Dehydrodiisoeugenol alleviates palmitate-induced mitochondrial dysfunction in human vascular smooth muscle cells through the activation of SIRT1-mediated Drp1 deacetylation DOI Creative Commons
Jianjun Zhao, Zhiyun Shu, Xiangjun Li

et al.

Lipids in Health and Disease, Journal Year: 2025, Volume and Issue: 24(1)

Published: May 24, 2025

Dehydrodiisoeugenol (Deh) has demonstrated positive effects in the prevention and treatment of cardiovascular disease (CVD) caused by lipid overload, but its specific mechanism action remains poorly understood. The aim this study was to investigate possible mechanisms which Deh modulates mitochondrial dysfunction induced palmitate (PA) vascular smooth muscle cells (VSMCs). A PA-induced high-fat model VSMCs established, effect PA on function detected evaluating oxidative stress apoptosis cells, as well function. expression dynamin-related protein 1 (Drp1) immunofluorescence immunoprecipitation. key targets for mitochondria-related diseases were screened bioinformatics analysis molecular docking techniques. Finally, role Silent information regulator (SIRT1) explored administrating SIRT1 activator (CAY10602, CAY) inhibitor (JGB1741, JGB). results showed that concentration-dependently increased VSMCs, while modulating acetylation Drp1, promoting ectopia, thereby inducing dysfunction. Bioinformatics indicated may be a target diseases. Follow-up experiments revealed significantly inhibited suppressing Drp1 reducing ectasia, an achieved regulating SIRT1. able inhibit ectopia through activation SIRT1, inhibiting ameliorating pathological processes, such cellular apoptosis, maintaining stable functions.

Language: Английский

Anoikis Resistance in Cancer: Mechanisms, Therapeutic Strategies, Potential Targets, and Models for Enhanced Understanding DOI
Pallab Shaw, Arpan Dey Bhowmik, Mohan Shankar Gopinatha Pillai

et al.

Cancer Letters, Journal Year: 2025, Volume and Issue: unknown, P. 217750 - 217750

Published: April 1, 2025

Language: Английский

Citations

0
Dehydrodiisoeugenol alleviates palmitate-induced mitochondrial dysfunction in human vascular smooth muscle cells through the activation of SIRT1-mediated Drp1 deacetylation DOI Creative Commons
Jianjun Zhao, Zhiyun Shu, Xiangjun Li

et al.

Lipids in Health and Disease, Journal Year: 2025, Volume and Issue: 24(1)

Published: May 24, 2025

Dehydrodiisoeugenol (Deh) has demonstrated positive effects in the prevention and treatment of cardiovascular disease (CVD) caused by lipid overload, but its specific mechanism action remains poorly understood. The aim this study was to investigate possible mechanisms which Deh modulates mitochondrial dysfunction induced palmitate (PA) vascular smooth muscle cells (VSMCs). A PA-induced high-fat model VSMCs established, effect PA on function detected evaluating oxidative stress apoptosis cells, as well function. expression dynamin-related protein 1 (Drp1) immunofluorescence immunoprecipitation. key targets for mitochondria-related diseases were screened bioinformatics analysis molecular docking techniques. Finally, role Silent information regulator (SIRT1) explored administrating SIRT1 activator (CAY10602, CAY) inhibitor (JGB1741, JGB). results showed that concentration-dependently increased VSMCs, while modulating acetylation Drp1, promoting ectopia, thereby inducing dysfunction. Bioinformatics indicated may be a target diseases. Follow-up experiments revealed significantly inhibited suppressing Drp1 reducing ectasia, an achieved regulating SIRT1. able inhibit ectopia through activation SIRT1, inhibiting ameliorating pathological processes, such cellular apoptosis, maintaining stable functions.

Language: Английский

Citations

0