bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 20, 2024
Abstract
The
progression
of
the
cell
cycle
is
regulated
by
expression
specific
genes
and
fluctuations
in
cellular
metabolic
states.
Previous
research
has
employed
cycle-based
transcriptomics,
proteomics,
metabolomics
analyses
to
identify
cycle-dependent
changes
at
gene
expression,
protein,
levels.
However,
role
protein
compartmentalization
regulating
function,
coupled
with
evidence
that
enzymes
can
localize
nucleus
influence
chromatin
states,
suggests
nuclear
metabolism
may
play
a
progression.
In
this
study,
we
developed
an
approach
resolve
during
unbiased
systematic
manner.
This
was
achieved
integrating
fluorescent
reporters
mass
spectrometry
imaging.
Our
investigation
focused
on
revealed
phosphatidylinositol
localizes
Moreover,
disruption
affects
distribution
4,5-bisphosphate,
alters
number
morphology
nucleoli,
influences
maintenance
distinct
heterochromatin
states
throughout
cycle.
Finally,
given
established
link
between
methionine
synthesis,
as
well
differential
impact
observed
histone
marks
when
perturbed,
proposed
pools
be
involved
decorate
Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: June 26, 2024
Birt-Hogg-Dubé
(BHD)
syndrome
patients
are
uniquely
susceptible
to
all
renal
tumour
subtypes.
The
underlying
mechanism
of
carcinogenesis
is
unclear.
To
study
cancer
development
in
BHD,
we
used
human
proximal
kidney
(HK2)
cells
and
found
that
long-term
folliculin
Frontiers in Cell and Developmental Biology,
Journal Year:
2024,
Volume and Issue:
12
Published: Sept. 27, 2024
The
transcription
factor
p53
(encoded
by
TP53
)
plays
diverse
roles
in
human
development
and
disease.
While
best
known
for
its
role
tumor
suppression,
signaling
also
influences
mammalian
triggering
cell
fate
decisions
response
to
a
wide
variety
of
stresses.
After
over
4
decades
study,
new
pathway
that
triggers
activation
mitotic
delays
was
recently
identified.
Termed
the
surveillance
or
stopwatch
pathway,
USP28
53BP1
proteins
activate
delayed
progression
control
promote
genomic
stability.
In
this
Minireview,
I
discuss
identification,
potential
neurodevelopmental
disorders
cancer,
as
well
explore
outstanding
questions
about
function,
regulation
use
biomarker
anti-mitotic
therapies.
Journal of Biomedical Materials Research Part A,
Journal Year:
2024,
Volume and Issue:
113(1)
Published: Dec. 13, 2024
ABSTRACT
Cardiovascular
diseases
(CVDs)
are
the
leading
causes
of
death
worldwide,
with
approx.
Twenty
million
deaths
in
2021.
implants
among
most
used
biomaterials
clinical
world.
However,
poor
endothelialisation
and
rapid
thrombosis
remains
a
challenge.
Simple
chemical
surface
modification
techniques
can
be
to
steer
biological
interactions
without
affecting
bioimplants'
overall
bulk
characteristics
such
as
radiopacity
flexibility.
Although
silanes
well
studied
for
protein
cell
interactions,
methodical
investigation
cardiac
endothelial
(EC)
alongside
smooth
muscle
(SMC)
mimic
natural
arterial
environments
has
been
limited.
In
this
study,
these
cells
have
investigated
on
surfaces
functionalized
methyl,
amine,
thiol,
methacrylate,
fluorine
organosilane
groups.
Cardiac
EC
SMC
growth
was
metabolic
activity,
time
lapse
imaging,
immunofluorescent
staining
techniques.
The
results
demonstrated
that
tested
able
selectively
regulate
viability
cells.
Aminosilane
modified
displayed
2‐fold
higher
activity
HUVEC
less
HCASMC
lines,
compared
tissue
culture
plastic
controls.
amino‐modification
outperformed
all
other
chemistries
terms
ability
promote
proliferation
ECs,
while
importantly
reducing
SMCs.
This
report
demonstrates
aminosilane
potential
utilized
novel
cardiovascular
implants,
which
could
improve
integration
short
possibly
longer‐term.
findings
study
suggest
specific
modifications
enhance
minimizing
cells,
often
associated
thrombosis.
highlights
carefully
engineered
outcomes
implants.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 20, 2024
Abstract
The
progression
of
the
cell
cycle
is
regulated
by
expression
specific
genes
and
fluctuations
in
cellular
metabolic
states.
Previous
research
has
employed
cycle-based
transcriptomics,
proteomics,
metabolomics
analyses
to
identify
cycle-dependent
changes
at
gene
expression,
protein,
levels.
However,
role
protein
compartmentalization
regulating
function,
coupled
with
evidence
that
enzymes
can
localize
nucleus
influence
chromatin
states,
suggests
nuclear
metabolism
may
play
a
progression.
In
this
study,
we
developed
an
approach
resolve
during
unbiased
systematic
manner.
This
was
achieved
integrating
fluorescent
reporters
mass
spectrometry
imaging.
Our
investigation
focused
on
revealed
phosphatidylinositol
localizes
Moreover,
disruption
affects
distribution
4,5-bisphosphate,
alters
number
morphology
nucleoli,
influences
maintenance
distinct
heterochromatin
states
throughout
cycle.
Finally,
given
established
link
between
methionine
synthesis,
as
well
differential
impact
observed
histone
marks
when
perturbed,
proposed
pools
be
involved
decorate
