Environmental
soils
are
natural
reservoirs
of
unexplored
microbes,
including
potentially
beneficial
microbes
to
improve
plant
performance.
Here,
we
isolated
75
bacterial
strains
from
surface-sterilized
roots
_Arabidopsis
thaliana_
(Arabidopsis)
grown
in
a
soil
derived
an
alder
swamp.
Culture-dependent
isolation
individual
the
followed
by
monoassociation-based
screening
identified
seven
bacteria
that
promoted
Arabidopsis
seedling
weight.
Of
those,
new
species
_Dyella_
genus
which
increased
biomass
tomato
and
seedlings
agar
as
well
shoot
both
swamp
potting
soil.
_Dyella
sp_.
A4
specifically
elongation
lateral
without
affecting
root
number
primary
elongation.
The
expands
toolbox
biostimulants
for
growth
promotion
via
modulating
architecture.
Frontiers in Sustainable Food Systems,
Journal Year:
2025,
Volume and Issue:
9
Published: Feb. 12, 2025
Introduction
The
effect
of
co-inoculation
with
plant
growth-promoting
bacteria
on
the
microbiome
soybean
roots
was
investigated
in
a
field
experiment.
Soybean
plants
were
inoculated
Bacillus
subtilis
,
aryabhattai
Streptomyces
sp.,
and
Saccharopolyspora
spinosa
compared
to
control
treatment
that
received
mineral
fertilization.
Methods
yield
parameters
endophytic
evaluated.
Results
No
significant
differences
observed
among
treatments,
suggesting
microbial
inoculation
can
serve
as
an
alternative
fertilization
without
compromising
productivity.
Among
most
abundant
genera,
there
high
prevalence
members
phylum
Proteobacteria
(21
top
25
genera).
Overall,
genera
these
phyla
represented
88.61%
samples
average.
There
also
Bacteroidetes
(2/25),
Actinobacteria
(1/25),
Firmicutes
(1/25).
massive
presence
Bradyrhizobium
which
71.22%
sequences
at
genus
level,
remarkable.
all
samples,
except
for
(ST
treatment),
whose
abundance
only
12.66%.
Co-occurrence
network
analysis
revealed
changes
community
structure
considered
hubs.
Discussion
These
findings
demonstrate
potential
modulate
root
enhance
colonization
B.
japonicum
may
contribute
improving
efficiency
this
symbiont
promoting
growth.
Further
research
is
required
elucidate
mechanisms
underlying
interactions
their
implications
Environmental
soils
are
natural
reservoirs
of
unexplored
microbes,
including
potentially
beneficial
microbes
to
improve
plant
performance.
Here,
we
isolated
75
bacterial
strains
from
surface-sterilized
roots
_Arabidopsis
thaliana_
(Arabidopsis)
grown
in
a
soil
derived
an
alder
swamp.
Culture-dependent
isolation
individual
the
followed
by
monoassociation-based
screening
identified
seven
bacteria
that
promoted
Arabidopsis
seedling
weight.
Of
those,
new
species
_Dyella_
genus
which
increased
biomass
tomato
and
seedlings
agar
as
well
shoot
both
swamp
potting
soil.
_Dyella
sp_.
A4
specifically
elongation
lateral
without
affecting
root
number
primary
elongation.
The
expands
toolbox
biostimulants
for
growth
promotion
via
modulating
architecture.
