Label-Free Liquid Crystal Aptamer Sensors Based on Single-Stranded Nucleic Acid π-Structures for Detecting cTnI DOI

Yunpeng Xue,

Ruyu Shi,

Liang‐Yu Chen

et al.

Langmuir, Journal Year: 2024, Volume and Issue: 40(51), P. 26988 - 26996

Published: Dec. 11, 2024

Cardiac troponin I (cTnI) is a highly sensitive and important serological marker for clinical diagnosis of myocardial injury. Its rapid detection crucial the early cardiovascular diseases such as acute infarction. In this study, based on nucleic acid molecular hybridization aptamer-specific binding to target molecules, label-free liquid crystal aptamer sensor single-stranded π-structures was developed applied quantitative cTnI. The CP1 CP2 oligonucleotide chains, complementary bases at both ends aptamer, are covalently bonded substrate via APTES GA-mediated molecules. forms π-structure with through hybridization, serving molecule capture probe. When cTnI present in system, chains competitively bind causing breakdown within sensor. This reinstates long-range ordered alignment 5CB molecules sensor, enabling measurement variations optical images. Experimental results show that range 0.01 25 ng/mL concentration, there linear correlation between brightness area coverage (Br) polarized light microscopy images logarithm coefficient (

Language: Английский

Intelligent point of care testing for medicine diagnosis DOI Creative Commons

Qiya Gao,

Shuang Li

Deleted Journal, Journal Year: 2024, Volume and Issue: 2(1)

Published: Jan. 1, 2024

Abstract Intelligent point‐of‐care testing (iPOCT) is a rapidly advancing technology that combines molecular detection and bio‐sensing techniques to achieve instant accurate analysis through advanced sensors intelligent algorithms. Molecular technologies, such as nucleic acid isothermal amplification, biochip microfluidics, enable rapid of the chemical composition concentration samples, thereby determining their presence. Bio‐sensing utilizes recognition properties biomolecules in conjunction with detect specific or biological processes. iPOCT holds broad prospects for application field healthcare expected enhance sensitivity, accuracy, intelligence further development. In this review, we delineate developmental journey iPOCT, followed by discussion on principles technology, well design devices health monitoring disease diagnosis. Furthermore, showcased illustrative examples applications diagnosis, highlighting integration wearable smartphones attaining more precise personalized diagnostic results.

Language: Английский

Citations

18
A novel integrated lateral flow immunoassay platform for the detection of cardiac troponin I using hierarchical dendritic copper-nickel nanostructures DOI

Yuxin Tan,

Shirong Zhang,

Yilei Liu

et al.

Talanta, Journal Year: 2024, Volume and Issue: 277, P. 126332 - 126332

Published: May 28, 2024

Language: Английский

Citations

7
Cu2+-triggered wavelength-tunable real-time in situ fluorogenic immunoassay for detection of cTnI in human serum DOI
Hong Yang,

Shanshan Zhu,

Lihua Pang

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 406, P. 135388 - 135388

Published: Jan. 28, 2024

Language: Английский

Citations

5
Rapid diagnosis of acute myocardial infarction through integrated microfluidic chips for detection of characteristic targets DOI Creative Commons

Yaling Yuan,

Yue Zhong,

Yanbin Yang

et al.

Analytical Biochemistry, Journal Year: 2024, Volume and Issue: 689, P. 115502 - 115502

Published: March 6, 2024

Myoglobin (Myo), creatine kinase-MB (CKMB), and cardiac troponin I (cTnI) are crucial biomarkers for diagnosing acute myocardial infarction (AMI) The accurate rapid detection of these three targets can greatly improve the prognosis AMI patients. Herein, this study developed a microfluidic immunofluorescence method that detect all in 10-15 min. Ultrasonic atomization spray technology used to modify surface injection-molded chip (MFC), which effectively solves problem biological cross-linking antibody immobilization on MFC surface. In addition, it improves hydrophilicity surface, thus enhancing fluid self-driving effect. linear response towards Myo, CKMB cTnI range from 5 ng/mL 500 ng/mL, 1 70 0.05 30 respectively. intra-batch precision is ≤ 10%, inter-batch 15%. Furthermore, shows good consistency compared with BECKMAN ACCESS2 chemiluminescent immunoanalyzer. present work provides an diagnostic high sensitivity, repeatability, accuracy simple operation, satisfy needs clinical diagnosis, promising application prospects.

Language: Английский

Citations

4
Advances in microfluidics for biomedical engineering applications DOI
Mahsa Sadat Razavi, Ali Nematollahzadeh

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 301 - 327

Published: Jan. 1, 2025

Language: Английский

Citations

0
A Capillary-Based Microfluidic Chip Prepared via Multiple Coating and Used for a Multiplex Immunoassay DOI
Jiajia Liu, Huan Xu, Yulin Deng

et al.

Talanta, Journal Year: 2025, Volume and Issue: 288, P. 127713 - 127713

Published: Feb. 10, 2025

Language: Английский

Citations

0
Label-Free Liquid Crystal Biosensor Based on Split Aptamers for Detecting Serum Myoglobin DOI

Shaowei Ju,

Shu Zhang, Tingting Yuan

et al.

Langmuir, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

Myoglobin (Mb) is a critical cytoplasmic heme protein and one of the earliest detectable biomarkers following acute myocardial infarction (MI). The rapid precise detection serum Mb holds significant clinical value. This study capitalizes on advantages split aptamer (Apt) sequence, which short effectively minimizes false-positive signals caused by secondary structure probe. It constructs "sandwich"-structured liquid crystal biosensor with an "Apt-target molecule-Apt" configuration for label-free Mb. Apt fragments were immobilized surface glass substrate as capture probes (CP) using triethoxybutanal silane (TEA) linker molecules. When present, other fragment, signal probe (SP), specifically binds reassociates CP, restoring original stem-loop Apt, thereby inducing change in alignment 5CB leads to variations brightness color optical polarizing microscope image, facilitating quantitative method capable detecting concentrations ranging from 0.05 130 ng/mL, minimum limit 0.045 ng/mL. sandwich biosensing offers several advantages, including detection, operational simplicity, high specificity. demonstrates potential biomarkers.

Language: Английский

Citations

0
Quantitative measurement of acute myocardial infarction cardiac biomarkers by “All-in-One” immune microfluidic chip for early diagnosis of myocardial infarction DOI

Zhaokui Zeng,

Huimin Li, Qi Li

et al.

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2024, Volume and Issue: 315, P. 124256 - 124256

Published: April 10, 2024

Language: Английский

Citations

3
Investigation on the linear cooling method of microfluidic chip based on thermoelectric cooler DOI
Dongfang Sun, Han Xue, Haoqing Wang

et al.

Energy, Journal Year: 2024, Volume and Issue: 308, P. 132933 - 132933

Published: Aug. 23, 2024

Language: Английский

Citations

3
Innovative, Flexible, and Miniaturized Microfluidic Paper-Based Plasmonic Chip for Efficient Near-Infrared Metal Enhanced Fluorescence Biosensing and Imaging DOI
Andreea Campu,

Ilinca Muresan,

Ana-Maria Craciun

et al.

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(48), P. 55925 - 55937

Published: Nov. 20, 2023

The implementation of metal enhanced fluorescence (MEF) as an efficient detection tool, especially in the near-infrared region electromagnetic spectrum, is a rather new direction for diagnostic analytical technologies. In this context, we propose novel microfluidic plasmonic design based on paper MEF "proof-of-concept" biotin–streptavidin recognition interaction. Our made use benefits gold nanobipyramids (AuBPs), considering strong field present at their sharp tips, and filter to operate natural channel due excellent wicking abilities. calligraphed paper, obtained using commercial pen filled with AuBPs, was integrated robust sandwich optically transparent polydimethylsiloxane chip, exhibiting portability flexibility while preserving chip's properties. To place Alexa 680 fluorophore optimal distance from nanobipyramid substrate, human IgG-anti-IgG-conjugated biotin reaction employed. Thus, upon capture 680-conjugated streptavidin by biotinylated system, 1.3-fold average enhancement fluorophore's emission determined bulk measurements. However, local factor considerably higher values spanning 5 6.3, proven mapping under both re-scan microscopy lifetime imaging, endorsing proposed feasibility biosensing well high-resolution bioimaging. Finally, versatility our chip demonstrated adapting protocol cardiac troponin I biomarker detection, validated 10 plasma samples collected pediatric patients corroborated conventional ELISA assay.

Language: Английский

Citations

7