Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

Stromal progenitor cells of bone marrow origin are non-hematopoietic that give rise to osteoblasts and adipocytes in the postnatal organism. Marrow stromal (MSCs) (also known as skeletal stem cells)are currently being employed a large number clinical trials for regenerative purposes post in vitro expansion. However, outcome has been variable, which might part be due heterogeneity lack defined cell product with molecular signature favors tissue regeneration. In this study, we determined cellular primary cultures examined how inter-donor variation subpopulation composition contributes differentiation potential cultures. We profiled 136,014 progenitors from 26 donors identified 5 subpopulations were linked distinct bone-related pathways genetic traits mineral density morphology. Abundancy one cluster characterized by high expression ITGA11 genes related matrix function, collagen organization, elevated upon lineage commitment was positively correlated osteoblastic capacity vitro. addition, ITGA11 protein predictive marker mineralization ectopic formation vivo. Sorting into ITGA11^high ITGA11^low established low potential, respectively. Our findings corroborate presence extensive among cultured human cells, strongly differs donor donor, can isolating bone-forming feature likely benefit

Language: Английский