Ultrasensitive COFs Functionalized Electrochemical Biosensor for DNA Methyltransferase Activity Detection by DNA Walking and Rolled Circular Strand Displacement Amplification

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 11, 2025

Assessing the activity of DNA methyltransferases (MTases) and screening for methyltransferase inhibitors not only allow a deep exploration role methylation regulation in disease initiation progression but also provide an important experimental clinical basis diagnosis treatment diseases. Herein, new COFs functionalized electrochemical biosensor has been developed to detect adenine (Dam) MTase with high sensitivity rapidity by taking advantage walker rolled circular strand displacement amplification (RC-SDA) reaction. Specifically, hairpin probe H1 was methylated Dam MTase, followed site-specific cleavage DpnI enzyme generate S5 probe. The padlock probes two nucleic acid endonuclease sites were introduced trigger RC-SDA reaction under action primer, releasing large number single-stranded S6 probes. released worked synergistically Exo III walking, which exposed binding enable Au-COF-MB signal bind effectively electrode surface, signals thus generated. result showed that designed demonstrated excellent specificity detecting detection limit (LOD) 6.85 × 10-5 U/mL. This method provides support development strategies related

Language: Английский

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Signal-amplifying biosensors for miRNA detection and their extension in portable glucometer

Talanta, Journal Year: 2025, Volume and Issue: 288, P. 127714 - 127714

Published: Feb. 13, 2025

Language: Английский

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Recent Advancements in Hybrid Electrochemical Biosensors Nanoarchitectronics

Hybrid Advances, Journal Year: 2024, Volume and Issue: 8, P. 100347 - 100347

Published: Dec. 3, 2024

Language: Английский

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