Chiral carbon dot as a fluorescent stain with enantiomer-dependent cell imaging DOI Creative Commons
Zahra Hallaji,

Seyed-Omid Kalji,

Zeinab Bagheri

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 16, 2025

Chiral carbon dots (CDs) are gaining significant interest because of their potential uses in the biological fields. The effects these nanoparticles highly influenced by chiral structure. influence CD's chirality on cell imaging with red emission, particularly both fixed and live states, has not been studied yet. Here, emissive CDs (LCD DCD) were prepared through a simple one-step method tyrosine (L D) phenylenediamine (ortho). LCD DCD share very identical properties, including optical absorption, fluorescence functional groups, size, zeta potential, only difference being chirality. Red emission minimizes tissue auto-fluorescence interference enhances penetration depth, making ideal for imaging. As staining agents, LCDs effectively enter cells predominantly localize cytoplasm nucleolus but brightness is lower than able to identify nucleoli. In addition, DCDs biocompatible, exhibit much toxicity. show toxicity from 20 µg.mL-1, while this effect 150 µg.mL-1. different performance between isomers due spatial structure, which affects interaction biomolecules cellular uptake. These factors affect ability penetrate cells, within specific regions, cytotoxicity, generate signals. expected lead development smart materials enhanced efficiency various applications.

Language: Английский

Chiral carbon dot as a fluorescent stain with enantiomer-dependent cell imaging DOI Creative Commons
Zahra Hallaji,

Seyed-Omid Kalji,

Zeinab Bagheri

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 16, 2025

Chiral carbon dots (CDs) are gaining significant interest because of their potential uses in the biological fields. The effects these nanoparticles highly influenced by chiral structure. influence CD's chirality on cell imaging with red emission, particularly both fixed and live states, has not been studied yet. Here, emissive CDs (LCD DCD) were prepared through a simple one-step method tyrosine (L D) phenylenediamine (ortho). LCD DCD share very identical properties, including optical absorption, fluorescence functional groups, size, zeta potential, only difference being chirality. Red emission minimizes tissue auto-fluorescence interference enhances penetration depth, making ideal for imaging. As staining agents, LCDs effectively enter cells predominantly localize cytoplasm nucleolus but brightness is lower than able to identify nucleoli. In addition, DCDs biocompatible, exhibit much toxicity. show toxicity from 20 µg.mL-1, while this effect 150 µg.mL-1. different performance between isomers due spatial structure, which affects interaction biomolecules cellular uptake. These factors affect ability penetrate cells, within specific regions, cytotoxicity, generate signals. expected lead development smart materials enhanced efficiency various applications.

Language: Английский

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