Seminars in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 171, P. 103611 - 103611
Published: May 2, 2025
Language: Английский
Cell Regeneration, Journal Year: 2025, Volume and Issue: 14(1)
Published: March 23, 2025
Abstract The formation of a blood vessel network is crucial for organ development and regeneration. Over the past three decades, central molecular mechanisms governing growth have been extensively studied. Recent evidence indicates that vascular endothelial cells—the specialized cells lining inner surface vessels—exhibit significant heterogeneity to meet specific needs different organs. This review focuses on current understanding cell heterogeneity, which includes both intra-organ inter-organ heterogeneity. Intra-organ encompasses arterio-venous tip-stalk specialization, while refers organ-specific transcriptomic profiles functions. Advances in single-cell RNA sequencing (scRNA-seq) enabled identification new subpopulations comparison gene expression patterns across subsets cells. Integrating scRNA-seq with other high-throughput technologies promises deepen our at epigenetic level spatially resolved context. To further explore human organoids offer powerful tools studying function three-dimensional culture systems investigating endothelial-tissue interactions using Developing presents unique opportunities unravel its implications disease. Emerging technologies, such as organoids, are poised transform pave way innovative therapeutic strategies address diseases. Graphical
Language: Английский
Citations
1
Cell stem cell, Journal Year: 2025, Volume and Issue: 32(2), P. 177 - 178
Published: Feb. 1, 2025
Language: Английский
Citations
0
Acta Physiologica, Journal Year: 2025, Volume and Issue: 241(5)
Published: March 29, 2025
The microvascular system is essential for delivering oxygen and nutrients to tissues while removing metabolic waste. During pregnancy, the uteroplacental undergoes extensive remodeling meet increased demands of fetus. Key adaptations include vessel dilation increases in vascular volume, density, permeability, all which ensure adequate placental perfusion maintaining stable maternal blood pressure. Structural functional abnormalities microvasculature are associated with various gestational complications, posing both immediate long-term risks health mother infant. In this review, we describe changes microvessels during discuss pathogenic mechanisms underlying diseases such as preeclampsia, fetal growth restriction, diabetes, summarize current clinical research approaches monitoring health. We also provide an update on models complications explore solutions several unresolved challenges. With advancements techniques, anticipate significant progress understanding managing these diseases, ultimately leading new therapeutic strategies improve
Language: Английский
Citations
0
Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 12
Published: April 17, 2025
Venous/lymphatic malformations (VM/LM) represent a group of vascular anomalies that share similarities in diagnosis, classification, management, and pathogenesis. Despite growing research interest, no comprehensive bibliometric analysis has been conducted to map the knowledge landscape evolution VM/LM studies. We searched Web Science Core Collection database for VM/LM-related publications from January 2003 December 2023. multiple analytical tools including VOSviewer, CiteSpace, R package 'bibliometrix' were used analyze publication trends, collaboration networks, hotspots. 4,747 with 57,893 references enrolled analyzed. The number is generally erupted 2019. USA, China, Japan are top 3 countries publication. main study centers scattered Europe, Japan. Fishman SJ published most. Vikkula M was cited Journal Pediatric Surgery basis contains wide range concepts classification/differential genetic causes, Sirolimus treatment, localized intravascular coagulopathy, percutaneous treatment. topic trend mainly changed after 2019 targeted therapy. This first demonstrating field's rapid growth shifting Four key future directions emerged: molecular-level diagnosis precision therapy, advanced sclerotherapy techniques, artificial intelligence applications. International between needs be strengthen. These findings provide an objective foundation investigations.
Language: Английский
Citations
0
Biotechnology and Bioengineering, Journal Year: 2025, Volume and Issue: unknown
Published: March 25, 2025
Differentiating endothelial cells (ECs) from human pluripotent stem (hPSCs) typically takes 2 weeks and requires parameter optimization. Overexpression of cell type-specific transcription factors in hPSCs has shown efficient differentiation into various types. ETV2, a crucial factor for fate, can be overexpressed to induce rapid facile EC (iETV2-ECs). We developed two-stage strategy which involves differentiating inducible ETV2-overexpressing basal induction medium during stage I expanding them an II. By optimizing seeding density composition, we achieved 99% pure CD31+ CD144+ iETV2-ECs without sorting 5 days. demonstrated vitro angiogenesis potential, LDL uptake, cytokine response. Transcriptomic comparisons revealed similar gene expression profiles between traditionally differentiated ECs. Additionally, responded Wnt signaling agonist TGFβ inhibitor acquire brain phenotypes, making scalable source applications including blood-brain barrier modeling.
Language: Английский
Citations
0
Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown
Published: April 26, 2025
Abstract Organoids provide 3D structures that replicate native tissues in biomedical research. The development of vascular networks within organoids enables oxygen and nutrient delivery while facilitating metabolic waste removal, which supports organoid growth maturation. Recent studies demonstrate vascularized models offer insights into tissue interactions promote regeneration. However, the current limitations establishing functional affect growth, viability, clinical translation potential. This review examines organoids, including mechanisms angiogenesis vasculogenesis, construction strategies, applications. approaches are categorized vivo vitro methods, with analysis their specific advantages limitations. also discusses emerging techniques such as bioprinting gene editing for improving vascularization integration organoid‐based therapies. Current developments indicate potential applications modeling human diseases developing therapeutic contributing to advances translational
Language: Английский
Citations
0
Seminars in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 171, P. 103611 - 103611
Published: May 2, 2025
Language: Английский
Citations
0