Advances of nanobody-based immunosensors for detecting food contaminants

Trends in Food Science & Technology, Journal Year: 2025, Volume and Issue: unknown, P. 104871 - 104871

Published: Jan. 1, 2025

Language: Английский

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Structural Insights Into the Mechanisms of Recognition of Recombinant Full-Length Antibodies for the Detection of Staphylococcus aureus Enterotoxins C1, C2, and C3

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Food poisoning caused by Staphylococcus aureus (S. aureus) and its enterotoxins has become a global food safety issue. Herein, three types of Staphylococcal (SE), including SEC1, SEC2, SEC3, were successfully expressed immunized in mice to prepare monoclonal antibodies (mAbs). We screened pair mAbs 16E12-9B7 from 12 strains that could simultaneously recognize SEC3. Furthermore, the genes hybridoma cells 9B7 16E12 extracted, amplified, inserted into expression vectors obtain recombinant (rAbs), whose affinities consistent with those ascites antibodies. The paired rAbs applied gold immunochromatographic strip (GICS) system enable rapid detection SEC3 visual limits (vLOD) 2, 0.5, 2 ng/mL milk samples. Noticeably, we found hydrogen bonds salt bridges played significant role these antigen–antibody interactions. key sites for ASN28 SER31 complementarity determining region (CDR) SER32 variable light chain (VL) ARG100, SER101, TYR102 heavy (VH). analysis potential screening mutant

Language: Английский

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Nanobody-Based Immunoassays for the Detection of Food Hazards—A Review

Biosensors, Journal Year: 2025, Volume and Issue: 15(3), P. 183 - 183

Published: March 13, 2025

Food safety remains a significant global challenge that affects human health. Various hazards, including microbiological and chemical threats, can compromise food throughout the supply chain. To address issues ensure public health, it is necessary to adopt rapid, accurate, highly specific detection methods. Immunoassays are considered be an effective method for of sensitive biochemical indicators provide efficient platform identification hazards. In immunoassays, antibodies function as primary recognition elements. Nanobodies have potential valuable biomolecules in diagnostic applications. Their distinctive physicochemical structural characteristics make them excellent candidates development reliable assays, promising alternatives monoclonal polyclonal antibodies. Herein, we summarize comprehensive overview status prospects nanobody-based immunoassays ensuring safety. First, begin with historical perspective on nanobodies their unique characteristics. Subsequently, explore definitions boundaries immunosensors, before discussing applications testing emerged over past five years, follow different highlighting advantages traditional Finally, directions challenges discussed. Due remarkable sensitivity, specificity versatility, hold great promise revolutionizing health well-being.

Language: Английский

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Bioelectronics based on antibody-conjugated graphene field-effect transistor for response of Staphylococcal enterotoxin B as a biological weapon

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 494, P. 138457 - 138457

Published: May 2, 2025

Language: Английский

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Magnetic-plasmonic blackbody enhanced lateral flow immunoassay of staphylococcal enterotoxin B

Food Chemistry, Journal Year: 2024, Volume and Issue: 465, P. 142130 - 142130

Published: Nov. 19, 2024

Language: Английский

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State-of-the-Art Signal Amplification Strategies for Nucleic Acid and Non-Nucleic Acid Biosensors

Sensors and Actuators Reports, Journal Year: 2024, Volume and Issue: unknown, P. 100268 - 100268

Published: Dec. 1, 2024

Language: Английский

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