Poly(2-(diethylamino)ethyl methacrylate)-Functionalized Carbon Nanodots as Theranostic Platforms for siRNA Delivery and Survivin Silencing in Triple-Negative Breast Cancer DOI Creative Commons
Paola Varvarà, Gennara Cavallaro, Nicolò Mauro

et al.

Biomacromolecules, Journal Year: 2025, Volume and Issue: unknown

Published: May 10, 2025

This study describes the development of carbon nanodot (CDs)-based theranostic nanocarriers that integrate gene silencing with fluorescence imaging. Nitrogen- and sulfur-doped CDs were functionalized through controlled radical surface polymerization 2-(diethylamino)ethyl methacrylate (DEAEMA), yielding self-tracking, cationic siRNA carriers CDs-pDEAEMA. The functionalization enhanced their fluorescence, broadening emission spectrum toward biologically transparent window. Fluorescent CDs-pDEAEMA effectively bound siRNA, remaining stable under physiological conditions, while in vitro studies proved hemocompatibility cytocompatibility on human dermal fibroblasts. Moreover, ability to deliver BIRC5 was demonstrated MDA-MB-231, successfully transfecting triple-negative breast cancer cells resulting an 80% reduction anti-apoptotic protein survivin. Furthermore, uptake are efficiently internalized tumor clearly detectable by imaging red region. These findings highlight potential as advanced tool for real-time tracking therapy cancer.

Language: Английский

Poly(2-(diethylamino)ethyl methacrylate)-Functionalized Carbon Nanodots as Theranostic Platforms for siRNA Delivery and Survivin Silencing in Triple-Negative Breast Cancer DOI Creative Commons
Paola Varvarà, Gennara Cavallaro, Nicolò Mauro

et al.

Biomacromolecules, Journal Year: 2025, Volume and Issue: unknown

Published: May 10, 2025

This study describes the development of carbon nanodot (CDs)-based theranostic nanocarriers that integrate gene silencing with fluorescence imaging. Nitrogen- and sulfur-doped CDs were functionalized through controlled radical surface polymerization 2-(diethylamino)ethyl methacrylate (DEAEMA), yielding self-tracking, cationic siRNA carriers CDs-pDEAEMA. The functionalization enhanced their fluorescence, broadening emission spectrum toward biologically transparent window. Fluorescent CDs-pDEAEMA effectively bound siRNA, remaining stable under physiological conditions, while in vitro studies proved hemocompatibility cytocompatibility on human dermal fibroblasts. Moreover, ability to deliver BIRC5 was demonstrated MDA-MB-231, successfully transfecting triple-negative breast cancer cells resulting an 80% reduction anti-apoptotic protein survivin. Furthermore, uptake are efficiently internalized tumor clearly detectable by imaging red region. These findings highlight potential as advanced tool for real-time tracking therapy cancer.

Language: Английский

Citations

0