Rapid Identification of Cell Types and Phenotypic States Using a One-Polymer Multichannel Nanosensor Fabricated via Flash Nanoprecipitation DOI
Mingdi Jiang,

Junwhee Yang,

Liang Liu

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 9, 2024

Cell state transitions are fundamental in biology, determining how cells respond to environmental stimuli and adapt diseases treatments. surface-based sensing of geno/phenotypes is a versatile approach for distinguishing different cell types states. Array-based biosensors can provide highly sensitive platform based on the differential interactions each element with surface components. In this work, modular polymer-based supramolecular multichannel sensor array (FNP sensor) was fabricated by encapsulating hydrophobic dye (pyrene) into monolayer positively charged fluorescent polymer through flash nanoprecipitation (FNP). We utilized one-polymer discriminate among commonly found tumors: 4T1 cancer cells, NIH/3T3 fibroblast RAW 264.7 macrophage cells. The also successfully characterized varying ratios cancer-associated fibroblasts (CAFs) tumor-associated macrophages (TAMs). This single provides effective discrimination high reproducibility, providing high-throughput tool diagnostic screening states associated progression.

Language: Английский

Multichannel Wearable Sensor Based on Porous Structure for Simultaneous Acquisition of Different Mechanical Signals DOI
Lei Wen, Haizhou Huang, Yuheng Huang

et al.

ACS Applied Electronic Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

Language: Английский

Citations

0
Rapid Identification of Cell Types and Phenotypic States Using a One-Polymer Multichannel Nanosensor Fabricated via Flash Nanoprecipitation DOI
Mingdi Jiang,

Junwhee Yang,

Liang Liu

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 9, 2024

Cell state transitions are fundamental in biology, determining how cells respond to environmental stimuli and adapt diseases treatments. surface-based sensing of geno/phenotypes is a versatile approach for distinguishing different cell types states. Array-based biosensors can provide highly sensitive platform based on the differential interactions each element with surface components. In this work, modular polymer-based supramolecular multichannel sensor array (FNP sensor) was fabricated by encapsulating hydrophobic dye (pyrene) into monolayer positively charged fluorescent polymer through flash nanoprecipitation (FNP). We utilized one-polymer discriminate among commonly found tumors: 4T1 cancer cells, NIH/3T3 fibroblast RAW 264.7 macrophage cells. The also successfully characterized varying ratios cancer-associated fibroblasts (CAFs) tumor-associated macrophages (TAMs). This single provides effective discrimination high reproducibility, providing high-throughput tool diagnostic screening states associated progression.

Language: Английский

Citations

0