Customizable and Interactive Visualizations for Investigating Spatio-Temporal Single-Cell Information DOI
Johannes Seiffarth, Luisa Blöbaum, Alexander Grünberger

et al.

Published: May 27, 2024

Language: Английский

Insights into the morphology‐productivity relationship of filamentous fungi through small‐scale cultivation and automated microscopy of Thermothelomyces thermophilus DOI Creative Commons

Katja Rohr,

Bertram Geinitz, Johannes Seiffarth

et al.

Biotechnology Progress, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Abstract Filamentous fungi are a cornerstone in the biotechnological production of enzymes, proteins, and organic acids. However, challenges understanding controlling relationship between morphology productivity can limit their application. This study addresses these using Thermothelomyces thermophilus , promising thermophilic fungus known for thermostable enzymes. We investigated effects environmental conditions on fungal enzyme combination microbioreactor cultivation, automated liquid handling, microscopy. Specifically, batch fed cultivations were performed at different pH levels glucose feeding rates to secretory phytase production, growth, morphology. Results from revealed two‐fold higher activity 5.5 compared 6.5, with notably smaller fragments end cultivation. Conversely, rate 1 g (l h) −1 showed 1.6‐fold 5.5, accompanied by much larger aggregates throughout phase. These findings suggest that large associated high productivity; however, breakdown further enhances release, increasing supernatant. not only provides insights morphology‐productivity T. but also demonstrates efficacy integrating microbioreactors methodology represents significant advance field biotechnology, paving way more efficient industrial bioprocesses.

Language: Английский

Citations

0
Unveiling microbial single-cell growth dynamics under rapid periodic oxygen oscillations DOI Creative Commons
Keitaro Kasahara, Johannes Seiffarth, Birgit Stute

et al.

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Time-resolved microbial growth analyses at single-cell resolution under temporally controlled O 2 environments.

Language: Английский

Citations

0
Tracking One-in-a-Million: Large-Scale Benchmark for Microbial Single-Cell Tracking with Experiment-Aware Robustness Metrics DOI Creative Commons
Johannes Seiffarth, Luisa Blöbaum, Richard Dominik Paul

et al.

Lecture notes in computer science, Journal Year: 2025, Volume and Issue: unknown, P. 318 - 334

Published: Jan. 1, 2025

Language: Английский

Citations

0
BIOMERO: A scalable and extensible image analysis framework DOI Creative Commons
Torec T. Luik,

Rodrigo Rosas-Bertolini,

Eric A. Reits

et al.

Patterns, Journal Year: 2024, Volume and Issue: 5(8), P. 101024 - 101024

Published: July 18, 2024

Language: Английский

Citations

0
A microfluidic system for the cultivation of cyanobacteria with precise light intensity and CO2 control: Enabling growth data acquisition at single-cell resolution. DOI Creative Commons

Lennart Witting,

Johannes Seiffarth, Birgit Stute

et al.

Lab on a Chip, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

A versatile microfluidic platform with precise multi-parameter control for high-throughput cyanobacterial growth analysis at single-cell resolution.

Language: Английский

Citations

0
Robust Approximate Characterization of Single-Cell Heterogeneity in Microbial Growth DOI

Richard D. Paul,

Johannes Seiffarth, Hanno Scharr

et al.

Published: May 27, 2024

Language: Английский

Citations

0
Customizable and Interactive Visualizations for Investigating Spatio-Temporal Single-Cell Information DOI
Johannes Seiffarth, Luisa Blöbaum, Alexander Grünberger

et al.

Published: May 27, 2024

Language: Английский

Citations

0