Impact of Water Stress on Metabolic Intermediates and Regulators in Broccoli Sprouts, and Cellular Defense Potential of Their Extracts

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 632 - 632

Published: Jan. 13, 2025

Drought and flood (water stress) alter plant metabolism, impacting the phytochemical content biological effects. Using spectrophotometric, HPLC, electrophoretic methods, we analyze effects of water stress on broccoli (Brassica oleracea L. convar. botrytis (L.) Alef. var. cymosa Duch.) sprouts. differently influenced chlorophylls, carotenoids, porphyrins, with drought having a stronger inhibitory effect chlorophyll a, total chlorophyll, porphyrins. Carotenoids glucosinolates increased under but decreased flooding, suggesting that these compounds play crucial role in tolerance. Nitrate from 13.11 ± 1.05 mg/g dw to 22.41 1.20 flooding 5.17 1.03 dw, oxalic acid was reduced by only (from 48.94 1.30 46.43 0.64 dw). Flood proteins 29%, phenolics 15%, flavonoids 10%, flavonols 11%, tannins 36%, proanthocyanidins 19%, while 23%. Total were 29% 7%, respectively, hydroxycinnamic acids 13%. Both types individual polyphenols differently: diminished ferulic 17% sinapic 30%, reversed enhanced kaempferol 22%. These compounds, along proline (which 139% drought), emerged as biomarkers stress. impacted antioxidant capacity more significantly, drought-stressed extracts better protected plasmid DNA against oxidative damage. findings underline metabolic plasticity sprouts their potential targeted crop management for resilience.

Language: Английский

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Combined Bulked Segregant Analysis-Sequencing and Transcriptome Analysis to Identify Candidate Genes Associated with Cold Stress in Brassica napus L

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1148 - 1148

Published: Jan. 28, 2025

Cold tolerance in rapeseed is closely related to its growth, yield, and geographical distribution. However, the mechanisms underlying cold resistance remain unclear. This study aimed explore genes provide new insights into molecular of rapeseed. Rapeseed M98 (cold-sensitive line) D1 (cold-tolerant were used as parental lines. In their F2 population, 30 seedlings with lowest damage levels highest selected construct cold-tolerant cold-sensitive pools, respectively. The two pools lines analyzed using bulk segregant sequencing (BSA-seq). G’-value analysis indicated a single peak on Chromosome C09 candidate interval, which had 2.59 Mb segment 69 genes. Combined time-course weighted gene co-expression network analyses performed at seven time points reveal genetic basis two-parent response low temperatures. Twelve differentially expressed primarily involved plant identified. BSA-seq transcriptome revealed BnaC09G0354200ZS, BnaC09G0353200ZS, BnaC09G0356600ZS Quantitative real-time PCR validation was consistent RNA-seq. facilitates exploration

Language: Английский

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The Aldehyde Dehydrogenase Superfamily in Brassica napus L.: Genome-Wide Identification and Expression Analysis Under Low-Temperature Conditions

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2373 - 2373

Published: March 6, 2025

The Aldehyde Dehydrogenase (ALDH) superfamily comprises a group of NAD+ or NADP+-dependent enzymes that play essential roles in responding to abiotic stresses plants. In Brassica napus L., however, the increasing frequency extremely low temperatures during winter recent years has significantly affected both yield and quality. This study conducted genome-wide screening ALDH genes, analyzing their gene structures, evolutionary relationships, protein physicochemical properties, expression patterns under low-temperature stress explore function cold tolerance L. A total six BnALDH genes with significant differences levels were verified utilizing quantitative real-time polymerase chain reaction (qRT-PCR), revealing BnALDH11A2, BnALDH7B2, BnALDH3F5, BnALDH12A3, BnALDH2B6, BnALDH7B3 all exhibited higher cold-tolerant material 24W233 compared cold-sensitive 24W259. Additionally, single nucleotide polymorphism (SNP) BnALDH11A2 promoter region shows between (24W233) (24W259) varieties, it may be associated these two varieties. comprehensive analysis offers valuable insights into role family adaptation genetic resources for development novel cultivars.

Language: Английский

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Transcriptome and Physio-Biochemical Profiling Reveals Differentially Expressed Genes in Seedlings from Aerial and Subterranean Seeds Subjected to Drought Stress in Amphicarpaea edgeworthii Benth

Agronomy, Journal Year: 2025, Volume and Issue: 15(3), P. 735 - 735

Published: March 19, 2025

Drought stress represents a prevalent environmental challenge that significantly impedes plant growth. The Chinese hog-peanut (Amphicarpaea edgeworthii Benth.), an amphicarpic legume, can produce both aerial seeds (ASs) and subterranean (SSs). However, it is largely unknown whether there are differences between the seedlings from ASs SSs in response to drought stress. In this study, 30-day old AS SS of A. subjected by withholding watering for five or ten days. Then, we identify morphological physio-biochemical characteristics under Following days treatment, contents proline (PRO) malondialdehyde (MDA), root shoot ratio, rate water loss were increased, whereas chlorophyll content relative decreased seedlings. Moreover, compared seedlings, accumulated more hydrogen peroxide (H2O2) while exhibiting lower peroxidase (POD) superoxide dismutase (SOD) activities after exposure These findings indicate susceptible To drought-associated genes reveal mechanisms underlying adaptability performed RNA-seq-based transcriptomic analysis exposed We identified 1317 2029 differentially expressed (DEGs) post-drought respectively, 1793 DEGs treatment normal (CK). commonly enriched response-related GO terms. Furthermore, hundreds transcription factor (TF) among treatment. Notably, ERF, bHLH, NAC, C2H2 families predominant following MYB-related, WRKY suggest TFs may play crucial roles

Language: Английский

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