Super RNA Pol II domains enhance minor ZGA through 3D interaction to ensure the integrity of major transcriptional waves in late-ZGA mammals DOI Creative Commons
Jingcheng Zhang,

Hengkuan Li,

Linmi Li

et al.

Cell Genomics, Journal Year: 2025, Volume and Issue: unknown, P. 100856 - 100856

Published: May 1, 2025

Zygotic genome activation (ZGA) occurs at distinct stages across mammals, with mice initiating ZGA the 2-cell stage and bovines humans activating process in 4- to 8-cell stages. RNA polymerase II (RNA Pol II) gradually initiates mice, but regulation late-ZGA species remains unclear. Here, profiling bovine embryos identified strong intergenic clusters that boost minor gene expression via chromatin interactions are named super domains (SPDs). CRISPRi perturbation of SPDs decreases genes, whereas knockdown these genes disrupts major embryogenesis. Rapid enhancement also human embryos. Alternatively, mouse porcine oocytes precociously express without SPDs. Thus, appear be an adaptation embryos, promoting comparable levels as early-ZGA species, illuminating species-specific timing.

Language: Английский

Profiling and functional characterization of maternal mRNA translation during mouse maternal-to-zygotic transition DOI Creative Commons
Chunxia Zhang, Meng Wang, Yisi Li

et al.

Science Advances, Journal Year: 2022, Volume and Issue: 8(5)

Published: Feb. 2, 2022

Translational regulation plays an important role in gene expression and function. Although the transcriptional dynamics of mouse preimplantation embryos have been well characterized, global mRNA translation landscape master regulators zygotic genome activation (ZGA) remain unknown. Here, by developing applying a low-input ribosome profiling (LiRibo-seq) technique, we profiled revealed translational during development. We identified marked transition from MII oocytes to zygotes demonstrated that active maternal mRNAs is essential for maternal-to-zygotic (MZT). further showed two factors, Smarcd2 Cyclin T2, whose activated zygotes, are required chromatin reprogramming ZGA, respectively. Our study thus not only filled knowledge gap on mammalian development but also insights into critical function MZT.

Language: Английский

Citations

90
scm6A-seq reveals single-cell landscapes of the dynamic m6A during oocyte maturation and early embryonic development DOI Creative Commons
Huan Yao, Chun‐Chun Gao,

Danru Zhang

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Jan. 19, 2023

N6-methyladenosine (m6A) has been demonstrated to regulate RNA metabolism and various biological processes, including gametogenesis embryogenesis. However, the landscape function of m6A at single cell resolution have not extensively studied in mammalian oocytes or during pre-implantation. In this study, we developed a single-cell sequencing (scm6A-seq) method simultaneously profile methylome transcriptome oocytes/blastomeres cleavage-stage embryos. We found that deficiency leads aberrant clearance consequent low quality Mettl3Gdf9 conditional knockout (cKO) oocytes. further revealed regulates translation stability modified RNAs metaphase II (MII) oocyte-to-embryo transition, respectively. Moreover, observed m6A-dependent asymmetries epi-transcriptome between blastomeres two-cell embryo. scm6A-seq thus allows in-depth investigation into characteristics functions, findings provide invaluable resources for delineating underlying mechanism early embryonic development.

Language: Английский

Citations

61
Dynamic chromatin regulatory programs during embryogenesis of hexaploid wheat DOI Creative Commons
Long Zhao, Yiman Yang, Jinchao Chen

et al.

Genome biology, Journal Year: 2023, Volume and Issue: 24(1)

Published: Jan. 13, 2023

Abstract Background Plant and animal embryogenesis have conserved distinct features. Cell fate transitions occur during in both plants animals. The epigenomic processes regulating plant remain largely elusive. Results Here, we elucidate chromatin transcriptomic dynamics of the most cultivated crop, hexaploid wheat. Time-series analysis reveals stage-specific proximal–distal accessibility concordant with transcriptome changes. Following fertilization, remodeling kinetics H3K4me3, H3K27ac, H3K27me3 differ from that mammals, highlighting considerable species-specific zygotic genome activation. Polycomb repressive complex 2 (PRC2)-mediated deposition is important for embryo establishment. Later H3K27me3, undergo dramatic to establish a permissive environment facilitating access transcription factors cis -elements patterning. Embryonic maturation characterized by increasing decreasing accessibility, which likely participates restricting totipotency while preventing extensive organogenesis. Finally, signatures are correlated biased expression among homeolog triads divergent after polyploidization, revealing an contributor subgenome diversification allohexaploid genome. Conclusions Collectively, present invaluable resource comparative mechanistic regulation crop embryogenesis.

Language: Английский

Citations

50
Epigenetic regulation of early human embryo development DOI Creative Commons
Amy L. Wilkinson, Irene Zorzan, Peter J. Rugg‐Gunn

et al.

Cell stem cell, Journal Year: 2023, Volume and Issue: 30(12), P. 1569 - 1584

Published: Oct. 18, 2023

Studies of mammalian development have advanced our understanding the genetic, epigenetic, and cellular processes that orchestrate embryogenesis uncovered new insights into unique aspects human embryogenesis. Recent studies now produced first epigenetic maps early embryogenesis, stimulating ideas about reprogramming, cell fate control, potential mechanisms underpinning developmental plasticity in embryos. In this review, we discuss these regulation importance for safeguarding development. We also highlight unanswered questions key challenges remain to be addressed.

Language: Английский

Citations

45
Capturing totipotency in human cells through spliceosomal repression DOI
Shiyu Li, Min Yang, Hui Shen

et al.

Cell, Journal Year: 2024, Volume and Issue: 187(13), P. 3284 - 3302.e23

Published: June 1, 2024

Language: Английский

Citations

23
An atlas of transcription initiation reveals regulatory principles of gene and transposable element expression in early mammalian development DOI Creative Commons
Marlies E. Oomen, Diego Rodriguez‐Terrones, Mayuko Kurome

et al.

Cell, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

5
Metabolic remodelling during early mouse embryo development DOI
Jing Zhao, Ke Yao, Hua Yu

et al.

Nature Metabolism, Journal Year: 2021, Volume and Issue: 3(10), P. 1372 - 1384

Published: Oct. 14, 2021

Language: Английский

Citations

88
Evolutionary epigenomic analyses in mammalian early embryos reveal species-specific innovations and conserved principles of imprinting DOI Creative Commons
Xukun Lu, Yu Zhang, Lijuan Wang

et al.

Science Advances, Journal Year: 2021, Volume and Issue: 7(48)

Published: Nov. 24, 2021

Evolutionary analyses reveal conservation and divergence of epigenetic transition during early development across mammals.

Language: Английский

Citations

62
Epigenetic Reprogramming in Early Animal Development DOI

Zhenhai Du,

Ke Zhang, Wei Xie

et al.

Cold Spring Harbor Perspectives in Biology, Journal Year: 2021, Volume and Issue: 14(6), P. a039677 - a039677

Published: Aug. 16, 2021

Dramatic nuclear reorganization occurs during early development to convert terminally differentiated gametes a totipotent zygote, which then gives rise an embryo. Aberrant epigenome resetting severely impairs embryo and even leads lethality. How the epigenomes are inherited, reprogrammed, reestablished in this critical developmental period has gradually been unveiled through rapid of technologies including ultrasensitive chromatin analysis methods. In review, we summarize latest findings on epigenetic reprogramming gametogenesis embryogenesis, how it contributes gamete maturation parental-to-zygotic transition. Finally, highlight key questions that remain be answered fully understand regulation development.

Language: Английский

Citations

57
Epigenetics of pregnancy: looking beyond the DNA code DOI Creative Commons
Daniela Zuccarello, Ugo Sorrentino,

Valeria Brasson

et al.

Journal of Assisted Reproduction and Genetics, Journal Year: 2022, Volume and Issue: 39(4), P. 801 - 816

Published: March 17, 2022

Abstract Epigenetics is the branch of genetics that studies different mechanisms influence gene expression without direct modification DNA sequence. An ever-increasing amount evidence suggests such regulatory processes may play a pivotal role both in initiation pregnancy and later embryonic fetal development, thus determining long-term effects even adult life. In this narrative review, we summarize current knowledge on epigenetics pregnancy, from its most studied well-known to new frontiers epigenetic regulation, as ncRNAs gestational environment brain development. Epigenetic are dynamic phenomenon responds maternal–fetal environmental factors, which can modify embryo-fetal development during various phases. Therefore, also recapitulate notable factors affect prenatal maternal nutrition, stress hormones, microbiome, teratogens, focusing their ability cause modifications ultimately fetus. Despite promising advancements more experience data topic still needed. A better understanding regulation could fact prove valuable towards management physiological pregnancies assisted reproduction treatments, other than allowing comprehend origin multifactorial pathological conditions neurodevelopmental disorders.

Language: Английский

Citations

45