Quantifying Intracellular Mechanosensitive Response upon Spatially Defined Mechano-Chemical Triggering DOI Creative Commons
Elaheh Zare‐Eelanjegh, Renard Lewis, Ines Lüchtefeld

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: May 6, 2025

Abstract The mechanotransduction process relies on the interaction of mechanical and biochemical cues, transmitting cellular forces to intracellular organelles activate pathways elicit responses. This involves mechanoresponsive components like actin filaments, microtubules (MTs), lamin meshwork. Fluidic force microscopy (FluidFM), a force-controlled micropipette allows for manipulation intact cells mechanically chemically, providing novel approach study mechanotransmission in situ . FluidFM combined with fluorescence lifetime imaging (FLIM), enables high-resolution mapping tension dynamics. Here, we used varying nuclear lamina compositions explore lamina’s role initiating mechanoresponse external cues. We found that A-type B-type lamins trigger distinctly, contributing elasticity, whereas influence viscous response. Moreover, MTs underwent adaptation assisted releasing A/C knockout (KO) cells, contrasting healthy where aid preserve locally rather than transferring it. research provides insights into dynamic supports targeted therapies stress-related diseases.

Language: Английский

TMEM63B functions as a mammalian hyperosmolar sensor for thirst DOI
Wenjie Zou,

Siqi Deng,

Xingyu Chen

et al.

Neuron, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Optical Tweezer-Driven Mechanotransduction: Probing pN-Scale Forces and Calcium-Mediated Redox Signaling in Single Endothelial Cells DOI
Yuyao Li, Haodong Li, Yawen Zheng

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Endothelial cells (ECs) regulate vascular function by converting mechanical forces into biochemical signals; however, the molecular mechanisms of pN-scale mechanotransduction remain elusive. Here, we develop an optical tweezer-integrated confocal microscopy system that allows precise, noninvasive manipulation cell membrane localization with stimuli within 0-100 pN range while monitoring Ca2+-mediated NO/ROS redox signaling in situ single ECs under varying force parameters. We show stimulation regulates extracellular Ca2+ influx, triggering downstream production NO and ROS, which subsequently affects intracellular homeostasis. Key mechanosensitive ion channels (e.g., Piezo1 TRPV4) cytoskeletal components F-actin) facilitate force-induced signaling. further delineate roles tension-dominant versus hybrid tension-tether models mechanotransduction, revealing their differential engagement transmission pathways. This mechanistic framework establishes direct connections between input characteristics redox-regulated

Language: Английский

Citations

0
Quantifying Intracellular Mechanosensitive Response upon Spatially Defined Mechano-Chemical Triggering DOI Creative Commons
Elaheh Zare‐Eelanjegh, Renard Lewis, Ines Lüchtefeld

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: May 6, 2025

Abstract The mechanotransduction process relies on the interaction of mechanical and biochemical cues, transmitting cellular forces to intracellular organelles activate pathways elicit responses. This involves mechanoresponsive components like actin filaments, microtubules (MTs), lamin meshwork. Fluidic force microscopy (FluidFM), a force-controlled micropipette allows for manipulation intact cells mechanically chemically, providing novel approach study mechanotransmission in situ . FluidFM combined with fluorescence lifetime imaging (FLIM), enables high-resolution mapping tension dynamics. Here, we used varying nuclear lamina compositions explore lamina’s role initiating mechanoresponse external cues. We found that A-type B-type lamins trigger distinctly, contributing elasticity, whereas influence viscous response. Moreover, MTs underwent adaptation assisted releasing A/C knockout (KO) cells, contrasting healthy where aid preserve locally rather than transferring it. research provides insights into dynamic supports targeted therapies stress-related diseases.

Language: Английский

Citations

0