Expression of nanobodies in Arabidopsis thaliana strengthens the absorption capacity of triclosan from growth media DOI

Weixia Liu,

Mengya Li, Xiangyu Zhang

et al.

Physiologia Plantarum, Journal Year: 2025, Volume and Issue: 177(2)

Published: March 1, 2025

Triclosan (TCS) is a broad-spectrum antimicrobial disinfectant widely used in pharmaceuticals and personal care products (PPCPs). Due to the extensive usage of PPCPs, TCS inevitably entered environment pose harmful effects on ecosystem. Phytoremediation an attractive approach remove from environment. In this study, gene encoding anti-TCS nanobody was transformed into Arabidopsis thaliana (A. thaliana) enhance absorption capacity TCS. Nanobodies are small antibody fragments (ca. 15 kDa) derived variable domain camelid heavy-chain-only antibodies. We constructed two transgenic lines, T-S-C line with expression throughout plant T-S-P dominant roots, were constructed. The nanobodies A. alleviated phytotoxicity exhibited significantly stronger tolerance toxicity than wild type (WT), either solid medium system or hydroponics system. Under stress TCS, seedlings both plants increase root length fresh weight compared those WT. Moreover, presence activities superoxide dismutase, peroxidase, catalase, glutathione higher concentration absorbed increased by 50.0% 24.1%, 55.6% 38.0%, respectively, This study provides proof principle that transforming represents novel technology improve efficiency phytoremediation for environmental pollutants.

Language: Английский

Expression of nanobodies in Arabidopsis thaliana strengthens the absorption capacity of triclosan from growth media DOI

Weixia Liu,

Mengya Li, Xiangyu Zhang

et al.

Physiologia Plantarum, Journal Year: 2025, Volume and Issue: 177(2)

Published: March 1, 2025

Triclosan (TCS) is a broad-spectrum antimicrobial disinfectant widely used in pharmaceuticals and personal care products (PPCPs). Due to the extensive usage of PPCPs, TCS inevitably entered environment pose harmful effects on ecosystem. Phytoremediation an attractive approach remove from environment. In this study, gene encoding anti-TCS nanobody was transformed into Arabidopsis thaliana (A. thaliana) enhance absorption capacity TCS. Nanobodies are small antibody fragments (ca. 15 kDa) derived variable domain camelid heavy-chain-only antibodies. We constructed two transgenic lines, T-S-C line with expression throughout plant T-S-P dominant roots, were constructed. The nanobodies A. alleviated phytotoxicity exhibited significantly stronger tolerance toxicity than wild type (WT), either solid medium system or hydroponics system. Under stress TCS, seedlings both plants increase root length fresh weight compared those WT. Moreover, presence activities superoxide dismutase, peroxidase, catalase, glutathione higher concentration absorbed increased by 50.0% 24.1%, 55.6% 38.0%, respectively, This study provides proof principle that transforming represents novel technology improve efficiency phytoremediation for environmental pollutants.

Language: Английский

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