Review of research progress on the production of cellulase from filamentous fungi

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 277, P. 134539 - 134539

Published: Aug. 8, 2024

Language: Английский

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Discovery of a novel translation-machinery-associated protein that positively correlates with cellulase production

Biotechnology for Biofuels and Bioproducts, Journal Year: 2025, Volume and Issue: 18(1)

Published: Feb. 22, 2025

Abstract Background The production of cellulases by filamentous fungi is a crucial aspect sustainable bioproduction from renewable lignocellulosic biomass. Following the transcription cellulase genes in nucleus, complex pathway involving translation, folding, and secretion required to produce extracellular cellulases. Most studies about have focused on examining transcriptional regulatory mechanisms enhancement enzyme gene levels; comparatively, little known protein translation for production. Results A translation-machinery-associated (TMA) PoTma15 was identified cellulosic Penicillium oxalicum . conserved various fungi, but not yeast, plants, or animals. All homologous proteins previously been uncharacterized. initially thought be one putative interactors factor PoXlnR, as it preyed tandem affinity purification (TAP) coupled with mass spectrometry (TAP–MS) technique using PoXlnR bait. Subsequent research revealed that associated machinery. top three are orthologs Saccharomyces cerevisiae (Tma19), elongation eIF5A, ribosomal S28, respectively. widely distributed fungal hyphae positively correlates proteins. Deleting Po tma15 (Δ ) decreased production, while overexpressing (OE increased However, Δ mutant observed downregulated transcript levels major (hemi)cellulase amylase genes, compared P wild type (WT). less affected cycloheximide, an inhibitor eukaryotic elongation, WT strain OE , suggesting stronger resistance translation-inhibiting effects cycloheximide mutant. results demonstrate affects and, consequently, Conclusions first TMA characterized used increase PoTma15’s role total suggests only can widen pathway, even engineered target improve other heterologous bioproducts cell factories future.

Language: Английский

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Endophytic and Rhizospheric Microorganisms: An Alternative for Sustainable, Organic, and Regenerative Bioinput Formulations for Modern Agriculture

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 813 - 813

Published: April 3, 2025

Large amounts of chemical fertilizers are still used to suppress pathogens and boost agricultural productivity food generation. However, their use can cause harmful environmental imbalance. Furthermore, plants typically absorb limited the nutrients provided by fertilizers. Recent studies recommending microbiota present in soil different formulations, considering that several microorganisms found nature association with a symbiotic, antagonistic, or synergistic way. This ecological alternative is positive because no undesirable significant alterations occur environment while stimulating plant nutrition development protection against damage caused control pathogens. Therefore, this review presents comprehensive discussion regarding endophytic rhizospheric interaction plants, including signaling bio-control processes concerning plant’s defense pathogenic spread. A about importance these bioinputs as microbial resource promotes sustainable methods aiming increase resilience system. In modern agriculture, manipulation through Rhizobium contributes reducing effects greenhouse gases managing nitrogen runoff decreasing nitrous oxide. Additionally, mycorrhizal fungi extend root systems, providing greater access water nutrients.

Language: Английский

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Transcriptomic and metabolic changes in Trichoderma reesei caused by mutation in xylanase regulator 1 (xyr1)

Biotechnology for Biofuels and Bioproducts, Journal Year: 2024, Volume and Issue: 17(1)

Published: July 19, 2024

Abstract Background Trichoderma reesei is known for its ability to produce large amounts of extracellular proteins and one the most important industrially used filamentous fungus. Xylanase regulator 1 (XYR1) master responsible activation cellulase hemicellulase gene expression under inducing conditions. It has been reported that strains with point mutations in certain areas xyr1 bypass need carbon source, allowing high (hemi)cellulase production even presence glucose. These also change profile produced proteins, shifting it more towards xylanase production, increase overall protein However, how these alter metabolism other cellular processes cause changes remains unclear. Results In this study, we aimed explore caused by a mutation on transcriptomic metabolic level better understand reasons behind increased both repressing glucose lactose As expected, many carbohydrate-active enzyme (CAZy) genes was mutant their induction higher The strain built biomass during growth compared wild type strain. Genes involved oxidoreductive D-galactose catabolism pathway were upregulated strain, potentially contributing efficient utilization lactose. addition CAZy genes, clustering enrichment analysis showed over-representation mitochondria-related Gene Ontology terms clusters where mutant, indicating mitochondria play role altered state associated mutation. Metabolomics revealed free tyrosine abundant all measured timepoints, whereas multiple fatty acids less Conclusions results contribute in-depth knowledge T. physiology growing sources gives new insights function XYR1. vast data generated serve as source targets improved production.

Language: Английский

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Engineering cellulolytic fungi for efficient lignocellulosic biomass hydrolysis: advances in mutagenesis, gene editing, and nanotechnology with CRISPR-Cas innovations

Deleted Journal, Journal Year: 2024, Volume and Issue: 6(12)

Published: Dec. 4, 2024

The increasing global energy demand and environmental concerns have highlighted the need for sustainable renewable sources. Lignocellulosic biomass (LCB), rich in cellulose, hemicellulose, lignin, is a promising resource biofuel production. However, recalcitrant nature of lignin poses significant challenge by obstructing effective LCB decomposition. This review provides comprehensive analysis recent advancements genetic metabolic engineering techniques, focusing on directed random mutagenesis to enhance cellulase production fungi. It explores how these techniques can overcome challenges degradation improve conversion efficiency. Lignin's high resistance degradation, due its complex association with cellulose hemicelluloses, necessitates development advanced fungal strains through mutagenesis. Fungi, which are efficient degraders, benefit from practices enzyme address pollution burning LCB. emphasizes cellulolytic fungi mutagenesis, gene-editing, synthetic biology, highlighting CRISPR-Cas innovations nanoparticle-based delivery systems precise application. also discusses role transcription factors boosting practical applications in-situ biodegradation. Effective implementation could foster economy mitigate negative impacts current agricultural practices.

Language: Английский

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