Generation of High-fidelity Blastocyst-like Structures from Porcine Expanded Pluripotent stem cell via Chemically inducing cell plasticity DOI Open Access

Zheng Liao,

Xin Qi,

Shijie Yuan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 24, 2024

Summary Understanding the mechanisms of blastocyst formation and implantation is crucial for advancing farm animal reproduction. However, research in this area often hindered by limited availability embryos. In study, we developed an efficient method to generate blastocyst-like structures (termed blastoids) from porcine expanded pluripotent stem cells (pEPSCs) through chemical induction with efficiency up 80%. These blastoids closely resemble natural blastocysts terms morphology, cell composition, single-cell transcriptomes. The tissue over time culture, resembling morphology gastrulation-stage This innovative approach not only provides a robust vitro model studying early embryogenesis pigs but also holds potential improving reproductive understanding developmental processes large species. development represents significant advancement regenerative medicine biology.

Language: Английский

Generation of High-fidelity Blastocyst-like Structures from Porcine Expanded Pluripotent stem cell via Chemically inducing cell plasticity DOI Open Access

Zheng Liao,

Xin Qi,

Shijie Yuan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 24, 2024

Summary Understanding the mechanisms of blastocyst formation and implantation is crucial for advancing farm animal reproduction. However, research in this area often hindered by limited availability embryos. In study, we developed an efficient method to generate blastocyst-like structures (termed blastoids) from porcine expanded pluripotent stem cells (pEPSCs) through chemical induction with efficiency up 80%. These blastoids closely resemble natural blastocysts terms morphology, cell composition, single-cell transcriptomes. The tissue over time culture, resembling morphology gastrulation-stage This innovative approach not only provides a robust vitro model studying early embryogenesis pigs but also holds potential improving reproductive understanding developmental processes large species. development represents significant advancement regenerative medicine biology.

Language: Английский

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