Histological and transcriptomic insights into the interaction between grapevine and Colletotrichum viniferum DOI Creative Commons

Mengru Dou,

Yuhang Li, Yu Hao

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 16, 2024

Introduction Grape is of high economic value. Colletotrichum viniferum , a pathogen causing grape ripe rot and leaf spot, threatens production quality. Methods This study investigates the interplay between C. by Cytological transcriptome sequencing. Results Different grapevine germplasms, V. vinifera cv. Thompson Seedless (TS), labrusca accession Beaumont (B) piasezkii Liuba-8 (LB-8) were classified as highly sensitive, moderate resistant to respectively. analysis reveals distinct differences susceptible grapes post-inoculation, including faster development, longer germination tubes, normal appressoria absence white secretions in host grapevine. To understand pathogenic mechanisms sequencing was performed on “TS” identifying 236 differentially expressed genes. These included 56 effectors, 36 carbohydrate genes, 5 P450 10 genes involved secondary metabolism. Fungal effectors are known pivotal factors that modulate plant immunity affect disease development. Agrobacterium -mediated transient transformation Nicotiana benthamiana screened (CvA13877, CvA01508, CvA05621, CvA00229, CvA07043, CvA05569, CvA12648, CvA02698, CvA14071 CvA10999) inhibited INF1 (infestans 1, P. infestans PAMP elicitor) induced cell death 2 (CvA02641 CvA11478) death. Additionally, response identified related hormone signaling ( TGA PR1 ETR ERF1/2 ), resveratrol biosynthesis STS phenylpropanoid PAL COMT photosynthetic antenna proteins Lhca Lhcb transcription WRKY NAC MYB ERF GATA bHLH SBP ROS (reactive oxygen species) clearance CAT GSH POD SOD disease-related LRR RPS2 GST ). Discussion highlights potential functional diversity effectors. Our findings lay foundation for further research infection identification targets grape.

Language: Английский

Construction of a Microenvironment‐Responsive Antibacterial Hydrogel Packed With Immunomodulatory Carbonized Polymer Dots for Periodontitis Treatment From Multiple Perspectives DOI
Nuo Li, Xiangyu Yan, Mingyue Cheng

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Abstract Periodontitis is an increasingly prevalent oral inflammatory disease, whose treatment faces potent challenges because of recurrent bacterial infections, excessive reactive oxygen species (ROS) production, as well unrelenting inflammation‐induced alveolar bone resorption during the pathological process. Herein, we present rational design and construction a multifunctional hydrogel platform composed quaternized chitosan/oxidized dextran (QCS/OD) component with high bactericidal activity immunomodulatory carbonized polymer dots from resveratrol (RSV‐CPDs) for periodontitis treatment. Such RSV‐CPDs@QCS/OD (RCQD) hydrogels exhibit characteristics including shape adaptability, self‐healing properties, pH‐responsibility, enabling it to adapt irregularly shaped periodontal tissues, degrade gradually in mildly acidic microenvironment periodontitis, release RSV‐CPDs controlled manner. Significantly, RCQD can effectively suppress proliferation bacteria alleviate oxidative stress, relieve inflammation via activating Nrf2/NF‐κB signaling pathway, ultimately fostering favorable environment regeneration both soft hard tissue. Overall, this study highlights great potential treating by addressing practical needs tissue regeneration.

Language: Английский

Citations

1
Synthesis, bioactivity, and molecular docking studies: novel arylpiperazine derivatives as potential new-resistant AR antagonists DOI Creative Commons
Hua Jiang,

Haowei Chen,

Ya Wang

et al.

Frontiers in Chemistry, Journal Year: 2025, Volume and Issue: 13

Published: March 28, 2025

The majority of patients with androgen-dependent prostate cancer (PCa) develop resistance to hormone therapy after approximately 18-24 months androgen deprivation treatment. During this process, PCa cells progressively lose their sensitivity androgens and evolve into castration-resistant leading uncontrolled tumor growth ultimately the failure endocrine therapy. To potential anti-prostate agents, in study, we identified a novel ether-type arylpiperazine derivative as potent receptor (AR) antagonist, uncovering series effective antiproliferative compounds. derivatives (7, 11, 17, 19, 20, 21, 22, 23, 24) demonstrated strong cytotoxicity against cells, 23 showing significant antagonistic activity (Inhibition% >60) robust AR binding affinities. structure-activity relationship (SAR) these developed was discussed based on data. Docking study suggested that compound 19 mainly bind ligand pocket site through Van der Waals' force interactions. This research presents promising lead for developing anticancer agents targeting

Language: Английский

Citations

0
Niclosamide as a Potential Antivirulence Agent Disrupting Quorum Sensing in Pseudomonas aeruginosa: A Molecular and In Silico Approach DOI
Malik Suliman Mohamed, Raya Soltane, Eid Alatwi

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2025, Volume and Issue: unknown, P. 151742 - 151742

Published: April 1, 2025

Language: Английский

Citations

0
Hydrothermal synthesis and characterization of carbon quantum dots from paneer whey and evaluation of their in-vitro safety, anti-oxidant and antagonistic properties DOI
Basavaprabhu Haranahalli Nataraj, Saurabh Kumar Srivastava, S. K. Vishwakarma

et al.

Journal of Food Measurement & Characterization, Journal Year: 2025, Volume and Issue: unknown

Published: May 27, 2025

Language: Английский

Citations

0
Exploring rose absolute and phenylethyl alcohol as novel quorum sensing inhibitors in Pseudomonas aeruginosa and Chromobacterium violaceum DOI Creative Commons
Halime Çevikbaş, Seyhan Ulusoy, Neslihan Kaya Kınaytürk

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: July 8, 2024

Abstract Inter-cellular signaling, referred to as quorum sensing (QS), regulates the production of virulence factors in numerous gram-negative bacteria, such human pathogens Pseudomonas aeruginosa and Chromobacterium violaceum . QS inhibition may provide an opportunity for treatment bacterial infections. This represents initial study examine antibiofilm antivirulence capabilities rose absolute its primary component, phenylethyl alcohol. was assessed by examining extracellular exopolysaccharide synthesis, biofilm development, swarming motility P. PAO1, along with violacein C. ATCC 12472. Molecular docking analysis conducted explore mechanism which PEA inhibits QS. Our results indicate that caused decrease EPS (60.5–33.5%), (94.7–64.5%), formation (98.53–55.5%) pathogen PAO1. Violacein decreased 98.1% 62.5% (0.5 v/v %) (2 mM). Moreover, molecular revealed a promising competitive interaction between AHLs. Consequently, this offers valuable insights into potential inhibitors

Language: Английский

Citations

2
Role of fatty acids in modulating quorum sensing in Pseudomonas aeruginosa and Chromobacterium violaceum: an integrated experimental and computational analysis DOI

Allwyn Vyas Gopalakrishnan,

Abinaya Kanagaraja,

Meenakumari Sakthivelu

et al.

International Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 18, 2024

Language: Английский

Citations

2
Solvothermal Synthesis of Carbon Dots with Alkynyl Ligand for pH and Resveratrol Detection DOI
Deming Han, Jinlong Wang, Hongyi Yang

et al.

Russian Journal of General Chemistry, Journal Year: 2024, Volume and Issue: 94(7), P. 1710 - 1719

Published: July 1, 2024

Language: Английский

Citations

1
Metal and Metal Oxide Nanoparticles: Computational Analysis of Their Interactions and Antibacterial Activities Against Pseudomonas aeruginosa DOI
Badr‐Edine Sadoq, Somdutt Mujwar, Mohamed Sadoq

et al.

BioNanoScience, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 5, 2024

Language: Английский

Citations

0
Antibacterial efficacy of decorated carbon nanotubes by nano silver against pseudomonas aeruginosa DOI Creative Commons
Alaa M. Khalil,

Menna Ali Gharieb,

Shokry M Abdelaty

et al.

Materials Research Express, Journal Year: 2024, Volume and Issue: 11(7), P. 075006 - 075006

Published: July 1, 2024

Abstract Carbon nanotubes (CNTs) are considered as a promising nanomaterial for variety of applications. It has desirable physicochemical characteristics high surface area, superior mechanical and thermal strength, electrochemical activity. In this study, CNTs decorated with silver nanoparticles (AgNPs) were manufactured by an arc discharge technique. As result, etching produces stable AgNPs-CNTs aqueous suspensions pure carbon electrodes in ethanol vapor condensed water. UV- visible photometer, x-ray Diffraction, transmission electron microscope, energy dispersive characterized the synthesized AgNPs-CNTs. The crystals showed formation average 9.5 nm, intermediate length position about 75.8 nm homogeneous spherical AgNPs size 15.2 nm. antibacterial efficiency against Pseudomonas aeruginosa was established at series concentrations (45 g ml −1 : 0.0879 ) while values MIC MBC determined. levels found to be (>5.625 μ (>11.25 g/ml) respectively. bacteria cytotoxicity evaluated through LDH protein leakage levels. Treated samples 3.2 revealed significant injuries cell membrane two times greater than treated 5.0 AgNPs. Results work substantiate synergistic effect combining enhanced properties performance compared alone. using potential concentration-dependent manner. method can adapted incorporate different materials or change synthesis conditions, allowing production tailored specific

Language: Английский

Citations

0
Histological and transcriptomic insights into the interaction between grapevine and Colletotrichum viniferum DOI Creative Commons

Mengru Dou,

Yuhang Li, Yu Hao

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 16, 2024

Introduction Grape is of high economic value. Colletotrichum viniferum , a pathogen causing grape ripe rot and leaf spot, threatens production quality. Methods This study investigates the interplay between C. by Cytological transcriptome sequencing. Results Different grapevine germplasms, V. vinifera cv. Thompson Seedless (TS), labrusca accession Beaumont (B) piasezkii Liuba-8 (LB-8) were classified as highly sensitive, moderate resistant to respectively. analysis reveals distinct differences susceptible grapes post-inoculation, including faster development, longer germination tubes, normal appressoria absence white secretions in host grapevine. To understand pathogenic mechanisms sequencing was performed on “TS” identifying 236 differentially expressed genes. These included 56 effectors, 36 carbohydrate genes, 5 P450 10 genes involved secondary metabolism. Fungal effectors are known pivotal factors that modulate plant immunity affect disease development. Agrobacterium -mediated transient transformation Nicotiana benthamiana screened (CvA13877, CvA01508, CvA05621, CvA00229, CvA07043, CvA05569, CvA12648, CvA02698, CvA14071 CvA10999) inhibited INF1 (infestans 1, P. infestans PAMP elicitor) induced cell death 2 (CvA02641 CvA11478) death. Additionally, response identified related hormone signaling ( TGA PR1 ETR ERF1/2 ), resveratrol biosynthesis STS phenylpropanoid PAL COMT photosynthetic antenna proteins Lhca Lhcb transcription WRKY NAC MYB ERF GATA bHLH SBP ROS (reactive oxygen species) clearance CAT GSH POD SOD disease-related LRR RPS2 GST ). Discussion highlights potential functional diversity effectors. Our findings lay foundation for further research infection identification targets grape.

Language: Английский

Citations

0