A comprehensive review: synergizing stem cell and embryonic development knowledge in mouse and human integrated stem cell-based embryo models DOI Creative Commons
Cathérine Dupont

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: April 22, 2024

Mammalian stem cell-based embryo models have emerged as innovative tools for investigating early embryogenesis in both mice and primates. They not only reduce the need sacrificing but also overcome ethical limitations associated with human research. Furthermore, they provide a platform to address scientific questions that are otherwise challenging explore vivo . The usefulness of model depends on its fidelity replicating development, efficiency reproducibility; all essential addressing biological queries quantitative manner, enabling statistical analysis. Achieving such requires robust systems demand extensive optimization efforts. A profound understanding pre- post-implantation cellular plasticity, lineage specification, existing is imperative making informed decisions constructing these models. This review aims highlight differences development cell biology between humans, assess how variances influence formation partially fully integrated models, identify critical challenges field.

Language: Английский

Adult bi-paternal offspring generated through direct modification of imprinted genes in mammals DOI Creative Commons
Zhikun Li, Libin Wang, Leyun Wang

et al.

Cell stem cell, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Imprinting abnormalities pose a significant challenge in applications involving embryonic stem cells, induced pluripotent and animal cloning, with no universal correction method owing to their complexity stochastic nature. In this study, we targeted these defects at source-embryos from same-sex parents-aiming establish stable, maintainable imprinting pattern de novo mammalian cells. Using bi-paternal mouse embryos, which exhibit severe are typically non-viable, introduced frameshift mutations, gene deletions, regulatory edits 20 key imprinted loci, ultimately achieving the development of fully adult animals, albeit relatively low survival rate. The findings provide strong evidence that primary barrier unisexual reproduction mammals. Moreover, approach can significantly improve developmental outcomes for cells cloned opening promising avenues advancements regenerative medicine.

Language: Английский

Citations

3
Fetal fibroblast heterogeneity defines dermal architecture during human embryonic skin development DOI

Noriko Morioka,

Clarisse Gânier, Fiona M. Watt

et al.

Journal of Investigative Dermatology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

2
Neurulation of the cynomolgus monkey embryo achieved from 3D blastocyst culture DOI Creative Commons
Jinglei Zhai,

Yanhong Xu,

Haifeng Wan

et al.

Cell, Journal Year: 2023, Volume and Issue: 186(10), P. 2078 - 2091.e18

Published: May 1, 2023

Neural tube (NT) defects arise from abnormal neurulation and result in the most common birth worldwide. Yet, mechanisms of primate remain largely unknown due to prohibitions on human embryo research limitations available model systems. Here, we establish a three-dimensional (3D) prolonged vitro culture (pIVC) system supporting cynomolgus monkey development 7 25 days post-fertilization. Through single-cell multi-omics analyses, demonstrate that pIVC embryos form three germ layers, including primordial cells, proper DNA methylation chromatin accessibility through advanced gastrulation stages. In addition, immunofluorescence confirms neural crest formation, NT closure, progenitor regionalization. Finally, transcriptional profiles morphogenetics resemble key features similarly staged vivo embryos. This work therefore describes study non-human embryogenesis early neurulation.

Language: Английский

Citations

33
Incorporation of Decidual Stromal Cells Derived Exosomes in Sodium Alginate Hydrogel as an Innovative Therapeutic Strategy for Advancing Endometrial Regeneration and Reinstating Fertility DOI
Yuxiang Liang, Qizhi Shuai, Xiao Zhang

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(13)

Published: Feb. 7, 2024

Abstract Intrauterine adhesion (IUA) stands as a prevalent medical condition characterized by endometrial fibrosis and scar tissue formation within the uterine cavity, resulting in infertility and, severe cases, recurrent miscarriages. Cell therapy, especially with stem cells, offers an alternative to surgery, but concerns about uncontrolled differentiation tumorigenicity limit its use. Exosomes, more stable immunogenicity‐reduced than parent have emerged promising avenue for IUA treatment. In this study, novel approach has been proposed wherein exosomes originating from decidual stromal cells (DSCs) are encapsulated sodium alginate hydrogel (SAH) scaffolds repair damage restore fertility mouse model. Current results demonstrate that situ injection of DSC‐derived (DSC‐exos)/SAH into cavity capability induce angiogenesis, initiate mesenchymal‐to‐epithelial transformation (MET), facilitate collagen fiber remodeling dissolution, promote regeneration, enhance receptivity, contribute recovery fertility. RNA sequencing advanced bioinformatics analysis reveal miRNA enrichment exosomes, potentially supporting repair. This finding elucidates how DSC‐exos/SAH mechanistically fosters ablation, endometrium recovery, holding potential introduce treatment offering invaluable insights realm regenerative medicine.

Language: Английский

Citations

13
Modeling early gastrulation in human blastoids with DNA methylation patterns of natural blastocysts DOI
Han Xie, Chenrui An, Bing Bai

et al.

Cell stem cell, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

1
Role of spindle assembly checkpoint proteins in gametogenesis and embryogenesis DOI Creative Commons

Renju Pun,

Brian J. North

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 12

Published: Jan. 22, 2025

The spindle assembly checkpoint (SAC) is a surveillance mechanism that prevents uneven segregation of sister chromatids between daughter cells during anaphase. This essential regulatory aneuploidy which can lead to various congenital defects observed in newborns. Many studies have been carried out elucidate the role proteins involved SAC as well function gametogenesis and embryogenesis. In this review, we discuss regulating both meiotic mitotic cell division along with several factors influence strength species. Finally, outline consequences their absence or insufficiency on proper embryogenesis vivo .

Language: Английский

Citations

1
Germline stem cells in human DOI Creative Commons
Hanhua Cheng,

Dantong Shang,

Rongjia Zhou

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2022, Volume and Issue: 7(1)

Published: Oct. 2, 2022

Abstract The germline cells are essential for the propagation of human beings, thus survival mankind. stem cells, as a unique cell type, generate various states germ and then differentiate into specialized spermatozoa ova, producing offspring, while self-renew to more cells. Abnormal development often causes severe diseases in humans, including infertility cancer. Primordial (PGCs) first emerge during early embryonic development, migrate gentile ridge, join formation gonads. In males, they spermatogonial which give rise via meiosis from onset puberty, females, female (FGSCs) retain stemness ovary initiate oocytes. cell-like (PGCLCs) can be induced vitro or pluripotent this review, we focus on current advances these adult PGCLCs provide an overview molecular mechanisms underlying differentiation outline their physiological functions, pathological implications, clinical applications.

Language: Английский

Citations

31
The placenta: epigenetic insights into trophoblast developmental models of a generation-bridging organ with long-lasting impact on lifelong health DOI
Myriam Hemberger, Wendy Dean

Physiological Reviews, Journal Year: 2023, Volume and Issue: 103(4), P. 2523 - 2560

Published: May 12, 2023

The placenta is a unique organ system that functionally combines both maternal and fetal cell types with distinct lineage origins. Normal placentation critical for developmental progression reproductive success. Although the best known its nutrient supply function to fetus, genetic experiments in mice highlight also pivotal directing proper formation of specific organs. These roles underscore importance pregnancy outcome lifelong health span, which makes it essential better understand molecular processes governing placental development find adequate models study it. In this review, we provide an overview instructional role epigenome dictating fate decisions specifically trophoblast lineage. We then focus on recent advances exploring stem organoid reflecting feto-maternal interface humans much-improved tools events early development. discuss cells derived from as well those artificially induced resemble placenta, how they can be combined embryonic endometrial uterus reconstitute implantation site. allude exciting prospects these harnessed biomedicine enhance our understanding pathological underpinnings complications patient-specific manner, ultimately facilitate therapeutic approaches tissue- organ-based regenerative medicine.

Language: Английский

Citations

22
Micro-RNAs in Human Placenta: Tiny Molecules, Immense Power DOI Creative Commons
Meiyuan Jin,

Qiang Xu,

Jiayong Li

et al.

Molecules, Journal Year: 2022, Volume and Issue: 27(18), P. 5943 - 5943

Published: Sept. 13, 2022

Micro-RNAs (miRNAs) are short non-coding single-stranded RNAs that modulate the expression of various target genes after transcription. The and distribution kinds miRNAs have been characterized in human placenta during different gestational stages. identified recognized as key mediators regulation placental development maintenance pregnancy. Aberrant is associated with compromised pregnancies humans, dysregulation those contributes to occurrence related diseases pregnancy, such pre-eclampsia (PE), fetal growth restriction (FGR), diabetes mellitus (GDM), recurrent miscarriage, preterm birth (PTB) small-for-gestational-age (SGA). Thus, having a better understanding functions pregnancy thereby developing novel drugs targeting could be potentially promising method prevention treatment relevant future. Here, we summarize current knowledge pattern function diseases.

Language: Английский

Citations

23
Development of a Microfluidic Chip Powered by EWOD for In Vitro Manipulation of Bovine Embryos DOI Creative Commons
Adriana Karcz, Ann Van Soom, Katrien Smits

et al.

Biosensors, Journal Year: 2023, Volume and Issue: 13(4), P. 419 - 419

Published: March 25, 2023

Digital microfluidics (DMF) holds great potential for the alleviation of laboratory procedures in assisted reproductive technologies (ARTs). The electrowetting on dielectric (EWOD) technology provides dynamic culture conditions vitro that may better mimic natural embryo microenvironment. Thus far, EWOD microdevices have been proposed gamete and handling mice analyzing human secretome. This article presents development first microfluidic chip utilizing designed manipulation bovine embryos vitro. prototype sustains cell cycles manipulated individually chips during (IVC). Challenges related to fabrication as well its application IVC accordance with adapted on-chip protocol are thoroughly discussed, future directions DMF ARTs indicated.

Language: Английский

Citations

16