleic acids, massively parallel single-cell profiling, and more. Double emulsions (DEs) seen recent interest for their potential to build upon SE techniques. DEs are compatible with flow cytometry enabling high-throughput multi-parameter drop screening eliminate content mixing due coalescence during lengthy workflows. Despite these strengths, lack important technical functions that exist SEs such as methods adding reagents droplets demand. Consequently, cannot be used multistep workflows which has limited adoption assay development. Here, strategies enable reagent addition other active manipulations reported by converting DE inputs chip. After conversion, drops manipulated using existing techniques, including addition, before reforming a at outlet. Device designs operation conditions achieving drop-by-drop identified part multi-step aptamer performed entirely drops. This work enables further development assays.

Language: Английский

Rapid filamentous fungi gene knockout identification through high-throughput droplet microfluidics DOI
Yuwen Li, Jing Dai, Huan Zhang

et al.

Analytica Chimica Acta, Journal Year: 2025, Volume and Issue: 1358, P. 344094 - 344094

Published: April 18, 2025

Language: Английский

Citations

0
Drop‐by‐Drop Addition of Reagents to a Double Emulsion DOI Creative Commons
Thomas W. Cowell, Wenyang Jing,

Heewon Noh

et al.

Small, Journal Year: 2024, Volume and Issue: 20(46)

Published: Aug. 5, 2024

Developments in droplet microfluidics have facilitated an era of high-throughput, sensitive single-cell, or single-molecule measurements capable tackling the heterogeneity present biological systems. Relying on single emulsion (SE) compartments, assays achieve absolute quantification nucleic acids, massively parallel single-cell profiling, and more. Double emulsions (DEs) seen recent interest for their potential to build upon SE techniques. DEs are compatible with flow cytometry enabling high-throughput multi-parameter drop screening eliminate content mixing due coalescence during lengthy workflows. Despite these strengths, lack important technical functions that exist SEs such as methods adding reagents droplets demand. Consequently, cannot be used multistep workflows which has limited adoption assay development. Here, strategies enable reagent addition other active manipulations reported by converting DE inputs chip. After conversion, drops manipulated using existing techniques, including addition, before reforming a at outlet. Device designs operation conditions achieving drop-by-drop identified part multi-step aptamer performed entirely drops. This work enables further development assays.

Language: Английский

Citations

0