Animal Model Screening for Hyperlipidemic ICR Mice DOI Open Access
Xingtong Chen,

Yunyue Zhou,

Jinbiao Yang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2142 - 2142

Published: Feb. 27, 2025

This study aimed to establish a hyperlipidemia model in ICR mice using homemade high-fat diet. It further investigated hyperlipidemia-related indicators control and at various feeding durations determine the optimal time frame for successful establishment. Sixteen male were introduced intervals of 3 weeks, starting from weeks 0, 3, 6, 9, 12. The group was fed standard diet, while received diet induce hyperlipidemia. Blood lipid related indices detected 15 weeks. liver, scapular fat, abdominal epididymal fat harvested calculate organ index. contents T-CHO, TG, TBA liver measured. HE staining used observe pathological changes tissue white adipose tissue, Oil Red O droplets tissue. mRNA protein expression SREBP-2, insig1, HMGCR, LXRα, ABCA1, CYP7A1 by RT-qPCR Western Blot. In group, blood levels significantly increased 9th week, aligning with indicative Insig-1, markedly elevated 9 remained relatively stable thereafter. provides reliable reference determining establishment models vivo animal experiments.

Language: Английский

Association between FIB-4 index and lower extremity arterial disease in MASLD patients: a cross-sectional study DOI Creative Commons
Chunxia Zhang, Yuchen Ying,

Yuanhui Ru

et al.

Lipids in Health and Disease, Journal Year: 2025, Volume and Issue: 24(1)

Published: March 20, 2025

Metabolic dysfunction-associated steatotic liver disease (MASLD) is associated with an elevated risk of cardiovascular conditions, such as lower extremity arterial (LEAD). The Fibrosis-4 (FIB-4) index, a non-invasive marker fibrosis, may have predictive value for LEAD in patients MASLD. This study aimed to explore the association between FIB-4 and cohort cross-sectional included 481 participants MASLD, selected from comprehensive health check-up database. Participants were categorized into three groups based on their index (< 1.3, 1.3–2.66, > 2.66) underwent duplex ultrasonography diagnose LEAD. Logistic regression models employed evaluate LEAD, adjusting demographic, metabolic, lipid-related factors. Subgroup analyses performed by sex, age, diabetes mellitus status, hypertension, dyslipidemia, smoking status. prevalence increased levels, 51.3% low group 86.5% high (p < 0.001). In fully adjusted models, higher levels significantly (adjusted odds ratio [OR]: 3.54, 95% confidence interval [CI]: 1.39–9.01) compared group. As continuous variable, each unit increase was 66% likelihood OR: 1.66, CI: 1.12–2.26, P did not reveal significant interactions (P interaction 0.05). Higher are independently MASLD patients, although subgroup interactions. suggests that further studies larger sample sizes needed these relationships more comprehensively.

Language: Английский

Citations

1
miR-125b-5p regulates FFA-induced hepatic steatosis in L02 cells by targeting estrogen-related receptor alpha DOI Creative Commons

Gao Fen,

Yanhua Ma, Chun I. Yu

et al.

Gene, Journal Year: 2025, Volume and Issue: unknown, P. 149419 - 149419

Published: March 1, 2025

Language: Английский

Citations

0
Cardiovascular-liver-metabolic health: Time to integrate liver assessment into cardiology practice? DOI

Luan C V Lima,

Lubna Al‐Sharif, Matheus Souza

et al.

Trends in Cardiovascular Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Animal Model Screening for Hyperlipidemic ICR Mice DOI Open Access
Xingtong Chen,

Yunyue Zhou,

Jinbiao Yang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2142 - 2142

Published: Feb. 27, 2025

This study aimed to establish a hyperlipidemia model in ICR mice using homemade high-fat diet. It further investigated hyperlipidemia-related indicators control and at various feeding durations determine the optimal time frame for successful establishment. Sixteen male were introduced intervals of 3 weeks, starting from weeks 0, 3, 6, 9, 12. The group was fed standard diet, while received diet induce hyperlipidemia. Blood lipid related indices detected 15 weeks. liver, scapular fat, abdominal epididymal fat harvested calculate organ index. contents T-CHO, TG, TBA liver measured. HE staining used observe pathological changes tissue white adipose tissue, Oil Red O droplets tissue. mRNA protein expression SREBP-2, insig1, HMGCR, LXRα, ABCA1, CYP7A1 by RT-qPCR Western Blot. In group, blood levels significantly increased 9th week, aligning with indicative Insig-1, markedly elevated 9 remained relatively stable thereafter. provides reliable reference determining establishment models vivo animal experiments.

Language: Английский

Citations

0