Portable microfluidic devices for monitoring antibiotic resistance genes in wastewater DOI

Rida Feng,

Kang Mao, Hua Zhang

et al.

Microchimica Acta, Journal Year: 2024, Volume and Issue: 192(1)

Published: Dec. 21, 2024

Language: Английский

Phosphorothioated oligonucleotides on gold-coated electrospun polymeric fibers for electrochemical genosensors DOI Creative Commons

Anca Aldea,

Melania Onea, Elena Matei

et al.

Electrochimica Acta, Journal Year: 2025, Volume and Issue: unknown, P. 146006 - 146006

Published: March 1, 2025

Language: Английский

Citations

0
Electrochemical genosensors on-a-chip: Applications in early diagnosis of pathogens DOI Creative Commons
Amin Dehghan, Mohammad Javad Kiani, Ali Gholizadeh

et al.

Sensors and Actuators Reports, Journal Year: 2025, Volume and Issue: unknown, P. 100335 - 100335

Published: May 1, 2025

Language: Английский

Citations

0
Development and application of a highly sensitive quadruple droplet digital PCR method for simultaneous quantification of sulfonamide resistance genes DOI Creative Commons

Xirong Yin,

Jiayuan Nie,

Huifang Tian

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: May 21, 2025

Sulfonamide resistance genes ( sul genes) have a high detection rate and strong transmissibility. Therefore, there is an urgent need to develop more efficient methods enhance the monitoring of genes. Current analytical are insufficient for simultaneous accurate quantification all sulfonamides To overcome this limitation, quadruple method was established by integrating droplet digital PCR (ddPCR) with ratio-based probe-mixing strategy, achieving sensitive sul1 , sul2 sul3 sul4 in diverse matrices. Correspondingly, primers probes were meticulously designed rigorously validated, critical parameters ddPCR such as annealing temperature, concentrations systematically optimized. As results, demonstrates excellent sensitivity limits (LOD) ranging from 3.98 6.16 copies/reaction, good repeatability (coefficient variation <25%), adequately meeting requirement quantification. Furthermore, new applied across 115 samples, including human feces, animal-derived foods, sewage surface water, positive rates 100% 99.13% 93.91% 68.70% concentration non-detection 2.14 × 10 9 copies/g. In summary, developed has potential serve tool

Language: Английский

Citations

0
Aptasensors for the Detection of Environmental Contaminants of High Concern in Water Bodies: A Systematic Review DOI Creative Commons
Eduardo C. Reynoso, Patrick Severin Sfragano,

Mario González-Perea

et al.

Chemosensors, Journal Year: 2024, Volume and Issue: 12(4), P. 59 - 59

Published: April 9, 2024

With the advancement of technology and increasing industrial activity, anthropogenic contaminants are currently detected where there is no record their presence or insufficient information about toxicological impact. Consequently, not sufficiently robust local global regulations, ecotoxicological human health risks critical, they may be routinely monitored despite being ubiquitous. The interest in studying environmental contaminants, including micropollutants emerging complex water samples has grown last decade. Due to concentrations which typically found environment rapid dispersion, detection procedures for these substances must capable measuring very low concentrations. Many efforts have been made improve remediation develop novel analytical methods determination. Although several reliable standard techniques monitoring, pollutant contamination requires simple inexpensive massive, situ monitoring campaigns. In this regard, biosensors emerged as devices with high selectivity, sensitivity, easy operation, short analysis times. Aptasensors based on a nucleic acid recognition element (aptamer). synthetic nature, stability, production, aptamers frequently employed bioassays. This work presents systematic review trends using aptasensors detecting present samples, well estimation potential technological contribution might give monitoring.

Language: Английский

Citations

3
Skin-contact Wearable Electrochemical Biosensing Tools: a perspective for the Well-being of Neurological Patients DOI Creative Commons

Ilaria Antonia Vitale,

Neri Fuochi,

Daniele Martella

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 180, P. 117965 - 117965

Published: Sept. 7, 2024

Language: Английский

Citations

0
Sustainable hybrid nanocomposites of Au nanoparticles modified carboxylated nanographene oxide: Electrochemical characterisation and estrone detection DOI
Patrick Severin Sfragano, Serena Laschi, Fabio Vischio

et al.

Microchemical Journal, Journal Year: 2024, Volume and Issue: 207, P. 112145 - 112145

Published: Nov. 13, 2024

Language: Английский

Citations

0
Portable microfluidic devices for monitoring antibiotic resistance genes in wastewater DOI

Rida Feng,

Kang Mao, Hua Zhang

et al.

Microchimica Acta, Journal Year: 2024, Volume and Issue: 192(1)

Published: Dec. 21, 2024

Language: Английский

Citations

0