Electrochemical Reactions Affected by Electric Double Layer Overlap in Conducting Nanopores DOI

Hyo Chan Lee,

Jinju Kim,

Hyun Ju Yang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(47), P. 18745 - 18753

Published: Nov. 15, 2024

When a potential is applied to an electrode immersed in electrolyte solution, ions with opposite charges accumulate around the electrode, forming electrical double layer (EDL). Unlike flat electrodes, nanoporous electrodes pore sizes comparable EDL thickness experience overlapping EDLs, altering electrochemically effective surface area. Although previous research has primarily examined ion charging dynamics and formation impact of overlap on Faraday reactions remains underexplored. In this study, we influence electrochemical within using chronoamperometry DC AC voltammetry. We used concentration, measurement duration, overpotential, material as variables determine relationship between extent reaction. The concentration-dependent reaction due was more pronounced for faster changes, shorter times, lower overpotentials, slower catalytic activity. This unique phenomenon that not observed electrodes. These findings provide insight into utilization sensor applications.

Language: Английский

Surface Charge Effects of Monovalent and Zwitterionic Monolayers to Differentiate Structurally Similar Aminoglycosides with Electrochemical Aptamer Biosensors DOI

Michael J. McGowan,

Andrew Trowbridge, Julius Reitemeier

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 276, P. 117229 - 117229

Published: Feb. 7, 2025

Language: Английский

Citations

1
Target-Triggered DNA Hydrogel Gating with Nanoconfinement Effect in Micropipettes for Antibiotic Resistance Gene Detection DOI

Xueyan Zhou,

Juan Song, Qi Zhu

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 16, 2025

A novel biosensing platform integrating molecular gating mechanisms with confined-space amplification is presented for ultrasensitive nucleic acid detection. The employs a target-triggered clamped hybridization chain reaction (C-HCR) to form DNA hydrogel networks in gold-modified glass micropipettes, creating nanoconfined spaces. Notably, this configuration demonstrates effective ion enhanced sensitivity through the nanoconfinement effect as modulation of current rectification signals significantly amplified. Moreover, can be regenerated thermal treatment, endowing device good recoverability. performance was validated by using different antibiotic resistance genes model targets. results showed high specificity and limits detection down subnanomolar levels. confined environment enables sensitive small sample volumes only 4 μL. strategy leveraging amplify signal transduction may provide inspiration rational design other high-performance biosensors, potentially holding great promise disease diagnostics environmental monitoring.

Language: Английский

Citations

0
Monitoring Populations of Single Extracellular Vesicles from Pseudomonas aeruginosa Using Large Parallel Arrays of Zero-Mode Waveguides DOI Creative Commons
Jarek Metro, Abigail A. Weaver, Julius Reitemeier

et al.

Precision Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

Extracellular vesicles (EV) have emerged as key factors for intercellular communication, disease biomarkers, and vaccines, but EV populations generally exhibit broad heterogeneity, making single-vesicle measurements critical in order to understand the roles played by EVs pathways they utilize. To circumvent exhaustive isolation concentration protocols and/or long incubation periods required common characterization methods, we developed a method situ study of single from crude Pseudomonas aeruginosa culture real-time with minimal sample preparation using nanopore-based zero-mode waveguides (ZMW). The dimensions ZMW allow only occupy nanopore volume, it possible monitor large arrays one-at-a-time parallel. Furthermore, attoliter-volume nanopores restrict much larger P. cells entering observation eliminating need isolate their parent cells. Lipophilic fluorophores are used selectively tag membrane, thereby restricting optical observations captured individual nanopores. By fashioning ZMWs into 21 × arrays, 441 volumes can be observed parallel, revealing heterogeneity responses, which is usually masked ensemble averaging when examining hundreds events at once without spatial segregation. ability enables analysis changes bacterial environment, since detection governed solely diffusion particle volume. work described here presents an approach studying makes observe shifts vesicle population response perturbations real-time.

Language: Английский

Citations

0
Nanoconfined constructs for electrochemical aptamer-based in vivo biosensing DOI Creative Commons

Grayson F. Huldin,

Junming Huang, Kaiyu Fu

et al.

Current Opinion in Electrochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 101695 - 101695

Published: April 1, 2025

Language: Английский

Citations

0
Single-Capsid Identification of Full and Empty Status of Recombinant Adeno-Associated Viruses via Resistive Pulse Sensing DOI Creative Commons

Hanna T. Nguyen,

Farhad Shiri,

Matthew Verber

et al.

Sensors and Actuators Reports, Journal Year: 2024, Volume and Issue: 8, P. 100242 - 100242

Published: Sept. 6, 2024

Language: Английский

Citations

1
Electrochemical Reactions Affected by Electric Double Layer Overlap in Conducting Nanopores DOI

Hyo Chan Lee,

Jinju Kim,

Hyun Ju Yang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(47), P. 18745 - 18753

Published: Nov. 15, 2024

When a potential is applied to an electrode immersed in electrolyte solution, ions with opposite charges accumulate around the electrode, forming electrical double layer (EDL). Unlike flat electrodes, nanoporous electrodes pore sizes comparable EDL thickness experience overlapping EDLs, altering electrochemically effective surface area. Although previous research has primarily examined ion charging dynamics and formation impact of overlap on Faraday reactions remains underexplored. In this study, we influence electrochemical within using chronoamperometry DC AC voltammetry. We used concentration, measurement duration, overpotential, material as variables determine relationship between extent reaction. The concentration-dependent reaction due was more pronounced for faster changes, shorter times, lower overpotentials, slower catalytic activity. This unique phenomenon that not observed electrodes. These findings provide insight into utilization sensor applications.

Language: Английский

Citations

0