Spermine accumulation via spermine synthase promotes tumor cell proliferation in head and neck squamous cell carcinoma

BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 5, 2025

Head and neck squamous cell carcinoma (HNSCC) is among the most aggressive malignancies, underscoring need for early diagnosis to improve patient outcomes. Tumor-derived exosomes, which can be non-invasively obtained reflect metabolic state of tumors in real-time, are under increasing investigation their diagnostic potential. Herein we analyzed metabolite differences serum, tissues from patients with HNSCC identify potential biomarkers clinical relevance. Non-targeted metabolomics based on liquid chromatography-mass spectrometry was employed quantify metabolites exosome, tissue samples 11 six without cancer. The profiles were through univariate multivariate statistical methods, differential analysis, pathway enrichment analysis. We identified three 45 33 tissues. Notably, exhibited significant disruptions protein amino acid metabolism. Spermine exclusively detected exosomes HNSCC. hypothesize that spermine extracellularly secreted by malignant cells via subsequently enters bloodstream. Moreover, synthase highly expressed Knocking down markedly impaired proliferation migration. This study provides a preliminarily characterization profile highlights its synthetic pathways as therapeutic targets. Future studies warranted elucidate mechanism action explore utility development.

Language: Английский

“Sample-In, Result-Out” liquid biopsy chip based on immunomagnetic separation and CRISPR detection for multiplex analysis of exosomal microRNAs

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: unknown, P. 117460 - 117460

Published: April 1, 2025

Language: Английский