Nature Medicine,
Journal Year:
2024,
Volume and Issue:
30(10), P. 2947 - 2956
Published: July 31, 2024
The
ecosystem
of
brain
tumors
is
considered
immunosuppressed,
but
our
current
knowledge
may
be
incomplete.
Here
we
analyzed
clinical
cell
and
tissue
specimens
derived
from
patients
presenting
with
glioblastoma
or
nonmalignant
intracranial
disease
to
report
that
the
cranial
bone
(CB)
marrow,
in
juxtaposition
treatment-naive
tumors,
harbors
active
lymphoid
populations
at
time
initial
diagnosis.
Clinical
anatomical
imaging,
single-cell
molecular
immune
profiling
quantification
tumor
reactivity
identified
CD8
Nature,
Journal Year:
2021,
Volume and Issue:
601(7894), P. 600 - 605
Published: Dec. 2, 2021
Abstract
One
week
after
fertilization,
human
embryos
implant
into
the
uterus.
This
event
requires
embryo
to
form
a
blastocyst
consisting
of
sphere
encircling
cavity
lodging
proper.
Stem
cells
can
model
that
we
called
blastoid
1
.
Here
show
naive
pluripotent
stem
cultured
in
PXGL
medium
2
and
triply
inhibited
for
Hippo,
TGF-β
ERK
pathways
efficiently
(with
more
than
70%
efficiency)
blastoids
generating
blastocyst-stage
analogues
three
founding
lineages
(more
97%
trophectoderm,
epiblast
primitive
endoderm)
according
sequence
timing
development.
Blastoids
spontaneously
first
axis,
observe
induces
local
maturation
polar
thereby
endowing
with
capacity
directionally
attach
hormonally
stimulated
endometrial
cells,
as
during
implantation.
Thus,
propose
such
is
faithful,
scalable
ethical
investigating
implantation
development
3,4
Nature,
Journal Year:
2023,
Volume and Issue:
unknown
Published: Sept. 6, 2023
The
ability
to
study
human
post-implantation
development
remains
limited
owing
ethical
and
technical
challenges
associated
with
intrauterine
after
implantation1.
Embryo-like
models
spatially
organized
morphogenesis
structure
of
all
defining
embryonic
extra-embryonic
tissues
the
conceptus
(that
is,
disc,
bilaminar
yolk
sac,
chorionic
sac
surrounding
trophoblast
layer)
remain
lacking1,2.
Mouse
naive
stem
cells
have
recently
been
shown
give
rise
capable
self-assembling
into
post-gastrulation
structured
stem-cell-based
embryo
(called
SEMs)3.
Here
we
extend
those
findings
humans
using
only
genetically
unmodified
(cultured
in
enhanced
cell
medium
conditions)4.
Such
fully
integrated
complete
SEMs
recapitulate
organization
nearly
known
lineages
compartments
embryos,
including
epiblast,
hypoblast,
mesoderm
layer
latter
compartments.
These
demonstrated
developmental
growth
dynamics
that
resemble
key
hallmarks
stage
embryogenesis
up
13-14
days
fertilization
(Carnegie
6a).
include
disc
formation,
epiblast
lumenogenesis,
polarized
amniogenesis,
anterior-posterior
symmetry
breaking,
primordial
germ-cell
specification,
visceral
parietal
endoderm
expansion
defines
a
cavity
connecting
stalk,
trophoblast-surrounding
compartment
demonstrating
syncytium
lacunae
formation.
This
SEM
platform
will
probably
enable
experimental
investigation
previously
inaccessible
windows
early
post
implantation
peri-gastrulation
development.
Cell stem cell,
Journal Year:
2023,
Volume and Issue:
30(9), P. 1148 - 1165.e7
Published: Sept. 1, 2023
Naive
human
pluripotent
stem
cells
have
the
remarkable
ability
to
self-organize
into
blastocyst-like
structures
("blastoids")
that
model
lineage
segregation
in
pre-implantation
embryo.
However,
extent
which
blastoids
can
recapitulate
defining
features
of
post-implantation
development
remains
unexplored.
Here,
we
report
cultured
on
thick
three-dimensional
(3D)
extracellular
matrices
capture
hallmarks
early
development,
including
epiblast
lumenogenesis,
rapid
expansion
and
diversification
trophoblast
lineages,
robust
invasion
extravillous
by
day
14.
Extended
blastoid
culture
results
localized
activation
primitive
streak
marker
TBXT
emergence
embryonic
germ
layers
21.
We
also
show
modulation
WNT
signaling
alters
balance
between
fates
blastoids.
This
work
demonstrates
3D-cultured
offer
a
continuous
integrated
vitro
system
extraembryonic
from
gastrulation
stages.
Development,
Journal Year:
2023,
Volume and Issue:
150(10)
Published: May 15, 2023
Embryo
implantation
in
humans
is
interstitial,
meaning
the
entire
conceptus
embeds
endometrium
before
placental
trophoblast
invades
beyond
uterine
mucosa
into
underlying
inner
myometrium.
Once
implanted,
embryo
survival
pivots
on
transformation
of
an
anti-inflammatory
bed,
termed
decidua,
under
homeostatic
control
natural
killer
cells.
Here,
we
examine
evolutionary
context
and
elaborate
remodelling
after
conception
humans.
We
also
discuss
interactions
between
decidualising
that
regulate
interstitial
determine
fitness.
Together,
this
Review
highlights
precarious
but
adaptable
nature
process.
Cell stem cell,
Journal Year:
2023,
Volume and Issue:
30(12), P. 1569 - 1584
Published: Oct. 18, 2023
Studies
of
mammalian
development
have
advanced
our
understanding
the
genetic,
epigenetic,
and
cellular
processes
that
orchestrate
embryogenesis
uncovered
new
insights
into
unique
aspects
human
embryogenesis.
Recent
studies
now
produced
first
epigenetic
maps
early
embryogenesis,
stimulating
ideas
about
reprogramming,
cell
fate
control,
potential
mechanisms
underpinning
developmental
plasticity
in
embryos.
In
this
review,
we
discuss
these
regulation
importance
for
safeguarding
development.
We
also
highlight
unanswered
questions
key
challenges
remain
to
be
addressed.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2021,
Volume and Issue:
unknown
Published: May 7, 2021
Summary
Stem
cell-based
embryo
models
offer
unprecedented
experimental
tools
for
studying
early
human
development.
The
usefulness
of
hinges
on
their
molecular,
cellular
and
structural
fidelities
to
in
vivo
counterparts.
To
authenticate
models,
single-cell
RNA-sequencing
has
been
utilised
unbiased
transcriptional
profiling.
However,
a
well-organised
integrated
dataset,
serving
as
universal
reference
benchmarking
remains
unavailable.
Herein,
we
developed
such
reference,
through
integration
six
published
datasets
covering
developmental
stages
from
the
zygote
gastrula.
Lineage
annotations
are
contrasted
validated
with
available
non-human
primate
datasets.
Using
stabilised
UMAP
constructed
web
tool,
where
query
can
be
projected
annotated
predicted
cell
identities.
this
examined
several
recent
highlighting
risk
misannotation
when
relevant
references
lacking.
