Progress in Medical Devices,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 31, 2024
In
the
field
of
bioanalysis,
integration
magnetic
beads
and
biosensors
provides
a
protein
detection
platform
with
high
separation
efficiency
sensitivity.
The
superparamagnetism
beads,
combined
surface
functional
modifications,
forms
basis
for
selectively
capturing
effectively
separating
target
proteins.
Additionally,
sensitivity
specificity
ensure
precise
quantitative
analysis
captured
This
article
systematically
reviews
synthesis
strategies
functionalized
methods
proteins
nucleic
acids,
as
well
current
technical
challenges
future
development
directions.
Cancer Cell International,
Journal Year:
2024,
Volume and Issue:
24(1)
Published: May 30, 2024
Abstract
The
advancement
of
nanoscience
and
material
design
in
recent
times
has
facilitated
the
creation
point-of-care
devices
for
cancer
diagnosis
biomolecule
sensing.
Exosomes
(EXOs)
facilitate
transfer
bioactive
molecules
between
cells
diverse
local
distant
microenvironments,
thereby
contributing
to
progression
metastasis.
Specifically,
EXOs
derived
from
are
likely
function
as
biomarkers
early
detection
due
genetic
or
signaling
alterations
they
transport
payload
within
origin.
It
been
verified
that
circulate
steadily
bodily
secretions
contain
a
variety
information
indicates
tumor.
However,
acquiring
molecular
interactions
regarding
presented
significant
technical
challenges
their
nanoscale
nature
high
heterogeneity.
Colorimetry,
surface
plasmon
resonance
(SPR),
fluorescence,
Raman
scattering
examples
optical
techniques
utilized
quantify
exosomal
biomarkers,
including
lipids,
proteins,
RNA,
DNA.
Many
optically
active
nanoparticles
(NPs),
predominantly
carbon-based,
inorganic,
organic,
composite-based
nanomaterials,
have
employed
biosensing
technology.
exceptional
physical
properties
exhibited
by
carbon
NPs,
noble
metal
magnetic
progress
development
nanobiosensors
intended
originating
tumors.
Following
summary
biogenesis,
biological
functions,
biomarker
value
known
EXOs,
this
article
provides
an
update
on
methodologies
currently
under
investigation.
In
conclusion,
we
propose
some
potential
enhancements
biosensors
detecting
EXO,
utilizing
various
NP
materials
such
silicon
graphene
oxide
(GO),
quantum
dots
(QDs).
Microchimica Acta,
Journal Year:
2025,
Volume and Issue:
192(4)
Published: March 5, 2025
The
review
article
provides
a
short
introduction
to
exosomes
with
the
focus
use
as
disease
markers
itself
(i.e.
their
concentration
or
presence
of
some
specific
receptors)
source
biomarkers
such
proteins
and
metabolites.
In
detail,
we
are
discussing
various
methods
exosome
isolation
main
paper
is
on
affinity
capture
exosomes,
since
them
can
be
applied
sub-populations
produced
by
organs.
comprehensive
overview
magnetic
(bio)affinity
detection
exosomal
cargo
using
different
ligands
antibodies,
DNA
aptamers,
peptides,
glycan-based
recognition,
transferrin-based
approaches,
based
recognition
phospholipids
other
approaches
including
electrostatic
interactions.
in
detail
key
analytical
clinical
parameters
form
an
extensive
table
summarising
outcomes
published
last
two
years
(2023-2024).
Finally,
also
conclusions
sections
pros
cons
for
and/or
determination
content.
Bioengineering,
Journal Year:
2024,
Volume and Issue:
11(12), P. 1292 - 1292
Published: Dec. 19, 2024
Fluorescence
is
a
remarkable
property
exhibited
by
many
chemical
compounds
and
biomolecules.
has
revolutionized
analytical
biomedical
sciences
due
to
its
wide-ranging
applications
in
diagnostic
tools
of
biological
environmental
importance.
Fluorescent
molecules
are
frequently
employed
drug
delivery,
optical
sensing,
cellular
imaging,
biomarker
discovery.
Cancer
global
challenge
fluorescence
agents
can
function
as
well
monitoring
tools,
both
during
early
tumor
progression
treatment
monitoring.
Many
fluorescent
be
found
their
natural
form,
but
recent
developments
synthetic
chemistry
molecular
biology
have
allowed
us
synthesize
tune
that
would
not
otherwise
exist
nature.
Naturally
derived
generally
more
biocompatible
environmentally
friendly.
They
also
modified
cost-effective
target-specific
ways
with
the
help
tools.
Understanding
unique
structures
photophysical
properties
key
harnessing
full
potential
research.
As
discovery
efforts
require
rigorous
characterization
pharmacokinetics
pharmacodynamics,
fluorescence-based
detection
accelerates
understanding
interactions
via
vitro
vivo
assays.
Herein,
we
provide
review
products
analogs
exhibit
used
probes,
detailing
properties.
We
provided
some
insights
into
relationships
between
Finally,
discussed
science,
mainly
study
cancer
cells
research,
highlighting
pivotal
role
advancing
biomarkers,
cell
biosensing
technologies,
targeting
ligands
diagnosis
tumors.
Chinese Journal of Chromatography,
Journal Year:
2025,
Volume and Issue:
43(5), P. 424 - 433
Published: May 1, 2025
Exosomes
play
crucial
intercellular-communication
roles
and
regulate
various
cellular
physiological
processes.
They
are
considered
potential
biomarkers
for
the
early
diagnosis
of
cancers
other
diseases.
Therefore,
detecting
isolating
exosomes
with
specific
functions
has
significant
clinical
implications.
Moreover,
development
low-cost,
highly
sensitive
recognition
elements
identifying
is
essential
advancing
disease
treatment.
Nucleic
acid
aptamers
single-stranded
DNA
or
RNA
molecules
capable
specifically
binding
to
targets
produced
through
systematic
evolution
ligands
by
exponential
enrichment
(SELEX)
technique.
Such
stable,
chemically
synthesizable,
exhibit
high
affinities
specificities,
applicable
a
broad
range
targets,
which
endow
them
unique
advantages.
Currently,
that
target
have
been
used
in
variety
research
fields,
including
cell
imaging,
drug
delivery,
However,
selecting
precisely
identify
significantly
challenging
owing
complex
structures
exosome
their
heterogeneity.
Consequently,
obtaining
high-performance
requires
efficient
screening
techniques.
This
review
first
summarizes
selection
strategies
key
exosome-aptamer
screening.
Furthermore,
it
outlines
main
methods
techniques
currently
screen
aptamers,
includes
five
techniques:
magnetic
bead-SELEX,
microfluidic-SELEX,
nitrocellulose-SELEX,
cell-SELEX,
capillary
electrophoresis-SELEX.
The
separation
principles,
advantages,
limitations,
latest
applications
these
discussed
detail.
finally
addresses
current
challenges
associated
provides
insight
into
future
directions.
