TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 118120 - 118120
Published: Dec. 1, 2024
Language: Английский
Foods, Journal Year: 2025, Volume and Issue: 14(7), P. 1115 - 1115
Published: March 24, 2025
Food safety is directly related to human health and has attracted intense attention all over the world. Surface-enhanced Raman scattering (SERS), as a rapid selective technique, been widely applied in monitoring food safety. SERS substrates, an essential factor for sensing design, greatly influence analytical performance. Currently, nanostructure-based substrates have garnered significant interest due their excellent merits improving sensitivity, specificity, stability, holding great potential accurate of contaminants complex matrices. This review summarizes fundamentals spectroscopy used nanostructures designing platform, including precious metal nanoparticles, metal-organic frameworks, polymers, semiconductors. Moreover, it introduces mechanisms applications enhancing signals hazardous substances, such foodborne bacteria, pesticide veterinary drug residues, additives, illegal adulterants, packaging material contamination. Finally, with continuous progress nanostructure technology improvement technology, its application prospect testing will be broader.
Language: Английский
Citations
1
TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: 185, P. 118173 - 118173
Published: Feb. 3, 2025
Language: Английский
Citations
0
Journal of Raman Spectroscopy, Journal Year: 2025, Volume and Issue: unknown
Published: April 24, 2025
ABSTRACT Researchers have long been interested in nucleic acid detection technology. Surface‐enhanced Raman spectroscopy (SERS) is distinguished by its high sensitivity, minimal sample volume, resistance to fluorescence interference, cost‐effectiveness, and rapidity compared conventional analysis methods. The clustered regularly interspaced short palindromic repeats/CRISPR‐associated protein (CRISPR/Cas), a novel gene editing tool, has garnered significant interest due precise identification isothermal advantages. Integrating the CRISPR/Cas system's specific capabilities with high‐sensitivity fingerprinting properties of SERS offers sensitive, ultra‐low rapid, straightforward method for detecting new modalities. This review delineates components characteristics system, encompassing three Cas proteins (Cas9, Cas12, Cas13) technologies derived from CRISPR/Cas, namely, enzymatic reporter unlocking (SHERLOCK) DNA endonuclease‐targeted CRISPR trans reporters (DETECTR). Advancements CRISPR/Cas‐SERS‐based assays were emphasized, traditional non‐nucleic tests. Examples encompass microfluidics/microdroplet‐based CRISPR/Cas‐SERS non‐amplified based on CRISPR/Cas‐SERS, so on.
Language: Английский
Citations
0
SLAS TECHNOLOGY, Journal Year: 2024, Volume and Issue: unknown, P. 100229 - 100229
Published: Dec. 1, 2024
Nitroreductase (NTR) plays a critical role in the oxygen-deficient environment of anoxic tumor cells, and its identification is crucial for diagnosis treatment cancer. This research introduces an innovative Surface Enhanced Raman Scattering (SERS) probe, created by attaching p-nitrothiophenol (p-NTP) to gold nanoparticles (Au NPs). probe leverages specific enzymatic reaction NTR hypoxic status, utilizing decreased NADH. The activity transforms nitroaromatic compounds into aromatic amines, which then reflected as measurable shift SERS signal probe. novel approach allows accurate quantification NTR, with sensitivity reaching detection threshold less than 0.02 μg/mL. probe's non-toxic nature superior biocompatibility facilitate use direct investigations A549 cells under reduced oxygen levels. We also applied this method xenograft model. results demonstrate marked increase levels tissues conditions, highlighting significance nanoprobe enhancing cancer diagnostics, helping medical doctors making decisions more swiftly effectively.
Language: Английский
Citations
1
TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 118120 - 118120
Published: Dec. 1, 2024
Language: Английский
Citations
0