Spatially defined microenvironment for engineering organoids DOI
Yilan Zhang,

Fukang Qi,

Peng Chen

et al.

Biophysics Reviews, Journal Year: 2024, Volume and Issue: 5(4)

Published: Oct. 18, 2024

In the intricately defined spatial microenvironment, a single fertilized egg remarkably develops into conserved and well-organized multicellular organism. This observation leads us to hypothesize that stem cells or other seed cell types have potential construct fully structured functional tissues organs, provided cues are appropriately configured. Current organoid technology, however, largely depends on spontaneous growth self-organization, lacking systematic guided intervention. As result, structures replicated in vitro often emerge disordered sparse manner during phases. Although existing organoids made significant contributions many aspects, such as advancing our understanding of development pathogenesis, aiding personalized drug selection, well expediting development, their creating large-scale implantable tissue organ constructs, constructing multicomponent microphysiological systems, together with functioning at metabolic levels remains underutilized. Recent discoveries demonstrated definition factors not only induces directional migration but also formation assembloids multiple regional identities. opens new avenues for innovative engineering higher-order organoids. Concurrently, organization microenvironmental cues, physical stresses, mechanical loads, material composition, has been minimally explored. review delves burgeoning field focus control. It offers insight molecular principles, expected outcomes, applications, envisioning future perspective this domain.

Language: Английский

Spatially defined microenvironment for engineering organoids DOI
Yilan Zhang,

Fukang Qi,

Peng Chen

et al.

Biophysics Reviews, Journal Year: 2024, Volume and Issue: 5(4)

Published: Oct. 18, 2024

In the intricately defined spatial microenvironment, a single fertilized egg remarkably develops into conserved and well-organized multicellular organism. This observation leads us to hypothesize that stem cells or other seed cell types have potential construct fully structured functional tissues organs, provided cues are appropriately configured. Current organoid technology, however, largely depends on spontaneous growth self-organization, lacking systematic guided intervention. As result, structures replicated in vitro often emerge disordered sparse manner during phases. Although existing organoids made significant contributions many aspects, such as advancing our understanding of development pathogenesis, aiding personalized drug selection, well expediting development, their creating large-scale implantable tissue organ constructs, constructing multicomponent microphysiological systems, together with functioning at metabolic levels remains underutilized. Recent discoveries demonstrated definition factors not only induces directional migration but also formation assembloids multiple regional identities. opens new avenues for innovative engineering higher-order organoids. Concurrently, organization microenvironmental cues, physical stresses, mechanical loads, material composition, has been minimally explored. review delves burgeoning field focus control. It offers insight molecular principles, expected outcomes, applications, envisioning future perspective this domain.

Language: Английский

Citations

0