Enhancing Early Breast Cancer Detection with Ape1-Triggered Oligonucleotide Probes and Graphene Oxide: The Impact of Variable AP Site Modification on Sensitivity and Specificity DOI

Xue zhao Yu,

Sujian Cao,

Xuexian Deng

et al.

Published: Jan. 1, 2024

Language: Английский

Electrochemical Biosensors for the Detection of Exosomal microRNA Biomarkers for Early Diagnosis of Neurodegenerative Diseases DOI Creative Commons

Jiacheng Yu,

Runzhi Zhou,

Shan Liu

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 9, 2025

Early and precise diagnosis of neurodegenerative disorders like Alzheimer's (AD) Parkinson's (PD) is crucial for slowing their progression enhancing patient outcomes. Exosomal microRNAs (miRNAs) are emerging as promising biomarkers due to ability reflect the diseases' pathology, yet low abundance poses significant detection hurdles. This review article delves into burgeoning field electrochemical biosensors, designed exosomal miRNA biomarkers. Electrochemical biosensors offer a compelling solution, combining sensitivity required detect low-abundance with specificity needed discern profiles distinctive neural pathological states. We explore operational principles these including transduction mechanisms that facilitate detection. The also summarizes advancements in nanotechnology, signal enhancement, bioreceptor anchoring, microfluidic integration improve sensor accuracy. evidence use disease analyzed, focusing on clinical impact, diagnostic precision, obstacles faced practical applications. Their potential point-of-care testing regulatory considerations market entry discussed. Looking toward future, highlights forthcoming innovations might revolutionize early processes. impressive sensitivity, specificity, compatibility, track become instrumental diseases, possibly transforming care prognosis.

Language: Английский

Citations

2
Dumbbell Probe Initiated Multi-Rolling Circle Amplification Assisted CRISPR/Cas12a for Highly Sensitive Detection of Clinical microRNA DOI
Xudan Shen, Ziwei Lin,

Xianfeng Jiang

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 264, P. 116676 - 116676

Published: Aug. 14, 2024

Language: Английский

Citations

4
Enhancing early breast cancer detection with APE1-triggered oligonucleotide probes and graphene oxide: The impact of variable AP site modification on sensitivity and specificity DOI
Xiaoxiao Yu,

Sujian Cao,

Xuexian Deng

et al.

Talanta, Journal Year: 2025, Volume and Issue: 287, P. 127505 - 127505

Published: Jan. 24, 2025

Language: Английский

Citations

0
Engineering Multi-activator-encoded DNA Nanonet to Accelerate CRISPR-Cas12a Activation for Rapid and Sensitive Electrochemiluminescence Bioassay DOI
Mei‐Ling Liu, Yi Li, Wenjing Yang

et al.

Talanta, Journal Year: 2025, Volume and Issue: 288, P. 127724 - 127724

Published: Feb. 11, 2025

Language: Английский

Citations

0
One-Pot Detection of miRNA by Dual Rolling Circle Amplification at Ambient Temperature with High Specificity and Sensitivity DOI Creative Commons
Wenhua Sun,

Kunling Hu,

Ziting Song

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(5), P. 317 - 317

Published: May 15, 2025

Rolling circle amplification (RCA) at ambient temperature is prone to false positive signals during nucleic acid detection, which makes it challenging establish an efficient RCA detection method. The are primarily caused by binding of non-target acids the circular single-stranded template, leading non-specific amplification. Here, we present method for miRNA 37 °C using two ssDNAs, each formed ligating intramolecularly nick (without any splint) in a secondary structure. specific target recognition realized utilizing low concentrations (0.1 nM) ssDNA1 (C1). A phosphorothioate modification G*AATTC on C1 generate primer extension self-generated rolling (PG-RCA). fragmented products used as primers following that serves signal ssDNA2 (C2). Notably, absence splints and concentration significantly inhibits binding, thus minimizing signals. high (10 C2 carry out linear (LRCA), highly specific. This strategy demonstrates good response 0.01–100 pM with limit 7.76 fM (miR-155). Moreover, can distinguish single-nucleotide mismatch miRNA, enabling rapid one-pot °C. Accordingly, this performs specificity sensitivity. approach suitable clinical serum sample analysis offers developing biosensors diagnostic tools.

Language: Английский

Citations

0
APE1: A critical focus in neurodegenerative conditions DOI Open Access

Qianxiong He,

Yì Wáng, Zhao Feng

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 179, P. 117332 - 117332

Published: Aug. 26, 2024

The global growth of the aging population has resulted in an increased prevalence neurodegenerative diseases, characterized by progressive loss central nervous system (CNS) structure and function. Given high incidence debilitating nature there is urgent need to identify potential biomarkers novel therapeutic targets thereof. Apurinic/apyrimidinic endonuclease 1 (APE1), been implicated several as having a significant role. Abnormal APE1 expression observed conditions including Alzheimer's disease, stroke, amyotrophic lateral sclerosis, Parkinson's Huntington's epilepsy. However, whether this dysregulation protective or harmful remains unclear. This review aims comprehensively current understanding involvement diseases.

Language: Английский

Citations

1
Enhancing Early Breast Cancer Detection with Ape1-Triggered Oligonucleotide Probes and Graphene Oxide: The Impact of Variable AP Site Modification on Sensitivity and Specificity DOI

Xue zhao Yu,

Sujian Cao,

Xuexian Deng

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0