Intravital microscopy of satellite cell dynamics and their interaction with myeloid cells during skeletal muscle regeneration DOI Creative Commons
Yingzhu He, Youshan Heng, Zhongya Qin

et al.

Science Advances, Journal Year: 2023, Volume and Issue: 9(42)

Published: Oct. 18, 2023

Skeletal muscle regeneration requires the highly coordinated cooperation of satellite cells (MuSCs) with other cellular components. Upon injury, myeloid populate wound site, concomitant MuSC activation. However, detailed analysis MuSC-myeloid cell interaction is hindered by lack suitable live animal imaging technology. Here, we developed a dual-laser multimodal nonlinear optical microscope platform to study dynamics MuSCs and their nonmyogenic during regeneration. Using three-dimensional time-lapse on reporter mice taking advantages autofluorescence reduced nicotinamide adenine dinucleotide (NADH), studied spatiotemporal between stem/progenitor activation proliferation. We discovered that cell-cell contact was transient in nature. Moreover, could activate notably infiltration neutrophils macrophages, proliferation, although dependent did not require constant them. These findings provide fresh perspective cells' role

Language: Английский

Oxidative stress: Roles in skeletal muscle atrophy DOI
Han Zhang,

Guangdong Qi,

Kexin Wang

et al.

Biochemical Pharmacology, Journal Year: 2023, Volume and Issue: 214, P. 115664 - 115664

Published: June 16, 2023

Language: Английский

Citations

104
Rac1: A Regulator of Cell Migration and a Potential Target for Cancer Therapy DOI Creative Commons
Ning Ma,

Erqian Xu,

Qing Luo

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(7), P. 2976 - 2976

Published: March 27, 2023

Cell migration is crucial for physiological and pathological processes such as morphogenesis, wound repair, immune response cancer invasion/metastasis. There are many factors affecting cell migration, the regulatory mechanisms complex. Rac1 a GTP-binding protein with small molecular weight belonging to Rac subfamily of Rho GTPase family. As key molecule in regulating participates signal transduction from external actin cytoskeleton promotes establishment polarity which plays an important role In this review, we firstly introduce structure activity regulation Rac1, then summarize invasion/metastasis other processes. We also discuss highlight it potential target therapy. Finally, current state well future challenges area considered. Understanding mechanism can provide fundamental insights into Rac1-related progression further help us develop novel intervention strategies therapy clinic.

Language: Английский

Citations

53
Three-dimensional imaging studies in mice identify cellular dynamics of skeletal muscle regeneration DOI

Brittany C. Collins,

Jacob B. Shapiro, Mya M. Scheib

et al.

Developmental Cell, Journal Year: 2024, Volume and Issue: 59(11), P. 1457 - 1474.e5

Published: April 2, 2024

Language: Английский

Citations

21
The emergence of the stem cell niche DOI Creative Commons
Michael R. Hicks, April D. Pyle

Trends in Cell Biology, Journal Year: 2022, Volume and Issue: 33(2), P. 112 - 123

Published: Aug. 4, 2022

Niches coevolve with the maturation of progenitor and stem cells from development through to adulthood.Fetal progenitors in emerging niches are highly expansive, whereas adult regulate cell quiescence.Fetal less able occupy during transplantation.Niche formation is stepwise, involving cell–cell contact, followed by cell–extracellular matrix affect polarity maturation.Emerging use similar pathways mechanisms regeneration.Understanding niche may improve quality quantity within niches.Supportive types different homeostatic niches.Human pluripotent lineages can be used model components vitro vivo. Stem composed dynamic microenvironments that support over a lifetime. The distinct because its main role build organ systems development. Emerging mature stages form enable proper support. As niches, this review highlights how differences skeletal muscle microenvironment influence versus satellite (SC) muscle, which among most regenerative tissue systems. We contrast intrinsic properties between throughout adulthood. describe new applications for generating human (hPSCs) using developmental principles highlight potential regeneration therapeutics.

Language: Английский

Citations

60
Biomaterials‐Based Technologies in Skeletal Muscle Tissue Engineering DOI
Wei Luo, Hanli Zhang, Renwen Wan

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(18)

Published: May 7, 2024

For many clinically prevalent severe injuries, the inherent regenerative capacity of skeletal muscle remains inadequate. Skeletal tissue engineering (SMTE) seeks to meet this clinical demand. With continuous progress in biomedicine and related technologies including micro/nanotechnology 3D printing, numerous studies have uncovered various intrinsic mechanisms regulating regeneration developed tailored biomaterial systems based on these understandings. Here, structure process are discussed diverse derived from explored detail. Biomaterials serve not merely as local niches for cell growth, but also scaffolds endowed with structural or physicochemical properties that provide cues such topographical, electrical, mechanical signals. They can act delivery stem cells bioactive molecules been shown key participants endogenous repair cascades. To achieve bench-to-bedside translation, typical effect enabled by potential underlying molecular summarized. Insights into roles biomaterials SMTE cellular perspectives provided. Finally, advancement provided, which gene therapy, exosomes, hybrid may hold promise make important contributions.

Language: Английский

Citations

11
Psat1-generated α-ketoglutarate and glutamine promote muscle stem cell activation and regeneration DOI Open Access

Veronica Ciuffoli,

Xuesong Feng,

Kan Jiang

et al.

Genes & Development, Journal Year: 2024, Volume and Issue: 38(3-4), P. 151 - 167

Published: Feb. 1, 2024

By satisfying bioenergetic demands, generating biomass, and providing metabolites serving as cofactors for chromatin modifiers, metabolism regulates adult stem cell biology. Here, we report that a branch of glycolysis, the serine biosynthesis pathway (SBP), is activated in regenerating muscle cells (MuSCs). Gene inactivation metabolomics revealed Psat1, one three SBP enzymes, controls MuSC activation expansion myogenic progenitors through production metabolite α-ketoglutarate (α-KG) α-KG-generated glutamine. Psat1 ablation resulted defective MuSCs impaired regeneration. α-KG, glutamine were reduced old mice. α-KG or re-established appropriate regeneration conditional −/− mice These findings contribute insights into metabolic role during suggest potential therapeutic interventions to ameliorate aging.

Language: Английский

Citations

10
Extracellular matrix: Brick and mortar in the skeletal muscle stem cell niche DOI Creative Commons
Svenja C. Schüler, Yuguo Liu,

Simon Dumontier

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 10

Published: Nov. 29, 2022

The extracellular matrix (ECM) is an interconnected macromolecular scaffold occupying the space between cells. Amongst other functions, ECM provides structural support to tissues and serves as a microenvironmental niche that conveys regulatory signals Cell-matrix adhesions, which link cytoskeleton, are dynamic multi-protein complexes containing surface receptors intracellular effectors control various downstream pathways. In skeletal muscle, most abundant tissue of body, each individual muscle fiber its associated stem cells (MuSCs) surrounded by layer referred basal lamina. core lamina consists self-assembling polymeric laminins network collagens tether proteoglycans, provide lateral crosslinking, establish collateral associations with cell receptors, serve sink reservoir for growth factors. Skeletal also contains fibrillar collagenous interstitial plays important role in determining elasticity, connects laminae other, secreting mesenchymal fibroblast-like types blood vessels. During regeneration populations expand contribute transitional fibronectin-rich regenerative instructs angiogenesis MuSC function. Here, we comprehensive overview health disease outline orchestrating

Language: Английский

Citations

36
ATF3 induction prevents precocious activation of skeletal muscle stem cell by regulating H2B expression DOI Creative Commons
Suyang Zhang,

Feng Yang,

Yile Huang

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Aug. 17, 2023

Skeletal muscle stem cells (also called satellite cells, SCs) are important for maintaining tissue homeostasis and damage-induced regeneration. However, it remains poorly understood how SCs enter cell cycle to become activated upon injury. Here we report that AP-1 family member ATF3 (Activating Transcription Factor 3) prevents SC premature activation. Atf3 is rapidly transiently induced in Short-term deletion of accelerates acute injury-induced regeneration, however, its long-term exhausts the pool thus impairs The loss also provokes activation during voluntary exercise enhances endurance exercise. Mechanistically, directly activates transcription Histone 2B genes, whose reduction nucleosome displacement gene required Finally, ATF3-dependent H2B expression genome instability replicative senescence SCs. Therefore, this study has revealed a previously unknown mechanism preserving population by actively suppressing precocious activation, which key regulator.

Language: Английский

Citations

22
Postnatal skeletal muscle myogenesis governed by signal transduction networks: MAPKs and PI3K–Akt control multiple steps DOI
T. Endo

Biochemical and Biophysical Research Communications, Journal Year: 2023, Volume and Issue: 682, P. 223 - 243

Published: Sept. 27, 2023

Language: Английский

Citations

14
Granular Hydrogels Improve Myogenic Invasion and Repair after Volumetric Muscle Loss DOI Creative Commons
Gabrielle I. Tanner,

Leia Schiltz,

Niharika Narra

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 8, 2024

Abstract Skeletal muscle injuries including volumetric loss (VML) lead to excessive tissue scarring and permanent functional disability. Despite its high prevalence, there is currently no effective treatment for VML. Bioengineering interventions such as biomaterials that fill the VML defect support cell growth are a promising therapeutic strategy. However, traditional developed this purpose lack pore features needed infiltration. The present study investigates first time, impact of granular hydrogels on repair – hypothesizing their flowability will permit conformable filling site inherent porosity invasion native myogenic cells, leading repair. Small large microparticle fragments prepared from photocurable hyaluronic acid polymer via extrusion fragmentation facile size sorting. In assembled hydrogels, particle degree packing significantly influence features, rheological behavior, injectability. Using mouse model VML, it demonstrated that, in contrast bulk early‐stage (satellite invasion) late‐stage (myofiber regeneration) processes. Together, these results highlight potential injectable porous supporting endogenous after severe injury.

Language: Английский

Citations

6