bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 17, 2024
Understanding
the
mechanisms
of
hypoblast
development
and
its
role
in
implantation
is
critical
for
improving
farm
animal
reproduction,
but
it
hampered
by
lack
research
models.
Here
we
report
that
a
chemical
cocktail
(FGF4,
BMP4,
IL-6,
XAV939,
A83-01)
enables
de
novo
derivation
long-term
culture
bovine
extraembryonic
endoderm
cells
(bXENs).
Transcriptomic
epigenomic
analyses
confirmed
identity
bXENs
revealed
they
are
resemble
lineages
early
peri-implantation
embryos.
We
showed
help
maintain
stemness
ESCs
prevent
them
from
differentiation.
In
presence
signaling
sustaining
bXENs,
growth
progression
epiblasts
also
facilitated
developing
pre-implantation
embryo.
Furthermore,
through
3D
assembly
with
TSCs,
developed
an
improved
blastocyst
like
structure
(bovine
blastoid)
resembles
blastocyst.
The
XENs
blastoids
established
this
study
represent
accessible
vitro
models
understanding
reproductive
efficiency
livestock
species.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 8, 2024
Abstract
Oct4
is
a
commonly
known
marker
of
pluripotent
stem
cells
as
well
one
the
key
factors
required
for
pluripotency
induction.
Its
gene
(
Pou5f1
)
subject
to
complicated
regulation
through
distal
and
proximal
enhancers.
Noteworthy,
this
protein
also
plays
an
important
role
in
primitive
endoderm
(PrE)
specification,
though
mechanisms
driving
its
expression
during
process
are
still
unknown.
Here
we
show
that
KH-domain
Pcbp1
occupies
poly(C)-sites
enhancers,
but
knockout
does
not
affect
level
ESCs.
On
contrary,
essential
timely
downregulation
upon
differentiation
signals.
Residual
turn
leads
PrE
phenotype
reminiscent
ESCs
constitutively
expressing
Oct4.
Overall,
our
results
point
transcriptional
regulator
Pou5f1,
purported
synchronize
decline
with
network
shutdown
differentiation.
being
outside
loss
functions
factor
acts
specifier.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 12, 2024
Abstract
Embryonic
stem
cell
research
has
uncovered
different
requirements
for
WNT/β-catenin
signalling
in
human
naïve
pluripotent
cells
compared
to
the
mouse
paradigm.
It
is
therefore
important
study
directly
models
of
early
development.
Since
TCF/LEF
factors
mediate
regulation
target
genes
downstream
signalling,
we
studied
expression
and
protein
localisation
four
by
analysing
vitro
“snapshots”
development,
leveraging
primed
as
well
extraembryonic
embryonic
lineages.
Strikingly,
comprehensively
confirm
clear
differences
between
cells,
suggesting
species-specific
WNT
that
may
reflect
states
pluripotency.
Human
ES
express
very
low
TCF7L1,
unlike
their
counterparts.
TCF7L2
robustly
expressed
ES-derived
trophectoderm
cells.
In
activation
WNT/β-Catenin
required
induce
both
TCF7
LEF1
,
concomitant
with
hallmark
gastrulation
markers.
This
benchmarks
differential
throughout
embryo
development
requires
further
investigation.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(23), P. 12862 - 12862
Published: Nov. 29, 2024
Advances
in
three-dimensional
culture
technologies
have
facilitated
the
development
of
synthetic
embryo
models,
such
as
blastoids,
through
co-culturing
diverse
stem
cell
types.
These
vitro
models
enable
precise
investigation
developmental
processes,
including
gastrulation,
neurulation,
and
lineage
specification,
thereby
advancing
our
understanding
early
embryogenesis.
By
providing
controllable,
ethically
viable
platforms,
they
help
circumvent
limitations
vivo
mammalian
studies
contribute
to
developing
regenerative
medicine
strategies.
Nonetheless,
ethical
challenges,
particularly
regarding
human
applications,
persist.
Comparative
across
various
species—such
mice,
humans,
non-human
primates,
ungulates,
like
pigs
cattle—offer
crucial
insights
into
both
species-specific
conserved
mechanisms.
In
this
review,
we
outline
differences
embryonic
discuss
recent
advancements
models.
Specifically,
focus
on
latest
research
involving
cattle,
provide
a
comprehensive
overview
improvements
technology.
biology,
improve
model
efficiency,
guide
new
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 17, 2024
Understanding
the
mechanisms
of
hypoblast
development
and
its
role
in
implantation
is
critical
for
improving
farm
animal
reproduction,
but
it
hampered
by
lack
research
models.
Here
we
report
that
a
chemical
cocktail
(FGF4,
BMP4,
IL-6,
XAV939,
A83-01)
enables
de
novo
derivation
long-term
culture
bovine
extraembryonic
endoderm
cells
(bXENs).
Transcriptomic
epigenomic
analyses
confirmed
identity
bXENs
revealed
they
are
resemble
lineages
early
peri-implantation
embryos.
We
showed
help
maintain
stemness
ESCs
prevent
them
from
differentiation.
In
presence
signaling
sustaining
bXENs,
growth
progression
epiblasts
also
facilitated
developing
pre-implantation
embryo.
Furthermore,
through
3D
assembly
with
TSCs,
developed
an
improved
blastocyst
like
structure
(bovine
blastoid)
resembles
blastocyst.
The
XENs
blastoids
established
this
study
represent
accessible
vitro
models
understanding
reproductive
efficiency
livestock
species.
